Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis
Background: Sepsis is defined as a systematic inflammatory reaction against infection. Despite the recent advances in medical and pediatric sciences, sepsis remains a significant life-threatening health issue. The main problem associated with this disorder is the nonspecific and non-discriminative s...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-09-01
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| Series: | Iranian Journal of Neonatology |
| Subjects: | |
| Online Access: | http://ijn.mums.ac.ir/article_9360_74135c037750abf8fdbd3a0b88a39fc2.pdf |
| _version_ | 1818904771518529536 |
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| author | Seyyede Fatemeh Shams Hassan Boskabadi Mohammad Reza Keramati Hossein Ayatollahi Sepideh Shakeri Maryam Sheikhi Mohammad Hadi Sadeghian |
| author_facet | Seyyede Fatemeh Shams Hassan Boskabadi Mohammad Reza Keramati Hossein Ayatollahi Sepideh Shakeri Maryam Sheikhi Mohammad Hadi Sadeghian |
| author_sort | Seyyede Fatemeh Shams |
| collection | DOAJ |
| description | Background: Sepsis is defined as a systematic inflammatory reaction against infection. Despite the recent advances in medical and pediatric sciences, sepsis remains a significant life-threatening health issue. The main problem associated with this disorder is the nonspecific and non-discriminative symptoms of noninfectious diseases. The present study aimed to introduce diagnostic parameters of high sensitivity and specificity for neonatal sepsis.Methods: This case-control study was conducted in Mashhad, Iran during 2013-2015. Subjects included 40 septic neonates with confirmed diagnosis by a subspecialist of pediatrics and 40 neonates without clinical and laboratory findings of sepsis as the control group. Blood samples were collected from all the infants. In addition, differential white blood cell count of peripheral blood smear was performed, and immature/total neutrophil ratio (I/T) was calculated for all the PBS slides. Plasma calprotectin levels were also determined using the sandwich ELISA method.Results: Mean plasma concentration of calprotectin was 33190±23760 and 18980±13410 ng/ml in the septic and control groups, respectively. Moreover, mean I/T was 0.61±0.22 and 0.51±0.26 in the septic and control groups, respectively. The obtained results indicated that calprotectin levels and I/T were significantly higher in the septic group compared to the control group (P |
| first_indexed | 2024-12-19T21:12:44Z |
| format | Article |
| id | doaj.art-769f2a4b56804115b2fbb729ec83c681 |
| institution | Directory Open Access Journal |
| issn | 2251-7510 2322-2158 |
| language | English |
| last_indexed | 2024-12-19T21:12:44Z |
| publishDate | 2017-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Neonatology |
| spelling | doaj.art-769f2a4b56804115b2fbb729ec83c6812022-12-21T20:05:27ZengMashhad University of Medical SciencesIranian Journal of Neonatology2251-75102322-21582017-09-0183192410.22038/ijn.2017.93609360Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal SepsisSeyyede Fatemeh Shams0Hassan Boskabadi1Mohammad Reza Keramati2Hossein Ayatollahi3Sepideh Shakeri4Maryam Sheikhi5Mohammad Hadi Sadeghian6Cancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pediatrics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranCancer Molecular Pathology Research Center, Mashhad University of Medical Sciences, Mashhad, IranBackground: Sepsis is defined as a systematic inflammatory reaction against infection. Despite the recent advances in medical and pediatric sciences, sepsis remains a significant life-threatening health issue. The main problem associated with this disorder is the nonspecific and non-discriminative symptoms of noninfectious diseases. The present study aimed to introduce diagnostic parameters of high sensitivity and specificity for neonatal sepsis.Methods: This case-control study was conducted in Mashhad, Iran during 2013-2015. Subjects included 40 septic neonates with confirmed diagnosis by a subspecialist of pediatrics and 40 neonates without clinical and laboratory findings of sepsis as the control group. Blood samples were collected from all the infants. In addition, differential white blood cell count of peripheral blood smear was performed, and immature/total neutrophil ratio (I/T) was calculated for all the PBS slides. Plasma calprotectin levels were also determined using the sandwich ELISA method.Results: Mean plasma concentration of calprotectin was 33190±23760 and 18980±13410 ng/ml in the septic and control groups, respectively. Moreover, mean I/T was 0.61±0.22 and 0.51±0.26 in the septic and control groups, respectively. The obtained results indicated that calprotectin levels and I/T were significantly higher in the septic group compared to the control group (Phttp://ijn.mums.ac.ir/article_9360_74135c037750abf8fdbd3a0b88a39fc2.pdfCalprotectinELISAImmature neutrophilsNeonatal sepsisNRBC |
| spellingShingle | Seyyede Fatemeh Shams Hassan Boskabadi Mohammad Reza Keramati Hossein Ayatollahi Sepideh Shakeri Maryam Sheikhi Mohammad Hadi Sadeghian Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis Iranian Journal of Neonatology Calprotectin ELISA Immature neutrophils Neonatal sepsis NRBC |
| title | Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis |
| title_full | Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis |
| title_fullStr | Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis |
| title_full_unstemmed | Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis |
| title_short | Evaluation of Immature Neutrophil Ratio and Calprotectin Level for the Diagnosis of Neonatal Sepsis |
| title_sort | evaluation of immature neutrophil ratio and calprotectin level for the diagnosis of neonatal sepsis |
| topic | Calprotectin ELISA Immature neutrophils Neonatal sepsis NRBC |
| url | http://ijn.mums.ac.ir/article_9360_74135c037750abf8fdbd3a0b88a39fc2.pdf |
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