Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein
Abstract Acute pancreatitis (AP) is one of the life‐threatening diseases of the digestive system. MicroRNA has been asserted to be a regulator of AP. This paper explored the miR‐374a‐5p expression in AP patients and investigated the efficacy of AR42J cells. In this study, 60 healthy people, 58 MAP p...
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12666 |
| _version_ | 1797808820304478208 |
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| author | Fu‐Jun Wang Xue Mei |
| author_facet | Fu‐Jun Wang Xue Mei |
| author_sort | Fu‐Jun Wang |
| collection | DOAJ |
| description | Abstract Acute pancreatitis (AP) is one of the life‐threatening diseases of the digestive system. MicroRNA has been asserted to be a regulator of AP. This paper explored the miR‐374a‐5p expression in AP patients and investigated the efficacy of AR42J cells. In this study, 60 healthy people, 58 MAP patients and 58 SAP patients were included, and the serum miR‐374a‐5p levels of the subjects were detected by RT‐qPCR technology. The pancreatitis cell model was structured by stimulating AR42J cells with cerulein. Next, cell viability and apoptosis were detected by CCK‐8 assay and flow cytometry. ELISA was used to measure the concentration of cytokines, such as TNF‐α, IL‐6, and IL‐1β. The data showed that miR‐374a‐5p was downregulated in samples from AP patients, while showing discriminative power for AP populations. Attenuated miR‐374a‐5p were negatively bound up with patients' Ranson score and APACHE II score. Besides, miR‐374a‐5p was declined in cerulein‐treated AR42J cells and forced elevation of miR‐374a‐5p was beneficial to increase cell viability, and inhibit cell apoptosis and inflammation. The present study found that miR‐374a‐5p was reduced in AP serum samples, and up‐regulated expression level of miR‐374a‐5p in cell models had a protective effect on cerulein‐induced inhibition of cell function and inflammatory response. |
| first_indexed | 2024-03-13T06:43:22Z |
| format | Article |
| id | doaj.art-768007713a0c48ba902de3ca2cf2ef6f |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-03-13T06:43:22Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-768007713a0c48ba902de3ca2cf2ef6f2023-06-08T13:32:18ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502023-06-0139661662310.1002/kjm2.12666Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by ceruleinFu‐Jun Wang0Xue Mei1Emergency Medicine Clinical Research Center, Beijing Chao‐Yang Hospital Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation Beijing ChinaEmergency Medicine Clinical Research Center, Beijing Chao‐Yang Hospital Capital Medical University, Beijing Key Laboratory of Cardiopulmonary Cerebral Resuscitation Beijing ChinaAbstract Acute pancreatitis (AP) is one of the life‐threatening diseases of the digestive system. MicroRNA has been asserted to be a regulator of AP. This paper explored the miR‐374a‐5p expression in AP patients and investigated the efficacy of AR42J cells. In this study, 60 healthy people, 58 MAP patients and 58 SAP patients were included, and the serum miR‐374a‐5p levels of the subjects were detected by RT‐qPCR technology. The pancreatitis cell model was structured by stimulating AR42J cells with cerulein. Next, cell viability and apoptosis were detected by CCK‐8 assay and flow cytometry. ELISA was used to measure the concentration of cytokines, such as TNF‐α, IL‐6, and IL‐1β. The data showed that miR‐374a‐5p was downregulated in samples from AP patients, while showing discriminative power for AP populations. Attenuated miR‐374a‐5p were negatively bound up with patients' Ranson score and APACHE II score. Besides, miR‐374a‐5p was declined in cerulein‐treated AR42J cells and forced elevation of miR‐374a‐5p was beneficial to increase cell viability, and inhibit cell apoptosis and inflammation. The present study found that miR‐374a‐5p was reduced in AP serum samples, and up‐regulated expression level of miR‐374a‐5p in cell models had a protective effect on cerulein‐induced inhibition of cell function and inflammatory response.https://doi.org/10.1002/kjm2.12666acute pancreatitisdiagnosisinflammationmiR‐374a‐5p |
| spellingShingle | Fu‐Jun Wang Xue Mei Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein Kaohsiung Journal of Medical Sciences acute pancreatitis diagnosis inflammation miR‐374a‐5p |
| title | Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein |
| title_full | Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein |
| title_fullStr | Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein |
| title_full_unstemmed | Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein |
| title_short | Expression level of serum miR‐374a‐5p in patients with acute pancreatitis and its effect on viability, apoptosis, and inflammatory factors of pancreatic acinar cells induced by cerulein |
| title_sort | expression level of serum mir 374a 5p in patients with acute pancreatitis and its effect on viability apoptosis and inflammatory factors of pancreatic acinar cells induced by cerulein |
| topic | acute pancreatitis diagnosis inflammation miR‐374a‐5p |
| url | https://doi.org/10.1002/kjm2.12666 |
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