Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas
Sulfur mustard (HD) poses a serious threat due to its relatively simple production process. Exposure to HD in the short-term causes an inflammatory response, while long-term exposure results in DNA and RNA damage. Respiratory tract tissue models were exposed to relatively low concentrations of HD an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/9/815 |
| _version_ | 1797484872732770304 |
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| author | Elizabeth Dhummakupt Conor Jenkins Gabrielle Rizzo Allison Melka Daniel Carmany Amber Prugh Jennifer Horsmon Julie Renner Daniel Angelini |
| author_facet | Elizabeth Dhummakupt Conor Jenkins Gabrielle Rizzo Allison Melka Daniel Carmany Amber Prugh Jennifer Horsmon Julie Renner Daniel Angelini |
| author_sort | Elizabeth Dhummakupt |
| collection | DOAJ |
| description | Sulfur mustard (HD) poses a serious threat due to its relatively simple production process. Exposure to HD in the short-term causes an inflammatory response, while long-term exposure results in DNA and RNA damage. Respiratory tract tissue models were exposed to relatively low concentrations of HD and collected at 3 and 24 h post exposure. Histology, cytokine ELISAs, and mass spectrometric-based analyses were performed. Histology and ELISA data confirmed previously seen lung damage and inflammatory markers from HD exposure. The multi-omic mass spectrometry data showed variation in proteins and metabolites associated with increased inflammation, as well as DNA and RNA damage. HD exposure causes DNA and RNA damage that results in variation of proteins and metabolites that are associated with transcription, translation and cellular energy. |
| first_indexed | 2024-03-09T23:11:47Z |
| format | Article |
| id | doaj.art-7699041b7ebd4abea1db874c3f5f0ca3 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T23:11:47Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7699041b7ebd4abea1db874c3f5f0ca32023-11-23T17:44:05ZengMDPI AGMetabolites2218-19892022-08-0112981510.3390/metabo12090815Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard GasElizabeth Dhummakupt0Conor Jenkins1Gabrielle Rizzo2Allison Melka3Daniel Carmany4Amber Prugh5Jennifer Horsmon6Julie Renner7Daniel Angelini8US Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, BioSciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, BioSciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, BioSciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAExcet, Inc., Springfield, VA 22150, USAExcet, Inc., Springfield, VA 22150, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, BioSciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, Threat Agent Sciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, Threat Agent Sciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USAUS Army, Combat Capabilities Development Command (DEVCOM) Chemical Biological Center, BioSciences Division, Aberdeen Proving Ground, Edgewood, MD 21010, USASulfur mustard (HD) poses a serious threat due to its relatively simple production process. Exposure to HD in the short-term causes an inflammatory response, while long-term exposure results in DNA and RNA damage. Respiratory tract tissue models were exposed to relatively low concentrations of HD and collected at 3 and 24 h post exposure. Histology, cytokine ELISAs, and mass spectrometric-based analyses were performed. Histology and ELISA data confirmed previously seen lung damage and inflammatory markers from HD exposure. The multi-omic mass spectrometry data showed variation in proteins and metabolites associated with increased inflammation, as well as DNA and RNA damage. HD exposure causes DNA and RNA damage that results in variation of proteins and metabolites that are associated with transcription, translation and cellular energy.https://www.mdpi.com/2218-1989/12/9/815sulfur mustardlunghistologymetabolitesproteinslipids |
| spellingShingle | Elizabeth Dhummakupt Conor Jenkins Gabrielle Rizzo Allison Melka Daniel Carmany Amber Prugh Jennifer Horsmon Julie Renner Daniel Angelini Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas Metabolites sulfur mustard lung histology metabolites proteins lipids |
| title | Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas |
| title_full | Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas |
| title_fullStr | Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas |
| title_full_unstemmed | Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas |
| title_short | Proteomic, Metabolomic, and Lipidomic Analyses of Lung Tissue Exposed to Mustard Gas |
| title_sort | proteomic metabolomic and lipidomic analyses of lung tissue exposed to mustard gas |
| topic | sulfur mustard lung histology metabolites proteins lipids |
| url | https://www.mdpi.com/2218-1989/12/9/815 |
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