Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes
We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, featu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422000795 |
| _version_ | 1828292329869410304 |
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| author | Chenwei Xiong Jie Li Luyao Li Long Chen Rong Zhang Xianqiang Mi Yifan Liu |
| author_facet | Chenwei Xiong Jie Li Luyao Li Long Chen Rong Zhang Xianqiang Mi Yifan Liu |
| author_sort | Chenwei Xiong |
| collection | DOAJ |
| description | We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, features high cost-effectiveness and convenient fabrication. This is realized by patterning a micrometer-sized heterojunction consisting of adjacent segments of polycationic and polyanionic hydrogels on a microfluidic chip through simple photocuring steps. The integrated diode rectifies ionic currents being sensitive to the charge of DNA adsorbed onto the polycationic chains through electrostatic associations. Based on the mechanism, we show that the ionic biosensor can electrically quantify DNA in a dynamic range relevant to typical nucleic acid amplification assays. Utilizing the device, we demonstrate the evaluation of a PCR assay amplifying a 500-bp DNA fragment of E. coli, an infection-causing pathogen, and real-time in situ monitoring of an isothermal assay amplifying E. coli whole genome. We anticipate that the device could potentially pave the way for miniaturized optics-free platforms for quantifying nucleic acid amplification at point-of-care. |
| first_indexed | 2024-04-13T11:06:35Z |
| format | Article |
| id | doaj.art-76896434043e4cc289f8561f5183746c |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-13T11:06:35Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-76896434043e4cc289f8561f5183746c2022-12-22T02:49:15ZengElsevierMaterials Today Bio2590-00642022-06-0115100281Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodesChenwei Xiong0Jie Li1Luyao Li2Long Chen3Rong Zhang4Xianqiang Mi5Yifan Liu6Division of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China; University of Chinese Academy of Sciences, Beijing, 100049, ChinaDivision of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, ChinaShanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China; University of Chinese Academy of Sciences, Beijing, 100049, China; Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China; Key Laboratory of Systems Biology, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Hangzhou, 310024, China; Key Laboratory of Systems Health Science of Zhejiang Province, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; Corresponding author. Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, 200050, China.Division of Chemistry and Physical Biology, School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Corresponding author.We demonstrate here for the first time the utility of a monolithically integrated hydrogel ionic diode for label-free quantitative DNA detection and real-time monitoring of nucleic acid amplification. The hydrogel ionic diode presented herein, unlike nanomaterial-based field-effect biosensors, features high cost-effectiveness and convenient fabrication. This is realized by patterning a micrometer-sized heterojunction consisting of adjacent segments of polycationic and polyanionic hydrogels on a microfluidic chip through simple photocuring steps. The integrated diode rectifies ionic currents being sensitive to the charge of DNA adsorbed onto the polycationic chains through electrostatic associations. Based on the mechanism, we show that the ionic biosensor can electrically quantify DNA in a dynamic range relevant to typical nucleic acid amplification assays. Utilizing the device, we demonstrate the evaluation of a PCR assay amplifying a 500-bp DNA fragment of E. coli, an infection-causing pathogen, and real-time in situ monitoring of an isothermal assay amplifying E. coli whole genome. We anticipate that the device could potentially pave the way for miniaturized optics-free platforms for quantifying nucleic acid amplification at point-of-care.http://www.sciencedirect.com/science/article/pii/S2590006422000795HydrogelIonic diodeElectrical biosensorNucleic acidPoint-of-care |
| spellingShingle | Chenwei Xiong Jie Li Luyao Li Long Chen Rong Zhang Xianqiang Mi Yifan Liu Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes Materials Today Bio Hydrogel Ionic diode Electrical biosensor Nucleic acid Point-of-care |
| title | Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| title_full | Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| title_fullStr | Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| title_full_unstemmed | Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| title_short | Label-free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| title_sort | label free electrical monitoring of nucleic acid amplification with integrated hydrogel ionic diodes |
| topic | Hydrogel Ionic diode Electrical biosensor Nucleic acid Point-of-care |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422000795 |
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