Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison
Abstract Several polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long‐term failure. There is a need to improve such scaffolds' design and physical...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-01-01
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| Series: | Macromolecular Materials and Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mame.202200426 |
| _version_ | 1797743176500379648 |
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| author | Tânia Peixoto Daniel Silva Miguel Rodrigues Miguel Neto Rui Silva Maria C. Paiva Liliana Grenho Maria Helena Fernandes Maria A. Lopes |
| author_facet | Tânia Peixoto Daniel Silva Miguel Rodrigues Miguel Neto Rui Silva Maria C. Paiva Liliana Grenho Maria Helena Fernandes Maria A. Lopes |
| author_sort | Tânia Peixoto |
| collection | DOAJ |
| description | Abstract Several polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long‐term failure. There is a need to improve such scaffolds' design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids' surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X‐ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples' surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale‐up, showing potential for application in the final hybrid medical device. |
| first_indexed | 2024-03-12T14:51:45Z |
| format | Article |
| id | doaj.art-767cffe84a624edeb073976cc6f0a888 |
| institution | Directory Open Access Journal |
| issn | 1438-7492 1439-2054 |
| language | English |
| last_indexed | 2024-03-12T14:51:45Z |
| publishDate | 2023-01-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj.art-767cffe84a624edeb073976cc6f0a8882023-08-15T09:10:20ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542023-01-013081n/an/a10.1002/mame.202200426Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental ComparisonTânia Peixoto0Daniel Silva1Miguel Rodrigues2Miguel Neto3Rui Silva4Maria C. Paiva5Liliana Grenho6Maria Helena Fernandes7Maria A. Lopes8REQUIMTE‐LAQV, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias Porto 4200‐465 PortugalREQUIMTE‐LAQV, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias Porto 4200‐465 PortugalREQUIMTE‐LAQV, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias Porto 4200‐465 PortugalCICECO, Departamento de Engenharia de Materiais e Cerâmica Universidade de Aveiro Aveiro 3810‐193 PortugalCICECO, Departamento de Engenharia de Materiais e Cerâmica Universidade de Aveiro Aveiro 3810‐193 PortugalInstituto de Polímeros e Compósitos, Departamento de Engenharia de Polímeros Universidade do Minho Guimarães 4800‐058 PortugalLaboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária Universidade do Porto Rua Dr. Manuel Pereira da Silva Porto 4200‐393 PortugalLaboratory for Bone Metabolism and Regeneration, Faculdade de Medicina Dentária Universidade do Porto Rua Dr. Manuel Pereira da Silva Porto 4200‐393 PortugalREQUIMTE‐LAQV, Departamento de Engenharia Metalúrgica e Materiais, Faculdade de Engenharia Universidade do Porto Rua Dr. Roberto Frias Porto 4200‐465 PortugalAbstract Several polymers are researched for tendon repair as polyethylene terephthalate (PET) and polylactic acid (PLA). These are biocompatible and useful in scaffolding repair though with minimal success due to long‐term failure. There is a need to improve such scaffolds' design and physical–chemical nature. This work concerns surface functionalization of polymeric braids (PET and PLA) that fulfill the high mechanical demands of tissues such as tendons. The functionalization aims to incorporate amine groups in the braids' surface, improve cell adhesion, and consequently, the poor healing rate of these tissues and the biointegration of the braids. Two approaches are compared: the direct application of NH3 plasma and the surface grafting of EDA after O2 plasma activation. X‐ray photoelectron spectroscopy (XPS) shows that amine groups are effectively introduced onto the samples' surfaces. Besides, the plasma parameters chosen do not compromise the topography and tensile behavior of the braids. Resazurin assay and scanning electron microscopy show that the NH3 treatment improves cell–biomaterial interaction as improved cell adhesion and proliferation are observed. Both approaches are safe for biomedical applications. The NH3 plasma approach is more environmentally friendly, faster, and easier to scale‐up, showing potential for application in the final hybrid medical device.https://doi.org/10.1002/mame.202200426ethylenediaminehybrid medical device (PET/PLA)NH3 plasmasurface functionalizationtissue repair |
| spellingShingle | Tânia Peixoto Daniel Silva Miguel Rodrigues Miguel Neto Rui Silva Maria C. Paiva Liliana Grenho Maria Helena Fernandes Maria A. Lopes Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison Macromolecular Materials and Engineering ethylenediamine hybrid medical device (PET/PLA) NH3 plasma surface functionalization tissue repair |
| title | Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison |
| title_full | Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison |
| title_fullStr | Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison |
| title_full_unstemmed | Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison |
| title_short | Amination of Polymeric Braid Structures to Improve Tendon Healing: An Experimental Comparison |
| title_sort | amination of polymeric braid structures to improve tendon healing an experimental comparison |
| topic | ethylenediamine hybrid medical device (PET/PLA) NH3 plasma surface functionalization tissue repair |
| url | https://doi.org/10.1002/mame.202200426 |
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