First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil
The genus <i>Ehrlichia</i> includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. <i>Ehrlichia minasensis</i> has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/10/3/265 |
| _version_ | 1797395235364405248 |
|---|---|
| author | Lívia S. Muraro Aneliza de O. Souza Tamyres N. S. Leite Stefhano L. Cândido Andréia L. T. Melo Hugo S. Toma Mariana B. Carvalho Valéria Dutra Luciano Nakazato Alejandro Cabezas-Cruz Daniel M. de Aguiar |
| author_facet | Lívia S. Muraro Aneliza de O. Souza Tamyres N. S. Leite Stefhano L. Cândido Andréia L. T. Melo Hugo S. Toma Mariana B. Carvalho Valéria Dutra Luciano Nakazato Alejandro Cabezas-Cruz Daniel M. de Aguiar |
| author_sort | Lívia S. Muraro |
| collection | DOAJ |
| description | The genus <i>Ehrlichia</i> includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. <i>Ehrlichia minasensis</i> has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist <i>Ehrlichia</i>. In the present study, we evaluated <i>Ehrlichial</i> infection in 148 Equidae presented to the Medical Clinic Department of a Veterinary Hospital from a midwestern region of Brazil. Blood samples and ticks collected from the animals were tested by Polymerase Chain Reaction (PCR) for the presence of <i>Ehrlichia</i> spp. A multigenic approach including Anaplasmataceae-specific (i.e., 16S rRNA, <i>groEL</i>, <i>gltA</i>) and <i>Ehrlichia</i>-specific (i.e., <i>dsb</i> and <i>trp36</i>) genes was used for accurate bacteria identification. Sera samples were also collected and evaluated for the detection of anti-<i>Ehrlichia</i> antibodies by indirect fluorescent antibody test (IFA). Possible associations between molecular and serological diagnostics and clinical and hematological manifestations were tested using chi-squared or Fisher’s exact tests. Sequence analysis of the <i>dsb</i> fragment revealed that three horses (2.03%) were exposed to <i>E. minasensis</i>. Sixty-one (41.2%) Equidae (58 equines and three mules), were seropositive for <i>Ehrlichia</i> spp., with antibody titers ranging between 40 and 2560. Seropositivity to ehrlichial antigens was statistically associated with tick infestation, rural origin, hypoalbuminemia and hyperproteinemia (<i>p</i> ≤ 0.05). The present study reports the first evidence of natural infection by <i>E. minasensis</i> in horses from Brazil. |
| first_indexed | 2024-03-09T00:32:32Z |
| format | Article |
| id | doaj.art-76a3e102cc4045668dc87fb01eb34b94 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:32:32Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-76a3e102cc4045668dc87fb01eb34b942023-12-11T18:26:47ZengMDPI AGPathogens2076-08172021-02-0110326510.3390/pathogens10030265First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from BrazilLívia S. Muraro0Aneliza de O. Souza1Tamyres N. S. Leite2Stefhano L. Cândido3Andréia L. T. Melo4Hugo S. Toma5Mariana B. Carvalho6Valéria Dutra7Luciano Nakazato8Alejandro Cabezas-Cruz9Daniel M. de Aguiar10Laboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilVeterinary Clinical Laboratory, Department of Veterinary Clinics, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, BrazilVeterinary Clinical Laboratory, Department of Veterinary Clinics, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, BrazilLaboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilVeterinary of Clinical, Veterinary Medicine College, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, BrazilVeterinary Medicine Department, Federal University of Lavras (UFLA), Campus Universitário, Mailbox 3037, Lavras 37200-000, BrazilVeterinary of Clinical, Veterinary Medicine College, University of Cuiabá (UNIC), Av. Manoel José de Arruda 3100, Cuiabá 78065-900, BrazilLaboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilLaboratory of Microbiology and Molecular Biology, Veterinary Hospital of the Faculty of Veterinary Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilAnses, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, F-94700 Maisons-Alfort, FranceLaboratory of Virology and Rickettsial Infections, Veterinary Hospital, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa 2367, Cuiabá 78090-900, BrazilThe genus <i>Ehrlichia</i> includes tick-borne bacterial pathogens affecting humans, domestic and wild mammals. <i>Ehrlichia minasensis</i> has been identified in different animal species and geographical locations, suggesting that this is a widely distributed and generalist <i>Ehrlichia</i>. In the present study, we evaluated <i>Ehrlichial</i> infection in 148 Equidae presented to the Medical Clinic Department of a Veterinary Hospital from a midwestern region of Brazil. Blood samples and ticks collected from the animals were tested by Polymerase Chain Reaction (PCR) for the presence of <i>Ehrlichia</i> spp. A multigenic approach including Anaplasmataceae-specific (i.e., 16S rRNA, <i>groEL</i>, <i>gltA</i>) and <i>Ehrlichia</i>-specific (i.e., <i>dsb</i> and <i>trp36</i>) genes was used for accurate bacteria identification. Sera samples were also collected and evaluated for the detection of anti-<i>Ehrlichia</i> antibodies by indirect fluorescent antibody test (IFA). Possible associations between molecular and serological diagnostics and clinical and hematological manifestations were tested using chi-squared or Fisher’s exact tests. Sequence analysis of the <i>dsb</i> fragment revealed that three horses (2.03%) were exposed to <i>E. minasensis</i>. Sixty-one (41.2%) Equidae (58 equines and three mules), were seropositive for <i>Ehrlichia</i> spp., with antibody titers ranging between 40 and 2560. Seropositivity to ehrlichial antigens was statistically associated with tick infestation, rural origin, hypoalbuminemia and hyperproteinemia (<i>p</i> ≤ 0.05). The present study reports the first evidence of natural infection by <i>E. minasensis</i> in horses from Brazil.https://www.mdpi.com/2076-0817/10/3/265horseehrlichiosisticksPCRIFA |
| spellingShingle | Lívia S. Muraro Aneliza de O. Souza Tamyres N. S. Leite Stefhano L. Cândido Andréia L. T. Melo Hugo S. Toma Mariana B. Carvalho Valéria Dutra Luciano Nakazato Alejandro Cabezas-Cruz Daniel M. de Aguiar First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil Pathogens horse ehrlichiosis ticks PCR IFA |
| title | First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil |
| title_full | First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil |
| title_fullStr | First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil |
| title_full_unstemmed | First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil |
| title_short | First Evidence of <i>Ehrlichia minasensis</i> Infection in Horses from Brazil |
| title_sort | first evidence of i ehrlichia minasensis i infection in horses from brazil |
| topic | horse ehrlichiosis ticks PCR IFA |
| url | https://www.mdpi.com/2076-0817/10/3/265 |
| work_keys_str_mv | AT liviasmuraro firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT anelizadeosouza firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT tamyresnsleite firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT stefhanolcandido firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT andreialtmelo firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT hugostoma firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT marianabcarvalho firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT valeriadutra firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT lucianonakazato firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT alejandrocabezascruz firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil AT danielmdeaguiar firstevidenceofiehrlichiaminasensisiinfectioninhorsesfrombrazil |