The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia
Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/8/1661 |
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| author | Andrés Ramírez-López María Teresa Álvarez Román Elena Monzón Manzano Paula Acuña Elena G. Arias-Salgado Mónica Martín Salces María Isabel Rivas Pollmar Víctor Jiménez Yuste Raul Justo Sanz Sara García Barcenilla Tamara Cebanu Elena González Zorrilla Nora V. Butta |
| author_facet | Andrés Ramírez-López María Teresa Álvarez Román Elena Monzón Manzano Paula Acuña Elena G. Arias-Salgado Mónica Martín Salces María Isabel Rivas Pollmar Víctor Jiménez Yuste Raul Justo Sanz Sara García Barcenilla Tamara Cebanu Elena González Zorrilla Nora V. Butta |
| author_sort | Andrés Ramírez-López |
| collection | DOAJ |
| description | Loss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet counts, function, and apoptosis. This observational, prospective, and transversal study included 82 patients with chronic primary ITP and 115 healthy controls. We measured platelet activation markers and assayed platelet glycosylation and caspase activity, analysing samples using flow cytometry. Platelets from patients with ITP with a platelet count <30 × 10<sup>3</sup>/µL presented less sialic acid. Levels of α1,6-fucose (a glycan residue that can directly regulate antibody-dependent cellular cytotoxicity) and α-mannose (which can be recognised by mannose-binding-lectin and activate the complement pathway) were increased in the platelets from these patients. Platelet surface exposure of other glycoside residues due to sialic acid loss inversely correlated with platelet count and the ability to be activated. Moreover, loss of sialic acid induced the ingestion of platelets by human hepatome HepG2 cells. Changes in glycoside composition of glycoproteins on the platelets’ surface impaired their functional capacity and increased their apoptosis. These changes in platelet glycoside residues appeared to be related to ITP severity. |
| first_indexed | 2024-03-10T12:22:14Z |
| format | Article |
| id | doaj.art-76a13461939046d790aac313aa01bc9e |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T12:22:14Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-76a13461939046d790aac313aa01bc9e2023-11-21T15:22:50ZengMDPI AGJournal of Clinical Medicine2077-03832021-04-01108166110.3390/jcm10081661The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune ThrombocytopaeniaAndrés Ramírez-López0María Teresa Álvarez Román1Elena Monzón Manzano2Paula Acuña3Elena G. Arias-Salgado4Mónica Martín Salces5María Isabel Rivas Pollmar6Víctor Jiménez Yuste7Raul Justo Sanz8Sara García Barcenilla9Tamara Cebanu10Elena González Zorrilla11Nora V. Butta12Hematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainHematology Unit, La Paz University Hospital-IdiPAZ, Paseo de la Castellana 261, 28046 Madrid, SpainLoss of sialic acid from the carbohydrate side chains of platelet glycoproteins can affect platelet clearance, a proposed mechanism involved in the etiopathogenesis of immune thrombocytopaenia (ITP). We aimed to assess whether changes in platelet glycosylation in patients with ITP affected platelet counts, function, and apoptosis. This observational, prospective, and transversal study included 82 patients with chronic primary ITP and 115 healthy controls. We measured platelet activation markers and assayed platelet glycosylation and caspase activity, analysing samples using flow cytometry. Platelets from patients with ITP with a platelet count <30 × 10<sup>3</sup>/µL presented less sialic acid. Levels of α1,6-fucose (a glycan residue that can directly regulate antibody-dependent cellular cytotoxicity) and α-mannose (which can be recognised by mannose-binding-lectin and activate the complement pathway) were increased in the platelets from these patients. Platelet surface exposure of other glycoside residues due to sialic acid loss inversely correlated with platelet count and the ability to be activated. Moreover, loss of sialic acid induced the ingestion of platelets by human hepatome HepG2 cells. Changes in glycoside composition of glycoproteins on the platelets’ surface impaired their functional capacity and increased their apoptosis. These changes in platelet glycoside residues appeared to be related to ITP severity.https://www.mdpi.com/2077-0383/10/8/1661immune thrombocytopaeniaplatelet apoptosissialic acidplatelet activation markersglycoside residues |
| spellingShingle | Andrés Ramírez-López María Teresa Álvarez Román Elena Monzón Manzano Paula Acuña Elena G. Arias-Salgado Mónica Martín Salces María Isabel Rivas Pollmar Víctor Jiménez Yuste Raul Justo Sanz Sara García Barcenilla Tamara Cebanu Elena González Zorrilla Nora V. Butta The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia Journal of Clinical Medicine immune thrombocytopaenia platelet apoptosis sialic acid platelet activation markers glycoside residues |
| title | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
| title_full | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
| title_fullStr | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
| title_full_unstemmed | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
| title_short | The Importance of Platelet Glycoside Residues in the Haemostasis of Patients with Immune Thrombocytopaenia |
| title_sort | importance of platelet glycoside residues in the haemostasis of patients with immune thrombocytopaenia |
| topic | immune thrombocytopaenia platelet apoptosis sialic acid platelet activation markers glycoside residues |
| url | https://www.mdpi.com/2077-0383/10/8/1661 |
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