Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method
In different countries, interest in the commercial cultivation of the olive has recently greatly increased, which has led to the expansion of its range. The Crimean Peninsula is the northern limit of the common olive (<i>Olea europaea</i> L.) range. A unique collection of common olive’s...
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MDPI AG
2023-06-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/7/1323 |
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| author | Natalia Slobodova Fedor Sharko Maria Gladysheva-Azgari Kristina Petrova Sergey Tsiupka Valentina Tsiupka Eugenia Boulygina Sergey Rastorguev Svetlana Tsygankova |
| author_facet | Natalia Slobodova Fedor Sharko Maria Gladysheva-Azgari Kristina Petrova Sergey Tsiupka Valentina Tsiupka Eugenia Boulygina Sergey Rastorguev Svetlana Tsygankova |
| author_sort | Natalia Slobodova |
| collection | DOAJ |
| description | In different countries, interest in the commercial cultivation of the olive has recently greatly increased, which has led to the expansion of its range. The Crimean Peninsula is the northern limit of the common olive (<i>Olea europaea</i> L.) range. A unique collection of common olive’s cultivars and hybrids has been collected in the Nikitsky Botanical Gardens (NBG). The aim of this study was to assess the genetic diversity of 151 samples (total of several biological replicates of 46 olive cultivars including 29 introduced and 11 indigenous genotypes) using the ddRAD sequencing method. Structural analysis showed that the studied samples are divided into ten groups, each of which mainly includes cultivars of the same origin. Cultivars introduced to the Crimean Peninsula from different regions formed separate groups, while local cultivars joined different groups depending on their origin. Cultivars of Crimean origin contain admixtures of mainly Italian and Caucasian cultivars’ genotypes. Our study showed that the significant number of Crimean cultivars contains an admixture of the Italian cultivar “Coreggiolo”. Genetic analysis confirmed the synonymy for the cv. “Otur” and “Nikitskaya 2”, but not for the other four putative synonyms. Our results revealed the genetic diversity of the olive collection of NBG and provided references for future research studies, especially in selection studies for breeding programs. |
| first_indexed | 2024-03-11T01:02:42Z |
| format | Article |
| id | doaj.art-76893a60dfee4cec980c9b276454c5f7 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T01:02:42Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-76893a60dfee4cec980c9b276454c5f72023-11-18T19:28:44ZengMDPI AGGenes2073-44252023-06-01147132310.3390/genes14071323Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq MethodNatalia Slobodova0Fedor Sharko1Maria Gladysheva-Azgari2Kristina Petrova3Sergey Tsiupka4Valentina Tsiupka5Eugenia Boulygina6Sergey Rastorguev7Svetlana Tsygankova8National Research Center “Kurchatov Institute”, Moscow 123182, RussiaNational Research Center “Kurchatov Institute”, Moscow 123182, RussiaNational Research Center “Kurchatov Institute”, Moscow 123182, RussiaResearch Center for Medical Genetics, Moscow 115522, RussiaNikita Botanical Gardens–National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, RussiaNikita Botanical Gardens–National Scientific Centre of the Russian Academy of Sciences, Yalta 298648, RussiaNational Research Center “Kurchatov Institute”, Moscow 123182, RussiaPirogov Russian National Research Medical University, Moscow 117997, RussiaNational Research Center “Kurchatov Institute”, Moscow 123182, RussiaIn different countries, interest in the commercial cultivation of the olive has recently greatly increased, which has led to the expansion of its range. The Crimean Peninsula is the northern limit of the common olive (<i>Olea europaea</i> L.) range. A unique collection of common olive’s cultivars and hybrids has been collected in the Nikitsky Botanical Gardens (NBG). The aim of this study was to assess the genetic diversity of 151 samples (total of several biological replicates of 46 olive cultivars including 29 introduced and 11 indigenous genotypes) using the ddRAD sequencing method. Structural analysis showed that the studied samples are divided into ten groups, each of which mainly includes cultivars of the same origin. Cultivars introduced to the Crimean Peninsula from different regions formed separate groups, while local cultivars joined different groups depending on their origin. Cultivars of Crimean origin contain admixtures of mainly Italian and Caucasian cultivars’ genotypes. Our study showed that the significant number of Crimean cultivars contains an admixture of the Italian cultivar “Coreggiolo”. Genetic analysis confirmed the synonymy for the cv. “Otur” and “Nikitskaya 2”, but not for the other four putative synonyms. Our results revealed the genetic diversity of the olive collection of NBG and provided references for future research studies, especially in selection studies for breeding programs.https://www.mdpi.com/2073-4425/14/7/1323<i>Olea europaea</i> L. (olive tree)ddRAD sequencinggeographical distribution of cultivarsfixation indexidentity by state |
| spellingShingle | Natalia Slobodova Fedor Sharko Maria Gladysheva-Azgari Kristina Petrova Sergey Tsiupka Valentina Tsiupka Eugenia Boulygina Sergey Rastorguev Svetlana Tsygankova Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method Genes <i>Olea europaea</i> L. (olive tree) ddRAD sequencing geographical distribution of cultivars fixation index identity by state |
| title | Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method |
| title_full | Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method |
| title_fullStr | Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method |
| title_full_unstemmed | Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method |
| title_short | Genetic Diversity of Common Olive (<i>Olea europaea</i> L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method |
| title_sort | genetic diversity of common olive i olea europaea i l cultivars from nikita botanical gardens collection revealed using rad seq method |
| topic | <i>Olea europaea</i> L. (olive tree) ddRAD sequencing geographical distribution of cultivars fixation index identity by state |
| url | https://www.mdpi.com/2073-4425/14/7/1323 |
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