Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang
Sanjoin-tang (SJIT) is an ancient oriental medicine prescription listed in the <i>Jinguiyaolue</i> that is mainly used for the treatment of primary insomnia. This study was conducted to develop and validate an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS...
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| Format: | Article |
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MDPI AG
2023-07-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/7/411 |
| _version_ | 1797587453368860672 |
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| author | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_facet | Chang-Seob Seo Hyeun-Kyoo Shin |
| author_sort | Chang-Seob Seo |
| collection | DOAJ |
| description | Sanjoin-tang (SJIT) is an ancient oriental medicine prescription listed in the <i>Jinguiyaolue</i> that is mainly used for the treatment of primary insomnia. This study was conducted to develop and validate an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous analysis method for the quality control of SJIT using 18 target compounds. The 18 analytes were separated on an Acquity UPLC BEH C<sub>18</sub> column maintained at 45 °C using a mobile phase composed of distilled water and acetonitrile. The MS system was used to simultaneously detect all analytes using the multiple reaction monitoring (MRM) method of Xevo TQ-XS coupled with an electrospray ionization source. The concentrations of the 18 analytes investigated in the SJIT samples ranged from below the limit of detection to 9.553 mg/g. In conclusion, the validated UPLC–MS/MS MRM analysis method can be used to obtain basic data to establish chemical-nonclinical linkage efficacy and for the clinical research and quality evaluation of SJIT. |
| first_indexed | 2024-03-11T00:39:09Z |
| format | Article |
| id | doaj.art-767e6f9d122d4be1b65a88b0e99695ff |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T00:39:09Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-767e6f9d122d4be1b65a88b0e99695ff2023-11-18T21:21:21ZengMDPI AGSeparations2297-87392023-07-0110741110.3390/separations10070411Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-TangChang-Seob Seo0Hyeun-Kyoo Shin1KM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaKM Science Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Republic of KoreaSanjoin-tang (SJIT) is an ancient oriental medicine prescription listed in the <i>Jinguiyaolue</i> that is mainly used for the treatment of primary insomnia. This study was conducted to develop and validate an ultra-performance liquid chromatography with tandem mass spectrometry (UPLC–MS/MS) simultaneous analysis method for the quality control of SJIT using 18 target compounds. The 18 analytes were separated on an Acquity UPLC BEH C<sub>18</sub> column maintained at 45 °C using a mobile phase composed of distilled water and acetonitrile. The MS system was used to simultaneously detect all analytes using the multiple reaction monitoring (MRM) method of Xevo TQ-XS coupled with an electrospray ionization source. The concentrations of the 18 analytes investigated in the SJIT samples ranged from below the limit of detection to 9.553 mg/g. In conclusion, the validated UPLC–MS/MS MRM analysis method can be used to obtain basic data to establish chemical-nonclinical linkage efficacy and for the clinical research and quality evaluation of SJIT.https://www.mdpi.com/2297-8739/10/7/411simultaneous quantitative analysistraditional herbal prescriptionSanjoin-tangUPLC–MS/MS |
| spellingShingle | Chang-Seob Seo Hyeun-Kyoo Shin Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang Separations simultaneous quantitative analysis traditional herbal prescription Sanjoin-tang UPLC–MS/MS |
| title | Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang |
| title_full | Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang |
| title_fullStr | Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang |
| title_full_unstemmed | Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang |
| title_short | Validation of the Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Simultaneous Analysis of Eighteen Compounds in the Traditional Herbal Prescription, Sanjoin-Tang |
| title_sort | validation of the ultra performance liquid chromatography with tandem mass spectrometry method for simultaneous analysis of eighteen compounds in the traditional herbal prescription sanjoin tang |
| topic | simultaneous quantitative analysis traditional herbal prescription Sanjoin-tang UPLC–MS/MS |
| url | https://www.mdpi.com/2297-8739/10/7/411 |
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