Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease
Serotonin or 5-hydroxytryptamine (5-HT) is primarily involved in the regulation of learning and memory. Pathological changes in metabolism or functional imbalance of 5-HT has been associated with Alzheimer's disease (AD). The hypothesis tested is that in peripheral blood, markers of the seroton...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844020315590 |
| _version_ | 1818538690847178752 |
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| author | Masoud Neshan Arezoo Campbell Seyed Kazem Malakouti Mahsa Zareii Ghasem Ahangari |
| author_facet | Masoud Neshan Arezoo Campbell Seyed Kazem Malakouti Mahsa Zareii Ghasem Ahangari |
| author_sort | Masoud Neshan |
| collection | DOAJ |
| description | Serotonin or 5-hydroxytryptamine (5-HT) is primarily involved in the regulation of learning and memory. Pathological changes in metabolism or functional imbalance of 5-HT has been associated with Alzheimer's disease (AD). The hypothesis tested is that in peripheral blood, markers of the serotonergic pathway can be used as a diagnostic tool for AD. The current study measured the relative expression of 5-HT receptors (5-HTR2A and 5-HTR3A) as well as the 5-HT catalytic enzyme, Monoamine oxidase A (MAO-A) mRNA in Peripheral Blood Mononuclear Cells (PBMCs) of patients with late-onset Alzheimer's disease (LOAD) and age-matched controls. 5-HTR2A, 5-HTR3A, and MAO-A mRNA expressions were examined in PBMCs of 30 patients with LOAD and 30 control individuals. Real-time quantitative PCR was used to measure mRNA expression. The dementia status of patients in this study was assessed using a Mini-Mental State Examination (MMSE). Mean data of relative mRNA expression of 5-HTR2A, 5-HTR3A and MAO-A were significantly lower in PBMCs of patients with LOAD compared with controls. Based on the down-regulation of serotonergic markers in PBMCs, our findings may be another claim to the systemic nature of LOAD. The role of peripheral serotonergic downregulation, in the pathogenesis of AD, needs to be further studied. Given the extremely convenient access to PBMCs, these molecular events may represent more complete dimensions of AD neuropathophysiology or possibly lead to a new direction in studies focused on blood-based markers. |
| first_indexed | 2024-12-11T21:32:13Z |
| format | Article |
| id | doaj.art-76a136414a554149af828f0b81a52408 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-11T21:32:13Z |
| publishDate | 2020-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-76a136414a554149af828f0b81a524082022-12-22T00:50:08ZengElsevierHeliyon2405-84402020-08-0168e04716Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's diseaseMasoud Neshan0Arezoo Campbell1Seyed Kazem Malakouti2Mahsa Zareii3Ghasem Ahangari4Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, IranDepartment of Pharmaceutical Sciences, Western University of Health Sciences, California, USAMental Health Research Center, Tehran Institute of Psychiatry–School of Behavioral Sciences and Mental Health, Iran University of Medical Sciences, Tehran, IranMental Health Research Center, Tehran Institute of Psychiatry–School of Behavioral Sciences and Mental Health, Iran University of Medical Sciences, Tehran, IranDepartment of Medical Genetics, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran; Corresponding author.Serotonin or 5-hydroxytryptamine (5-HT) is primarily involved in the regulation of learning and memory. Pathological changes in metabolism or functional imbalance of 5-HT has been associated with Alzheimer's disease (AD). The hypothesis tested is that in peripheral blood, markers of the serotonergic pathway can be used as a diagnostic tool for AD. The current study measured the relative expression of 5-HT receptors (5-HTR2A and 5-HTR3A) as well as the 5-HT catalytic enzyme, Monoamine oxidase A (MAO-A) mRNA in Peripheral Blood Mononuclear Cells (PBMCs) of patients with late-onset Alzheimer's disease (LOAD) and age-matched controls. 5-HTR2A, 5-HTR3A, and MAO-A mRNA expressions were examined in PBMCs of 30 patients with LOAD and 30 control individuals. Real-time quantitative PCR was used to measure mRNA expression. The dementia status of patients in this study was assessed using a Mini-Mental State Examination (MMSE). Mean data of relative mRNA expression of 5-HTR2A, 5-HTR3A and MAO-A were significantly lower in PBMCs of patients with LOAD compared with controls. Based on the down-regulation of serotonergic markers in PBMCs, our findings may be another claim to the systemic nature of LOAD. The role of peripheral serotonergic downregulation, in the pathogenesis of AD, needs to be further studied. Given the extremely convenient access to PBMCs, these molecular events may represent more complete dimensions of AD neuropathophysiology or possibly lead to a new direction in studies focused on blood-based markers.http://www.sciencedirect.com/science/article/pii/S2405844020315590Alzheimer's disease5-HT2A receptor5-HT3A receptorMonoamine oxidase-A (MAO-A)Gene expressionLOAD |
| spellingShingle | Masoud Neshan Arezoo Campbell Seyed Kazem Malakouti Mahsa Zareii Ghasem Ahangari Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease Heliyon Alzheimer's disease 5-HT2A receptor 5-HT3A receptor Monoamine oxidase-A (MAO-A) Gene expression LOAD |
| title | Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease |
| title_full | Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease |
| title_fullStr | Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease |
| title_full_unstemmed | Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease |
| title_short | Gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late-onset Alzheimer's disease |
| title_sort | gene expression of serotonergic markers in peripheral blood mononuclear cells of patients with late onset alzheimer s disease |
| topic | Alzheimer's disease 5-HT2A receptor 5-HT3A receptor Monoamine oxidase-A (MAO-A) Gene expression LOAD |
| url | http://www.sciencedirect.com/science/article/pii/S2405844020315590 |
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