BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186
Bcl2-associated athanogene (BAG)2 as a co-chaperone has been demonstrated to be involved in tumor growth and metastasis, but its biological function in gastric cancer remains unknown. Here, we reported that BAG2 was highly expressed in gastric cancer cell lines and tissues, indicating poor prognosis...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-01-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00031/full |
| _version_ | 1828775296753467392 |
|---|---|
| author | Lisha Sun Guanglei Chen Anqi Sun Zheng Wang Haibo Huang Ziming Gao Weitian Liang Caigang Liu Kai Li |
| author_facet | Lisha Sun Guanglei Chen Anqi Sun Zheng Wang Haibo Huang Ziming Gao Weitian Liang Caigang Liu Kai Li |
| author_sort | Lisha Sun |
| collection | DOAJ |
| description | Bcl2-associated athanogene (BAG)2 as a co-chaperone has been demonstrated to be involved in tumor growth and metastasis, but its biological function in gastric cancer remains unknown. Here, we reported that BAG2 was highly expressed in gastric cancer cell lines and tissues, indicating poor prognosis. High expression of BAG2 was significantly associated with T stage and differentiation level of gastric cancer (P < 0.001). Functional experiments revealed that BAG2 knockdown in gastric cancer cells inhibited the proliferation, invasion and migration of cells through AKT/mTOR and extracellular regulated kinase (ERK) pathways. Proteomic analysis identified that BAG2 may be involved in the regulation of mitogen-activated protein kinase (MAPK) pathway. In addition, immunoprecipitation showed that BAG2 could bind to ERK1/2. Luciferase reporter assay and Western blot verified that BAG2 was down-regulated by miR186. Taken together, our findings may reveal the basic function of BAG2 and uncover a potential therapeutic target for gastric cancer. |
| first_indexed | 2024-12-11T15:36:43Z |
| format | Article |
| id | doaj.art-7685a1d5a22c4e49b6f0c12c02f4af8c |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-11T15:36:43Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-7685a1d5a22c4e49b6f0c12c02f4af8c2022-12-22T00:59:54ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-01-011010.3389/fonc.2020.00031474097BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186Lisha Sun0Guanglei Chen1Anqi Sun2Zheng Wang3Haibo Huang4Ziming Gao5Weitian Liang6Caigang Liu7Kai Li8Department of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Otorhinolaryngology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaDepartment of Breast Surgery, Shengjing Hospital of China Medical University, Shenyang, ChinaDepartment of Surgical Oncology, The First Hospital of China Medical University, Shenyang, ChinaBcl2-associated athanogene (BAG)2 as a co-chaperone has been demonstrated to be involved in tumor growth and metastasis, but its biological function in gastric cancer remains unknown. Here, we reported that BAG2 was highly expressed in gastric cancer cell lines and tissues, indicating poor prognosis. High expression of BAG2 was significantly associated with T stage and differentiation level of gastric cancer (P < 0.001). Functional experiments revealed that BAG2 knockdown in gastric cancer cells inhibited the proliferation, invasion and migration of cells through AKT/mTOR and extracellular regulated kinase (ERK) pathways. Proteomic analysis identified that BAG2 may be involved in the regulation of mitogen-activated protein kinase (MAPK) pathway. In addition, immunoprecipitation showed that BAG2 could bind to ERK1/2. Luciferase reporter assay and Western blot verified that BAG2 was down-regulated by miR186. Taken together, our findings may reveal the basic function of BAG2 and uncover a potential therapeutic target for gastric cancer.https://www.frontiersin.org/article/10.3389/fonc.2020.00031/fullgastric cancerBAG2miR186iTRAQ proteomics technologytherapeutic target |
| spellingShingle | Lisha Sun Guanglei Chen Anqi Sun Zheng Wang Haibo Huang Ziming Gao Weitian Liang Caigang Liu Kai Li BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 Frontiers in Oncology gastric cancer BAG2 miR186 iTRAQ proteomics technology therapeutic target |
| title | BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 |
| title_full | BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 |
| title_fullStr | BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 |
| title_full_unstemmed | BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 |
| title_short | BAG2 Promotes Proliferation and Metastasis of Gastric Cancer via ERK1/2 Signaling and Partially Regulated by miR186 |
| title_sort | bag2 promotes proliferation and metastasis of gastric cancer via erk1 2 signaling and partially regulated by mir186 |
| topic | gastric cancer BAG2 miR186 iTRAQ proteomics technology therapeutic target |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00031/full |
| work_keys_str_mv | AT lishasun bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT guangleichen bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT anqisun bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT zhengwang bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT haibohuang bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT ziminggao bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT weitianliang bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT caigangliu bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 AT kaili bag2promotesproliferationandmetastasisofgastriccancerviaerk12signalingandpartiallyregulatedbymir186 |