Alteration of the oral and gut microbiota in patients with Kawasaki disease
Background Kawasaki disease (KD) is a multi-systemic vasculitis that primarily affects children and has an unknown cause. Although an increasing number of studies linking the gut microbiota with KD, the unchallengeable etiology of KD is not available. Methods Here, we obtained fecal and oral samples...
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PeerJ Inc.
2023-07-01
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| Online Access: | https://peerj.com/articles/15662.pdf |
| _version_ | 1827599432464465920 |
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| author | Qinghuang Zeng Renhe Zeng Jianbin Ye |
| author_facet | Qinghuang Zeng Renhe Zeng Jianbin Ye |
| author_sort | Qinghuang Zeng |
| collection | DOAJ |
| description | Background Kawasaki disease (KD) is a multi-systemic vasculitis that primarily affects children and has an unknown cause. Although an increasing number of studies linking the gut microbiota with KD, the unchallengeable etiology of KD is not available. Methods Here, we obtained fecal and oral samples from KD patients and healthy controls, and then we use high-throughput sequencing to examine the diversity and composition of microbiota. Results Results showed that both in the gut and oral microbiota, the diversity of KD patients was significantly lower than that of the healthy controls. In the gut microbiota, a higher abundance of Enterococcus (40.12% vs less than 0.1%), Bifidobacterium (20.71% vs 3.06%), Escherichia-Shigella (17.56% vs 0.61%), Streptococcus (5.97% vs 0.11%) and Blautia (4.69% vs 0.1%) was observed in the KD patients, and enrichment of Enterococcus in the patients was observed. In terms of oral microbiota, the prevalence of Streptococcus (21.99% vs 0.1%), Rothia (3.02% vs 0.1%), and Escherichia-Shigella (0.68% vs 0.0%) were significantly higher in the KD patients, with the enrichment of Streptococcus and Escherichia-Shigella. Additionally, significant differences in microbial community function between KD patients and healthy controls in the fecal samples were also observed, which will affect the colonization and reproduction of gut microbiota. Conclusions These results suggested that the dysbiosis of gut and oral microbiota are both related to KD pathogenesis, of which, the prevalence of Enterococcus in the gut and higher abundance of Streptococcus and Escherichia-Shigella in the oral cavity will be a potential biomarker of the KD. Overall, this study not only confirms that the disturbance of gut microbiota is a causative trigger of KD but also provides new insight into the oral microbiota involved in KD pathogenesis. |
| first_indexed | 2024-03-09T04:14:04Z |
| format | Article |
| id | doaj.art-76a21e3dc4774b30a40f27de657c6532 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T04:14:04Z |
| publishDate | 2023-07-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-76a21e3dc4774b30a40f27de657c65322023-12-03T13:57:16ZengPeerJ Inc.PeerJ2167-83592023-07-0111e1566210.7717/peerj.15662Alteration of the oral and gut microbiota in patients with Kawasaki diseaseQinghuang Zeng0Renhe Zeng1Jianbin Ye2School of Clinical Medicine, Fujian Medical University, Fuzhou, ChinaSchool of Clinical Medicine, Fujian Medical University, Fuzhou, ChinaSchool of Basic Medicine Science, Putian University, Putian, ChinaBackground Kawasaki disease (KD) is a multi-systemic vasculitis that primarily affects children and has an unknown cause. Although an increasing number of studies linking the gut microbiota with KD, the unchallengeable etiology of KD is not available. Methods Here, we obtained fecal and oral samples from KD patients and healthy controls, and then we use high-throughput sequencing to examine the diversity and composition of microbiota. Results Results showed that both in the gut and oral microbiota, the diversity of KD patients was significantly lower than that of the healthy controls. In the gut microbiota, a higher abundance of Enterococcus (40.12% vs less than 0.1%), Bifidobacterium (20.71% vs 3.06%), Escherichia-Shigella (17.56% vs 0.61%), Streptococcus (5.97% vs 0.11%) and Blautia (4.69% vs 0.1%) was observed in the KD patients, and enrichment of Enterococcus in the patients was observed. In terms of oral microbiota, the prevalence of Streptococcus (21.99% vs 0.1%), Rothia (3.02% vs 0.1%), and Escherichia-Shigella (0.68% vs 0.0%) were significantly higher in the KD patients, with the enrichment of Streptococcus and Escherichia-Shigella. Additionally, significant differences in microbial community function between KD patients and healthy controls in the fecal samples were also observed, which will affect the colonization and reproduction of gut microbiota. Conclusions These results suggested that the dysbiosis of gut and oral microbiota are both related to KD pathogenesis, of which, the prevalence of Enterococcus in the gut and higher abundance of Streptococcus and Escherichia-Shigella in the oral cavity will be a potential biomarker of the KD. Overall, this study not only confirms that the disturbance of gut microbiota is a causative trigger of KD but also provides new insight into the oral microbiota involved in KD pathogenesis.https://peerj.com/articles/15662.pdfKawasaki diseaseOral microbiotaGut microbiotaStreptococcusIllumina sequencing |
| spellingShingle | Qinghuang Zeng Renhe Zeng Jianbin Ye Alteration of the oral and gut microbiota in patients with Kawasaki disease PeerJ Kawasaki disease Oral microbiota Gut microbiota Streptococcus Illumina sequencing |
| title | Alteration of the oral and gut microbiota in patients with Kawasaki disease |
| title_full | Alteration of the oral and gut microbiota in patients with Kawasaki disease |
| title_fullStr | Alteration of the oral and gut microbiota in patients with Kawasaki disease |
| title_full_unstemmed | Alteration of the oral and gut microbiota in patients with Kawasaki disease |
| title_short | Alteration of the oral and gut microbiota in patients with Kawasaki disease |
| title_sort | alteration of the oral and gut microbiota in patients with kawasaki disease |
| topic | Kawasaki disease Oral microbiota Gut microbiota Streptococcus Illumina sequencing |
| url | https://peerj.com/articles/15662.pdf |
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