Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS
Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/ta...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.954692/full |
| _version_ | 1811265319551893504 |
|---|---|
| author | Yameng Zhu Shujie Wei Xiunan Cao Songrui Wang Yanxu Chang Huizi Ouyang Jun He |
| author_facet | Yameng Zhu Shujie Wei Xiunan Cao Songrui Wang Yanxu Chang Huizi Ouyang Jun He |
| author_sort | Yameng Zhu |
| collection | DOAJ |
| description | Prunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 16 prototype components (L-(-)-malic acid, 3,4-dihydroxybenzaldehyde, protocatechuic acid, vanillic acid, caffeic acid, D-(-)-quinic acid, citric acid, ferulic acid, syringic acid, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, amygdalin, maslinic acid, corosolic acid, and rutin) in rat plasma after oral administration of the MF extract. Plasma samples were prepared via protein precipitation using acetonitrile. The 16 components were separated on an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a gradient mobile phase system of methanol and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 ml/min. All components were quantitated using Agilent Jet Stream electrospray ionisation in negative ion mode. The intra-day and inter-day accuracies ranged from-9.4 to 9.4%, and the precision of the analytes was less than 14.8%. The extraction recovery rate of the analytes ranged from 63.59 to 109.44% and the matrix effects ranged from 49.25 to 109.28%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 13.7%. Hence, the developed method was successfully applied to evaluate the pharmacokinetics of 16 components in the MF extract after oral administration in rats using UPLC-MS/MS. |
| first_indexed | 2024-04-12T20:20:41Z |
| format | Article |
| id | doaj.art-768096e0eced4c12884c0a8d108a976b |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T20:20:41Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-768096e0eced4c12884c0a8d108a976b2022-12-22T03:18:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-09-011310.3389/fphar.2022.954692954692Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MSYameng ZhuShujie WeiXiunan CaoSongrui WangYanxu ChangHuizi OuyangJun HePrunus mume fructus (MF) is used in traditional Chinese medicine and food, as it exerts pharmacological effects, such as antibacterial, antioxidant, antitumour, thirst-relieving, and antidiarrheal effects. In the present study, a reliable and sensitive ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of 16 prototype components (L-(-)-malic acid, 3,4-dihydroxybenzaldehyde, protocatechuic acid, vanillic acid, caffeic acid, D-(-)-quinic acid, citric acid, ferulic acid, syringic acid, cryptochlorogenic acid, neochlorogenic acid, chlorogenic acid, amygdalin, maslinic acid, corosolic acid, and rutin) in rat plasma after oral administration of the MF extract. Plasma samples were prepared via protein precipitation using acetonitrile. The 16 components were separated on an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with a gradient mobile phase system of methanol and 0.1% (v/v) formic acid aqueous solution at a flow rate of 0.3 ml/min. All components were quantitated using Agilent Jet Stream electrospray ionisation in negative ion mode. The intra-day and inter-day accuracies ranged from-9.4 to 9.4%, and the precision of the analytes was less than 14.8%. The extraction recovery rate of the analytes ranged from 63.59 to 109.44% and the matrix effects ranged from 49.25 to 109.28%. Stability studies proved that the analytes were stable under the tested conditions, with a relative standard deviation lower than 13.7%. Hence, the developed method was successfully applied to evaluate the pharmacokinetics of 16 components in the MF extract after oral administration in rats using UPLC-MS/MS.https://www.frontiersin.org/articles/10.3389/fphar.2022.954692/fullPrunus mume fructus extractprototype componentspharmacokineticrat plasmaUPLC-MS/MS |
| spellingShingle | Yameng Zhu Shujie Wei Xiunan Cao Songrui Wang Yanxu Chang Huizi Ouyang Jun He Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS Frontiers in Pharmacology Prunus mume fructus extract prototype components pharmacokinetic rat plasma UPLC-MS/MS |
| title | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
| title_full | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
| title_fullStr | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
| title_full_unstemmed | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
| title_short | Multi-component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using UPLC-MS/MS |
| title_sort | multi component pharmacokinetic study of prunus mume fructus extract after oral administration in rats using uplc ms ms |
| topic | Prunus mume fructus extract prototype components pharmacokinetic rat plasma UPLC-MS/MS |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.954692/full |
| work_keys_str_mv | AT yamengzhu multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT shujiewei multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT xiunancao multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT songruiwang multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT yanxuchang multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT huiziouyang multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms AT junhe multicomponentpharmacokineticstudyofprunusmumefructusextractafteroraladministrationinratsusinguplcmsms |