Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.
The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenera...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0258289 |
| _version_ | 1818841862627131392 |
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| author | Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova |
| author_facet | Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova |
| author_sort | Dzhirgala V Shamadykova |
| collection | DOAJ |
| description | The glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease. |
| first_indexed | 2024-12-19T04:32:49Z |
| format | Article |
| id | doaj.art-767fcb299b8c4227b76c2a11ba335bee |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T04:32:49Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-767fcb299b8c4227b76c2a11ba335bee2022-12-21T20:35:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610e025828910.1371/journal.pone.0258289Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells.Dzhirgala V ShamadykovaDmitry Y PanteleevNadezhda N KustEkaterina A SavchenkoEkaterina Y RybalkinaAlexander V RevishchinGalina V PavlovaThe glial cell line-derived neurotrophic factor (GDNF) is involved in the survival of dopaminergic neurons. Besides, GDNF can also induce axonal growth and creation of new functional synapses. GDNF potential is promising for translation to treat diseases associated with neuronal death: neurodegenerative disorders, ischemic stroke, and cerebral or spinal cord damages. Unproductive clinical trials of GDNF for Parkinson's disease treatment have induced to study this failure. A reason could be due to irrelevant producer cells that cannot perform the required post-translational modifications. The biological activity of recombinant mGDNF produced by E. coli have been compared with mGDNF produced by human cells HEK293. mGDNF variants were tested with PC12 cells, rat embryonic spinal ganglion cells, and SH-SY5Y human neuroblastoma cells in vitro as well as with a mouse model of the Parkinson's disease in vivo. Both in vitro and in vivo the best neuro-inductive ability belongs to mGDNF produced by HEK293 cells. Keywords: GDNF, neural differentiation, bacterial and mammalian expression systems, cell cultures, model of Parkinson's disease.https://doi.org/10.1371/journal.pone.0258289 |
| spellingShingle | Dzhirgala V Shamadykova Dmitry Y Panteleev Nadezhda N Kust Ekaterina A Savchenko Ekaterina Y Rybalkina Alexander V Revishchin Galina V Pavlova Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. PLoS ONE |
| title | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. |
| title_full | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. |
| title_fullStr | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. |
| title_full_unstemmed | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. |
| title_short | Neuroinductive properties of mGDNF depend on the producer, E. Coli or human cells. |
| title_sort | neuroinductive properties of mgdnf depend on the producer e coli or human cells |
| url | https://doi.org/10.1371/journal.pone.0258289 |
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