Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice
The goal of the study was to develop a novel, rapid Calibrated Short TR Recovery (CaSTRR) method to measure the brain-blood partition coefficient (BBPC) in mice. The BBPC is necessary for quantifying cerebral blood flow (CBF) using tracer-based techniques like arterial spin labeling (ASL), but previ...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fnins.2019.00308/full |
| _version_ | 1819230293291171840 |
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| author | Scott W. Thalman Scott W. Thalman David K. Powell David K. Powell Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin |
| author_facet | Scott W. Thalman Scott W. Thalman David K. Powell David K. Powell Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin |
| author_sort | Scott W. Thalman |
| collection | DOAJ |
| description | The goal of the study was to develop a novel, rapid Calibrated Short TR Recovery (CaSTRR) method to measure the brain-blood partition coefficient (BBPC) in mice. The BBPC is necessary for quantifying cerebral blood flow (CBF) using tracer-based techniques like arterial spin labeling (ASL), but previous techniques required prohibitively long acquisition times so a constant BBPC equal to 0.9 mL/g is typically used regardless of studied species, condition, or disease. An accelerated method of BBPC correction could improve regional specificity in CBF maps particularly in white matter. Male C57Bl/6N mice (n = 8) were scanned at 7T using CaSTRR to measure BBPC determine regional variability. This technique employs phase-spoiled gradient echo acquisitions with varying repetition times (TRs) to estimate proton density in the brain and a blood sample. Proton density weighted images are then calibrated to a series of phantoms with known concentrations of deuterium to determine BBPC. Pseudo-continuous ASL was also acquired to quantify CBF with and without empirical BBPC correction. Using the CaSTRR technique we demonstrate that, in mice, white matter has a significantly lower BBPC (BBPCwhite = 0.93 ± 0.05 mL/g) than cortical gray matter (BBPCgray = 0.99 ± 0.04 mL/g, p = 0.03), and that when voxel-wise BBPC correction is performed on CBF maps the observed difference in perfusion between gray and white matter is improved by as much as 14%. Our results suggest that BBPC correction is feasible and could be particularly important in future studies of perfusion in white matter pathologies. |
| first_indexed | 2024-12-23T11:26:46Z |
| format | Article |
| id | doaj.art-76a23b3c9a154542990d581169a0d386 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-23T11:26:46Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-76a23b3c9a154542990d581169a0d3862022-12-21T17:48:55ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2019-04-011310.3389/fnins.2019.00308430946Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in MiceScott W. Thalman0Scott W. Thalman1David K. Powell2David K. Powell3Ai-Ling Lin4Ai-Ling Lin5Ai-Ling Lin6Ai-Ling Lin7F. Joseph Halcomb III, MD Department of Biomedical Engineering, University of Kentucky, Lexington, KY, United StatesSanders-Brown Center on Aging, University of Kentucky, Lexington, KY, United StatesF. Joseph Halcomb III, MD Department of Biomedical Engineering, University of Kentucky, Lexington, KY, United StatesMagnetic Resonance Imaging and Spectroscopy Center, University of Kentucky, Lexington, KY, United StatesF. Joseph Halcomb III, MD Department of Biomedical Engineering, University of Kentucky, Lexington, KY, United StatesMagnetic Resonance Imaging and Spectroscopy Center, University of Kentucky, Lexington, KY, United StatesDepartment of Pharmacology and Nutritional Sciences, University of Kentucky, Lexington, KY, United StatesDepartment of Neuroscience, University of Kentucky, Lexington, KY, United StatesThe goal of the study was to develop a novel, rapid Calibrated Short TR Recovery (CaSTRR) method to measure the brain-blood partition coefficient (BBPC) in mice. The BBPC is necessary for quantifying cerebral blood flow (CBF) using tracer-based techniques like arterial spin labeling (ASL), but previous techniques required prohibitively long acquisition times so a constant BBPC equal to 0.9 mL/g is typically used regardless of studied species, condition, or disease. An accelerated method of BBPC correction could improve regional specificity in CBF maps particularly in white matter. Male C57Bl/6N mice (n = 8) were scanned at 7T using CaSTRR to measure BBPC determine regional variability. This technique employs phase-spoiled gradient echo acquisitions with varying repetition times (TRs) to estimate proton density in the brain and a blood sample. Proton density weighted images are then calibrated to a series of phantoms with known concentrations of deuterium to determine BBPC. Pseudo-continuous ASL was also acquired to quantify CBF with and without empirical BBPC correction. Using the CaSTRR technique we demonstrate that, in mice, white matter has a significantly lower BBPC (BBPCwhite = 0.93 ± 0.05 mL/g) than cortical gray matter (BBPCgray = 0.99 ± 0.04 mL/g, p = 0.03), and that when voxel-wise BBPC correction is performed on CBF maps the observed difference in perfusion between gray and white matter is improved by as much as 14%. Our results suggest that BBPC correction is feasible and could be particularly important in future studies of perfusion in white matter pathologies.https://www.frontiersin.org/article/10.3389/fnins.2019.00308/fullarterial spin labelingbrain-blood partition coefficientcerebral blood flowgray-white matter contrastmagnetic resonance imaging |
| spellingShingle | Scott W. Thalman Scott W. Thalman David K. Powell David K. Powell Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin Ai-Ling Lin Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice Frontiers in Neuroscience arterial spin labeling brain-blood partition coefficient cerebral blood flow gray-white matter contrast magnetic resonance imaging |
| title | Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice |
| title_full | Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice |
| title_fullStr | Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice |
| title_full_unstemmed | Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice |
| title_short | Novel Calibrated Short TR Recovery (CaSTRR) Method for Brain-Blood Partition Coefficient Correction Enhances Gray-White Matter Contrast in Blood Flow Measurements in Mice |
| title_sort | novel calibrated short tr recovery castrr method for brain blood partition coefficient correction enhances gray white matter contrast in blood flow measurements in mice |
| topic | arterial spin labeling brain-blood partition coefficient cerebral blood flow gray-white matter contrast magnetic resonance imaging |
| url | https://www.frontiersin.org/article/10.3389/fnins.2019.00308/full |
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