Micelles as delivery vehicles for oligofluorene for bioimaging.
With the successful development of organic/polymeric light emitting diodes, many organic and polymeric fluorophores with high quantum efficiencies and optical stability were synthesized. However, most of these materials which have excellent optical properties are insoluble in water, limiting their a...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3167853?pdf=render |
| _version_ | 1818036670699667456 |
|---|---|
| author | Fengyu Su Ruhaniyah Alam Qian Mei Yanqing Tian Deirdre R Meldrum |
| author_facet | Fengyu Su Ruhaniyah Alam Qian Mei Yanqing Tian Deirdre R Meldrum |
| author_sort | Fengyu Su |
| collection | DOAJ |
| description | With the successful development of organic/polymeric light emitting diodes, many organic and polymeric fluorophores with high quantum efficiencies and optical stability were synthesized. However, most of these materials which have excellent optical properties are insoluble in water, limiting their applications in biological fields. Herein, we used micelles formed from an amino-group-containing poly(ε-caprolactone)-block-poly(ethylene glycol) (PCL-b-PEG-NH(2)) to incorporate a hydrophobic blue emitter oligofluorene (OF) to enable its application in biological conditions. Although OF is completely insoluble in water, it was successfully transferred into aqueous solutions with a good retention of its photophysical properties. OF exhibited a high quantum efficiency of 0.84 in a typical organic solvent of tetrahydrofuran (THF). In addition, OF also showed a good quantum efficiency of 0.46 after being encapsulated into micelles. Two cells lines, human glioblastoma (U87MG) and esophagus premalignant (CP-A), were used to study the cellular internalization of the OF incorporated micelles. Results showed that the hydrophobic OF was located in the cytoplasm, which was confirmed by co-staining the cells with nucleic acid specific SYTO 9, lysosome specific LysoTracker Red®, and mitochondria specific MitoTracker Red. MTT assay indicated non-toxicity of the OF-incorporated micelles. This study will broaden the application of hydrophobic functional organic compounds, oligomers, and polymers with good optical properties to enable their applications in biological research fields. |
| first_indexed | 2024-12-10T07:14:39Z |
| format | Article |
| id | doaj.art-768ea3a10a40413ca911c0287a5062fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:14:39Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-768ea3a10a40413ca911c0287a5062fc2022-12-22T01:57:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0169e2442510.1371/journal.pone.0024425Micelles as delivery vehicles for oligofluorene for bioimaging.Fengyu SuRuhaniyah AlamQian MeiYanqing TianDeirdre R MeldrumWith the successful development of organic/polymeric light emitting diodes, many organic and polymeric fluorophores with high quantum efficiencies and optical stability were synthesized. However, most of these materials which have excellent optical properties are insoluble in water, limiting their applications in biological fields. Herein, we used micelles formed from an amino-group-containing poly(ε-caprolactone)-block-poly(ethylene glycol) (PCL-b-PEG-NH(2)) to incorporate a hydrophobic blue emitter oligofluorene (OF) to enable its application in biological conditions. Although OF is completely insoluble in water, it was successfully transferred into aqueous solutions with a good retention of its photophysical properties. OF exhibited a high quantum efficiency of 0.84 in a typical organic solvent of tetrahydrofuran (THF). In addition, OF also showed a good quantum efficiency of 0.46 after being encapsulated into micelles. Two cells lines, human glioblastoma (U87MG) and esophagus premalignant (CP-A), were used to study the cellular internalization of the OF incorporated micelles. Results showed that the hydrophobic OF was located in the cytoplasm, which was confirmed by co-staining the cells with nucleic acid specific SYTO 9, lysosome specific LysoTracker Red®, and mitochondria specific MitoTracker Red. MTT assay indicated non-toxicity of the OF-incorporated micelles. This study will broaden the application of hydrophobic functional organic compounds, oligomers, and polymers with good optical properties to enable their applications in biological research fields.http://europepmc.org/articles/PMC3167853?pdf=render |
| spellingShingle | Fengyu Su Ruhaniyah Alam Qian Mei Yanqing Tian Deirdre R Meldrum Micelles as delivery vehicles for oligofluorene for bioimaging. PLoS ONE |
| title | Micelles as delivery vehicles for oligofluorene for bioimaging. |
| title_full | Micelles as delivery vehicles for oligofluorene for bioimaging. |
| title_fullStr | Micelles as delivery vehicles for oligofluorene for bioimaging. |
| title_full_unstemmed | Micelles as delivery vehicles for oligofluorene for bioimaging. |
| title_short | Micelles as delivery vehicles for oligofluorene for bioimaging. |
| title_sort | micelles as delivery vehicles for oligofluorene for bioimaging |
| url | http://europepmc.org/articles/PMC3167853?pdf=render |
| work_keys_str_mv | AT fengyusu micellesasdeliveryvehiclesforoligofluoreneforbioimaging AT ruhaniyahalam micellesasdeliveryvehiclesforoligofluoreneforbioimaging AT qianmei micellesasdeliveryvehiclesforoligofluoreneforbioimaging AT yanqingtian micellesasdeliveryvehiclesforoligofluoreneforbioimaging AT deirdrermeldrum micellesasdeliveryvehiclesforoligofluoreneforbioimaging |