The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata)
The stress response in fish is characterized by the activation of the HPI axis resulting in the release of cortisol. Previous studies in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) have shown that an acute stressor modulates the expression of immune- and stress-related...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.828050/full |
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| author | Eva Vallejos-Vidal Eva Vallejos-Vidal Eva Vallejos-Vidal Ali Reza Khansari Lluc Soliva-Dueso Joan Carles Balasch Lluis Tort Felipe E. Reyes-López Felipe E. Reyes-López |
| author_facet | Eva Vallejos-Vidal Eva Vallejos-Vidal Eva Vallejos-Vidal Ali Reza Khansari Lluc Soliva-Dueso Joan Carles Balasch Lluis Tort Felipe E. Reyes-López Felipe E. Reyes-López |
| author_sort | Eva Vallejos-Vidal |
| collection | DOAJ |
| description | The stress response in fish is characterized by the activation of the HPI axis resulting in the release of cortisol. Previous studies in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) have shown that an acute stressor modulates the expression of immune- and stress-related genes in mucosal-associated lymphoid tissues (MALTs), particularly in the skin (SALT), gills (GIALT), and gut (GALT). However, there are no antecedents on whether the modulation on the mucosal transcriptomic profile is coordinated through the local presence of cortisol in the mucosal tissue surface. Thus, the aim of this study was to evaluate the effect of cortisol upon the modulation of a set of immune- (il-1β, il-6, tnf-α, and cox-2) and stress-related (hsp70, gr1) genes. For this purpose, tissue explants cultures were incubated with cortisol (100 ng/100 mg tissue) for 2-, 4-, and 24 h and the gene expression profile was evaluated at each time-point by real-time PCR. No differences were found in the gene expression between cortisol-incubated tissue explants and mock-incubated tissues in any of the time-points tested for both species. These results suggest that the quick modulation of the gene expression during the first 24 h after the exposure to stressor challenge reported in previous studies, is probably coordinated and mediated through a systemic-dependent mechanism but not through a peripheral/local response on mucosal tissue surfaces. |
| first_indexed | 2024-12-22T06:39:28Z |
| format | Article |
| id | doaj.art-7690107768464a179db954eaf92f2fa6 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-12-22T06:39:28Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-7690107768464a179db954eaf92f2fa62022-12-21T18:35:29ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-03-01910.3389/fmars.2022.828050828050The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata)Eva Vallejos-Vidal0Eva Vallejos-Vidal1Eva Vallejos-Vidal2Ali Reza Khansari3Lluc Soliva-Dueso4Joan Carles Balasch5Lluis Tort6Felipe E. Reyes-López7Felipe E. Reyes-López8Department of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileFacultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Santiago, ChileDepartment of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainDepartment of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainDepartment of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainDepartment of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainDepartment of Cell Biology, Physiology, and Immunology, Faculty of Biosciences, Universitat Autonoma de Barcelona, Barcelona, SpainCentro de Biotecnología Acuícola, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, ChileThe stress response in fish is characterized by the activation of the HPI axis resulting in the release of cortisol. Previous studies in rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) have shown that an acute stressor modulates the expression of immune- and stress-related genes in mucosal-associated lymphoid tissues (MALTs), particularly in the skin (SALT), gills (GIALT), and gut (GALT). However, there are no antecedents on whether the modulation on the mucosal transcriptomic profile is coordinated through the local presence of cortisol in the mucosal tissue surface. Thus, the aim of this study was to evaluate the effect of cortisol upon the modulation of a set of immune- (il-1β, il-6, tnf-α, and cox-2) and stress-related (hsp70, gr1) genes. For this purpose, tissue explants cultures were incubated with cortisol (100 ng/100 mg tissue) for 2-, 4-, and 24 h and the gene expression profile was evaluated at each time-point by real-time PCR. No differences were found in the gene expression between cortisol-incubated tissue explants and mock-incubated tissues in any of the time-points tested for both species. These results suggest that the quick modulation of the gene expression during the first 24 h after the exposure to stressor challenge reported in previous studies, is probably coordinated and mediated through a systemic-dependent mechanism but not through a peripheral/local response on mucosal tissue surfaces.https://www.frontiersin.org/articles/10.3389/fmars.2022.828050/fullglucocorticoidsfish stressimmune responsegene expressionmucosal tissuesfish physiology |
| spellingShingle | Eva Vallejos-Vidal Eva Vallejos-Vidal Eva Vallejos-Vidal Ali Reza Khansari Lluc Soliva-Dueso Joan Carles Balasch Lluis Tort Felipe E. Reyes-López Felipe E. Reyes-López The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) Frontiers in Marine Science glucocorticoids fish stress immune response gene expression mucosal tissues fish physiology |
| title | The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) |
| title_full | The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) |
| title_fullStr | The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) |
| title_full_unstemmed | The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) |
| title_short | The Direct Exposure of Cortisol Does Not Modulate the Expression of Immune-Related Genes on Tissue Explants of Mucosal Surfaces in Rainbow Trout (Oncorhynchus mykiss) Nor in Gilthead Sea Bream (Sparus aurata) |
| title_sort | direct exposure of cortisol does not modulate the expression of immune related genes on tissue explants of mucosal surfaces in rainbow trout oncorhynchus mykiss nor in gilthead sea bream sparus aurata |
| topic | glucocorticoids fish stress immune response gene expression mucosal tissues fish physiology |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.828050/full |
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