RNA interference for CFTR attenuates lung fluid absorption at birth in rats
<p>Abstract</p> <p>Background</p> <p>Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs.</p> <p>M...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-07-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/9/1/55 |
| _version_ | 1818001084711436288 |
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| author | Folkesson Hans G Koshy Shyny Li Tianbo |
| author_facet | Folkesson Hans G Koshy Shyny Li Tianbo |
| author_sort | Folkesson Hans G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs.</p> <p>Methods</p> <p>Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C<sub>2</sub>), we measured CFTR and ENaC expression, extravascular lung water, and mortality.</p> <p>Results</p> <p>αENaC and CFTR mRNA and protein decreased by ~80% and ~85%, respectively, following αENaC and CFTR silencing. Extravascular lung water and mortality increased after αENaC and CFTR-silencing. In pSi-C<sub>2</sub>-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells αENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced αENaC mRNA and protein. αENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein.</p> <p>Conclusion</p> <p>Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.</p> |
| first_indexed | 2024-04-14T03:30:10Z |
| format | Article |
| id | doaj.art-767be51c52744bc4abab4bb948a8a1d6 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-14T03:30:10Z |
| publishDate | 2008-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-767be51c52744bc4abab4bb948a8a1d62022-12-22T02:15:00ZengBMCRespiratory Research1465-99212008-07-01915510.1186/1465-9921-9-55RNA interference for CFTR attenuates lung fluid absorption at birth in ratsFolkesson Hans GKoshy ShynyLi Tianbo<p>Abstract</p> <p>Background</p> <p>Small interfering RNA (siRNA) against αENaC (α-subunit of the epithelial Na channel) and CFTR (cystic fibrosis transmembrane conductance regulator) was used to explore ENaC and CTFR function in newborn rat lungs.</p> <p>Methods</p> <p>Twenty-four hours after trans-thoracic intrapulmonary (ttip) injection of siRNA-generating plasmid DNA (pSi-0, pSi-4, or pSi-C<sub>2</sub>), we measured CFTR and ENaC expression, extravascular lung water, and mortality.</p> <p>Results</p> <p>αENaC and CFTR mRNA and protein decreased by ~80% and ~85%, respectively, following αENaC and CFTR silencing. Extravascular lung water and mortality increased after αENaC and CFTR-silencing. In pSi-C<sub>2</sub>-transfected isolated DLE cells there were attenuated CFTR mRNA and protein. In pSi-4-transfected DLE cells αENaC mRNA and protein were both reduced. Interestingly, CFTR-silencing also reduced αENaC mRNA and protein. αENaC silencing, on the other hand, only slightly reduced CFTR mRNA and protein.</p> <p>Conclusion</p> <p>Thus, ENaC and CFTR are both involved in the fluid secretion to absorption conversion around at birth.</p>http://respiratory-research.com/content/9/1/55 |
| spellingShingle | Folkesson Hans G Koshy Shyny Li Tianbo RNA interference for CFTR attenuates lung fluid absorption at birth in rats Respiratory Research |
| title | RNA interference for CFTR attenuates lung fluid absorption at birth in rats |
| title_full | RNA interference for CFTR attenuates lung fluid absorption at birth in rats |
| title_fullStr | RNA interference for CFTR attenuates lung fluid absorption at birth in rats |
| title_full_unstemmed | RNA interference for CFTR attenuates lung fluid absorption at birth in rats |
| title_short | RNA interference for CFTR attenuates lung fluid absorption at birth in rats |
| title_sort | rna interference for cftr attenuates lung fluid absorption at birth in rats |
| url | http://respiratory-research.com/content/9/1/55 |
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