Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line
Background and purpose: Nowadays, multi-drug regimens or combination therapy are new approaches in cancer treatment that combine two or several therapeutic agents. Combination therapy aims at improving the therapeutic efficacy of anticancer drugs while reducing toxicity and preventing drug resistanc...
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| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2022-10-01
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| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-18070-en.html |
| _version_ | 1828002809845383168 |
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| author | Mohammad Shokrzadeh Fatemeh Shaki Nahid Amani |
| author_facet | Mohammad Shokrzadeh Fatemeh Shaki Nahid Amani |
| author_sort | Mohammad Shokrzadeh |
| collection | DOAJ |
| description | Background and purpose: Nowadays, multi-drug regimens or combination therapy are new approaches in cancer treatment that combine two or several therapeutic agents. Combination therapy aims at improving the therapeutic efficacy of anticancer drugs while reducing toxicity and preventing drug resistance. The aim of this study was to determine the synergistic effect of combined clarithromycin and doxorubicin in lung cancer A549 cell line.
Materials and methods: Combined cytotoxicity of doxorubicin and clarithromycin was evaluated by MTT assay in A549 cell line. The nature of the interaction observed between doxorubicin and clarithromycin was analyzed in Compusyn software based on Chou and Talalay method. In order to clarify the molecular mechanism of the drug synergism, apoptosis was measured by Annexin V/PI double staining.
Results: Clarithromycin at 500 µM synergistically reduced the IC50 value of doxorubicin. The enhanced cytotoxicity of doxorubicin by clarithromycin was associated with increased apoptotic cell death in A549 cell line.
Conclusion: Clarithromycin is a suitable candidate when combined with other anticancer drugs in vitro. However, more investigations are needed to evaluate the application of clarithromycin in adjuvant cancer therapy. |
| first_indexed | 2024-04-10T06:49:32Z |
| format | Article |
| id | doaj.art-76834eea2f524a7fa1fc9217df108d96 |
| institution | Directory Open Access Journal |
| issn | 1735-9260 1735-9279 |
| language | English |
| last_indexed | 2024-04-10T06:49:32Z |
| publishDate | 2022-10-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj.art-76834eea2f524a7fa1fc9217df108d962023-02-28T09:08:45ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792022-10-01322134254Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell LineMohammad Shokrzadeh0Fatemeh Shaki1Nahid Amani2 Professor, Department of Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Associate Professor, Pharmaceutical Sciences Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran . Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran PhD in Toxicology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran Background and purpose: Nowadays, multi-drug regimens or combination therapy are new approaches in cancer treatment that combine two or several therapeutic agents. Combination therapy aims at improving the therapeutic efficacy of anticancer drugs while reducing toxicity and preventing drug resistance. The aim of this study was to determine the synergistic effect of combined clarithromycin and doxorubicin in lung cancer A549 cell line. Materials and methods: Combined cytotoxicity of doxorubicin and clarithromycin was evaluated by MTT assay in A549 cell line. The nature of the interaction observed between doxorubicin and clarithromycin was analyzed in Compusyn software based on Chou and Talalay method. In order to clarify the molecular mechanism of the drug synergism, apoptosis was measured by Annexin V/PI double staining. Results: Clarithromycin at 500 µM synergistically reduced the IC50 value of doxorubicin. The enhanced cytotoxicity of doxorubicin by clarithromycin was associated with increased apoptotic cell death in A549 cell line. Conclusion: Clarithromycin is a suitable candidate when combined with other anticancer drugs in vitro. However, more investigations are needed to evaluate the application of clarithromycin in adjuvant cancer therapy.http://jmums.mazums.ac.ir/article-1-18070-en.htmldoxorubicinclarithromycincombination therapysynergismapoptosis |
| spellingShingle | Mohammad Shokrzadeh Fatemeh Shaki Nahid Amani Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line Journal of Mazandaran University of Medical Sciences doxorubicin clarithromycin combination therapy synergism apoptosis |
| title | Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line |
| title_full | Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line |
| title_fullStr | Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line |
| title_full_unstemmed | Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line |
| title_short | Effect of Clarithromycin on Enhancement of Doxorubicin-induced Cytotoxicity and Apoptosis in A549 Tumor-Cell Line |
| title_sort | effect of clarithromycin on enhancement of doxorubicin induced cytotoxicity and apoptosis in a549 tumor cell line |
| topic | doxorubicin clarithromycin combination therapy synergism apoptosis |
| url | http://jmums.mazums.ac.ir/article-1-18070-en.html |
| work_keys_str_mv | AT mohammadshokrzadeh effectofclarithromycinonenhancementofdoxorubicininducedcytotoxicityandapoptosisina549tumorcellline AT fatemehshaki effectofclarithromycinonenhancementofdoxorubicininducedcytotoxicityandapoptosisina549tumorcellline AT nahidamani effectofclarithromycinonenhancementofdoxorubicininducedcytotoxicityandapoptosisina549tumorcellline |