In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells
Abstract Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is di...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-45285-0 |
| _version_ | 1797273890997665792 |
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| author | Yusuke Seto Ryoma Ogihara Kaori Takizawa Mototsugu Eiraku |
| author_facet | Yusuke Seto Ryoma Ogihara Kaori Takizawa Mototsugu Eiraku |
| author_sort | Yusuke Seto |
| collection | DOAJ |
| description | Abstract Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is difficult. In this study, we develop a simplified in vitro 3D model using human pluripotent stem cells to analyze the early stages of facial development. In this model, cranial NCC-like cells spontaneously differentiate from neural plate border-like cells into maxillary arch-like mesenchyme after a long-term culture. Upon the addition of EDN1 and BMP4, these aggregates are converted into a mandibular arch-like state. Furthermore, temporary treatment with EDN1 and BMP4 induces the formation of spatially separated domains expressing mandibular and maxillary arch markers within a single aggregate. These results suggest that this in vitro model is useful for determining the mechanisms underlying cell fate specification and patterning during early facial development. |
| first_indexed | 2024-03-07T14:50:36Z |
| format | Article |
| id | doaj.art-76839873891f4026b9124d55fadb1d5d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-07T14:50:36Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-76839873891f4026b9124d55fadb1d5d2024-03-05T19:43:41ZengNature PortfolioNature Communications2041-17232024-02-0115111710.1038/s41467-024-45285-0In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cellsYusuke Seto0Ryoma Ogihara1Kaori Takizawa2Mototsugu Eiraku3Laboratory of Developmental Systems, Institute for Life and Medical Sciences, Kyoto UniversityDepartment of Polymer Chemistry, Graduate School of Engineering, Kyoto UniversityLaboratory of Developmental Systems, Institute for Life and Medical Sciences, Kyoto UniversityLaboratory of Developmental Systems, Institute for Life and Medical Sciences, Kyoto UniversityAbstract Early patterning of neural crest cells (NCCs) in the craniofacial primordium is important for subsequent development of proper craniofacial structures. However, because of the complexity of the environment of developing tissues, surveying the early specification and patterning of NCCs is difficult. In this study, we develop a simplified in vitro 3D model using human pluripotent stem cells to analyze the early stages of facial development. In this model, cranial NCC-like cells spontaneously differentiate from neural plate border-like cells into maxillary arch-like mesenchyme after a long-term culture. Upon the addition of EDN1 and BMP4, these aggregates are converted into a mandibular arch-like state. Furthermore, temporary treatment with EDN1 and BMP4 induces the formation of spatially separated domains expressing mandibular and maxillary arch markers within a single aggregate. These results suggest that this in vitro model is useful for determining the mechanisms underlying cell fate specification and patterning during early facial development.https://doi.org/10.1038/s41467-024-45285-0 |
| spellingShingle | Yusuke Seto Ryoma Ogihara Kaori Takizawa Mototsugu Eiraku In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells Nature Communications |
| title | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
| title_full | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
| title_fullStr | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
| title_full_unstemmed | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
| title_short | In vitro induction of patterned branchial arch-like aggregate from human pluripotent stem cells |
| title_sort | in vitro induction of patterned branchial arch like aggregate from human pluripotent stem cells |
| url | https://doi.org/10.1038/s41467-024-45285-0 |
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