The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians
Lamprey homologues of the classic embryonic inducer Noggin are similar in expression pattern and functional properties to Noggin homologues of jawed vertebrates. All <i>noggin</i> genes of vertebrates apparently originated from a single ancestral gene as a result of genome duplications....
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/2412 |
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| author | Galina V. Ermakova Aleksandr V. Kucheryavyy Andrey G. Zaraisky Andrey V. Bayramov |
| author_facet | Galina V. Ermakova Aleksandr V. Kucheryavyy Andrey G. Zaraisky Andrey V. Bayramov |
| author_sort | Galina V. Ermakova |
| collection | DOAJ |
| description | Lamprey homologues of the classic embryonic inducer Noggin are similar in expression pattern and functional properties to Noggin homologues of jawed vertebrates. All <i>noggin</i> genes of vertebrates apparently originated from a single ancestral gene as a result of genome duplications. <i>nogginA</i>, <i>nogginB</i> and <i>nogginC</i> of lampreys, like <i>noggin1</i> and <i>noggin2</i> of gnathostomes, demonstrate the ability to induce complete secondary axes with forebrain and eye structures when overexpressed in <i>Xenopus laevis</i> embryos. According to current views, this finding indicates the ability of lamprey Noggin proteins to suppress the activity of the BMP, Nodal/Activin and Wnt/beta-catenin signaling pathways, as shown for Noggin proteins of gnathostomes. In this work, by analogy with experiments in <i>Xenopus</i> embryos, we attempted to induce secondary axes in the European river lamprey <i>Lampetra fluviatilis</i> by injecting <i>noggin</i> mRNAs into lamprey eggs in vivo. Surprisingly, unlike what occurs in amphibians, secondary axis induction in the lampreys either by <i>noggin</i> mRNAs or by <i>chordin</i> and <i>cerberus</i> mRNAs, the inductive properties of which have been described, was not observed. Only <i>wnt8a</i> mRNA demonstrated the ability to induce secondary axes in the lampreys. Such results may indicate that the mechanism of axial specification in lampreys, which represent jawless vertebrates, may differ in detail from that in the jawed clade. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:28:41Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-768c11d904ac4ae1b340ef2f55efa9602024-02-23T15:20:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254241210.3390/ijms25042412The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in AmphibiansGalina V. Ermakova0Aleksandr V. Kucheryavyy1Andrey G. Zaraisky2Andrey V. Bayramov3Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, RussiaSevertsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow 119071, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, RussiaLamprey homologues of the classic embryonic inducer Noggin are similar in expression pattern and functional properties to Noggin homologues of jawed vertebrates. All <i>noggin</i> genes of vertebrates apparently originated from a single ancestral gene as a result of genome duplications. <i>nogginA</i>, <i>nogginB</i> and <i>nogginC</i> of lampreys, like <i>noggin1</i> and <i>noggin2</i> of gnathostomes, demonstrate the ability to induce complete secondary axes with forebrain and eye structures when overexpressed in <i>Xenopus laevis</i> embryos. According to current views, this finding indicates the ability of lamprey Noggin proteins to suppress the activity of the BMP, Nodal/Activin and Wnt/beta-catenin signaling pathways, as shown for Noggin proteins of gnathostomes. In this work, by analogy with experiments in <i>Xenopus</i> embryos, we attempted to induce secondary axes in the European river lamprey <i>Lampetra fluviatilis</i> by injecting <i>noggin</i> mRNAs into lamprey eggs in vivo. Surprisingly, unlike what occurs in amphibians, secondary axis induction in the lampreys either by <i>noggin</i> mRNAs or by <i>chordin</i> and <i>cerberus</i> mRNAs, the inductive properties of which have been described, was not observed. Only <i>wnt8a</i> mRNA demonstrated the ability to induce secondary axes in the lampreys. Such results may indicate that the mechanism of axial specification in lampreys, which represent jawless vertebrates, may differ in detail from that in the jawed clade.https://www.mdpi.com/1422-0067/25/4/2412embryonic inductionlampreyNogginChordinaxis development |
| spellingShingle | Galina V. Ermakova Aleksandr V. Kucheryavyy Andrey G. Zaraisky Andrey V. Bayramov The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians International Journal of Molecular Sciences embryonic induction lamprey Noggin Chordin axis development |
| title | The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians |
| title_full | The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians |
| title_fullStr | The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians |
| title_full_unstemmed | The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians |
| title_short | The Molecular Mechanism of Body Axis Induction in Lampreys May Differ from That in Amphibians |
| title_sort | molecular mechanism of body axis induction in lampreys may differ from that in amphibians |
| topic | embryonic induction lamprey Noggin Chordin axis development |
| url | https://www.mdpi.com/1422-0067/25/4/2412 |
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