Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria
Segregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of XcvΔscpBXv (ScpBXv knockout mutan...
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| Format: | Article |
| Language: | English |
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Hanrimwon Publishing Company
2017-12-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720608 |
| _version_ | 1818598075170553856 |
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| author | Hye-Jee Park Sang-Wook Han |
| author_facet | Hye-Jee Park Sang-Wook Han |
| author_sort | Hye-Jee Park |
| collection | DOAJ |
| description | Segregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of XcvΔscpBXv (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried out and the abundance of 187 proteins was altered. Among them, diverse transcriptional regulators involved in biofilm formation and virulence were abundant in the wild-type. We further showed that biofilm formation of XcvΔscpBXv was reduced. This study provides new insights into the functions of ScpBXv in bacterial replication and biofilm formation, which may contribute to the virulence of Xcv. |
| first_indexed | 2024-12-16T11:57:56Z |
| format | Article |
| id | doaj.art-767f3fa497d64cb49ce3024591b1f06d |
| institution | Directory Open Access Journal |
| issn | 1598-2254 |
| language | English |
| last_indexed | 2024-12-16T11:57:56Z |
| publishDate | 2017-12-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-767f3fa497d64cb49ce3024591b1f06d2022-12-21T22:32:31ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542017-12-0133660260710.5423/PPJ.NT.07.2017.0151PPJ.NT.07.2017.0151Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoriaHye-Jee Park0Sang-Wook Han1Department of Integrative Plant Science, Chung-Ang University, Anseong 17546, KoreaDepartment of Integrative Plant Science, Chung-Ang University, Anseong 17546, KoreaSegregation and condensation protein B (ScpB) is essential for replication and segregation in living organisms. Here, we reported the functions of ScpBXv (ScpB-like protein in Xanthomonas campestris pv. vesicatoria) using phenotypic and proteomic analyses. Growth of XcvΔscpBXv (ScpBXv knockout mutant) was reduced under both slow and fast growth conditions in rich medium, but comparable to this of the wild-type in plant-mimic conditions. Interestingly, the mutant was significantly less virulent than the wild-type in tomato, indicating that ScpBXv is involved in virulence. To investigate ScpBXv-associated mechanisms, comparative proteomic analyses were carried out and the abundance of 187 proteins was altered. Among them, diverse transcriptional regulators involved in biofilm formation and virulence were abundant in the wild-type. We further showed that biofilm formation of XcvΔscpBXv was reduced. This study provides new insights into the functions of ScpBXv in bacterial replication and biofilm formation, which may contribute to the virulence of Xcv.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720608proteomicsScpBXanthomonas |
| spellingShingle | Hye-Jee Park Sang-Wook Han Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria The Plant Pathology Journal proteomics ScpB Xanthomonas |
| title | Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria |
| title_full | Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria |
| title_fullStr | Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria |
| title_full_unstemmed | Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria |
| title_short | Functional and Proteomic Analyses Reveal That ScpBXv Is Involved in Bacterial Growth, Virulence, and Biofilm Formation in Xanthomonas campestris pv. vesicatoria |
| title_sort | functional and proteomic analyses reveal that scpbxv is involved in bacterial growth virulence and biofilm formation in xanthomonas campestris pv vesicatoria |
| topic | proteomics ScpB Xanthomonas |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5720608 |
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