Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections
Background: Escherichia coli (E. coli) is a major cause of urinary tract infection in children and women. Several virulence factors have been detected in uropathogenic Escherichia coli strains. P, S and Afa fimbria are the most important factors which are coding by pap, sfa and afa genes, respective...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | fas |
| Published: |
Isfahan University of Medical Sciences
2015-06-01
|
| Series: | مجله دانشکده پزشکی اصفهان |
| Subjects: | |
| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/4843 |
| _version_ | 1797759664523313152 |
|---|---|
| author | Mahbobeh Eslami Reza Ghanbarpour |
| author_facet | Mahbobeh Eslami Reza Ghanbarpour |
| author_sort | Mahbobeh Eslami |
| collection | DOAJ |
| description | Background: Escherichia coli (E. coli) is a major cause of urinary tract infection in children and women. Several virulence factors have been detected in uropathogenic Escherichia coli strains. P, S and Afa fimbria are the most important factors which are coding by pap, sfa and afa genes, respectively. This study aimed to determine the presence and prevalence of fimbrial virulence genes in Escherichia coli isolates from patients with urinary tract infection in Rafsanjan city, Iran.
Methods: In this study, 145 urine samples were cultured for identification of Escherichia coli. The isolates were examined via standard biochemical tests. DNA extracts from the confirmed isolates were examined to determine papEF, afaBC and sfa / focDE genes using multiplex-polymerase chain reaction (PCR) method.
Findings: Among the cultured samples, 122 Escherichia coli isolates were determined. Virulence genes genotyping of isolates showed that 38.50% of the isolates were possessed at least one of the three virulence genes. The papEF gene was the most prevalent (18.85%) and prevalences of sfa/focDE and afaBC were 2.45% and 0.81%, respectively. Twenty isolates (16.39%) contained both papEF and sfa/focDE genes.
Conclusion: The results of this study in comparison with other researchers showed that papEF gene is in the high frequency, and then, afaBC and sfa/focDE fimbrial genes. |
| first_indexed | 2024-03-12T18:47:40Z |
| format | Article |
| id | doaj.art-769354853c2a4489b2f6d1519a768d3f |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T18:47:40Z |
| publishDate | 2015-06-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-769354853c2a4489b2f6d1519a768d3f2023-08-02T07:30:57ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2015-06-01333315465531857Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract InfectionsMahbobeh Eslami0Reza Ghanbarpour1MSc Student, Department of Microbiology, Sirjan Science and Research Branch, Islamic Azad University, Sirjan, IranProfessor, Molecular Microbiology Research Group, Shahid Bahonar University, Kerman, IranBackground: Escherichia coli (E. coli) is a major cause of urinary tract infection in children and women. Several virulence factors have been detected in uropathogenic Escherichia coli strains. P, S and Afa fimbria are the most important factors which are coding by pap, sfa and afa genes, respectively. This study aimed to determine the presence and prevalence of fimbrial virulence genes in Escherichia coli isolates from patients with urinary tract infection in Rafsanjan city, Iran. Methods: In this study, 145 urine samples were cultured for identification of Escherichia coli. The isolates were examined via standard biochemical tests. DNA extracts from the confirmed isolates were examined to determine papEF, afaBC and sfa / focDE genes using multiplex-polymerase chain reaction (PCR) method. Findings: Among the cultured samples, 122 Escherichia coli isolates were determined. Virulence genes genotyping of isolates showed that 38.50% of the isolates were possessed at least one of the three virulence genes. The papEF gene was the most prevalent (18.85%) and prevalences of sfa/focDE and afaBC were 2.45% and 0.81%, respectively. Twenty isolates (16.39%) contained both papEF and sfa/focDE genes. Conclusion: The results of this study in comparison with other researchers showed that papEF gene is in the high frequency, and then, afaBC and sfa/focDE fimbrial genes.http://jims.mui.ac.ir/index.php/jims/article/view/4843Escherichia coliFimbrial genesUrinary tract infection |
| spellingShingle | Mahbobeh Eslami Reza Ghanbarpour Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections مجله دانشکده پزشکی اصفهان Escherichia coli Fimbrial genes Urinary tract infection |
| title | Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections |
| title_full | Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections |
| title_fullStr | Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections |
| title_full_unstemmed | Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections |
| title_short | Determination of P, S and Afa Fimbria Coding Genes in Escherichia Coli Isolates from Urinary Tract Infections |
| title_sort | determination of p s and afa fimbria coding genes in escherichia coli isolates from urinary tract infections |
| topic | Escherichia coli Fimbrial genes Urinary tract infection |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/4843 |
| work_keys_str_mv | AT mahbobeheslami determinationofpsandafafimbriacodinggenesinescherichiacoliisolatesfromurinarytractinfections AT rezaghanbarpour determinationofpsandafafimbriacodinggenesinescherichiacoliisolatesfromurinarytractinfections |