Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors
<p>Abstract</p> <p>Background</p> <p>Somatic mutations in the mitochondrial genome occur in numerous tumor types including brain tumors. These mutations are generally found in the hypervariable regions I and II of the displacement loop and unlikely alter mitochondrial f...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2005-08-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/5/102 |
| _version_ | 1818789267369885696 |
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| author | Seyfried Thomas N Kiebish Michael A |
| author_facet | Seyfried Thomas N Kiebish Michael A |
| author_sort | Seyfried Thomas N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Somatic mutations in the mitochondrial genome occur in numerous tumor types including brain tumors. These mutations are generally found in the hypervariable regions I and II of the displacement loop and unlikely alter mitochondrial function. Two hypervariable regions of mononucleotide repeats occur in the mouse mitochondrial genome, i.e., the origin of replication of the light strand (O<sub>L</sub>) and the Arg tRNA.</p> <p>Methods</p> <p>In this study we examined the entire mitochondrial genome in a series of chemically induced brain tumors in the C57BL/6J strain and spontaneous brain tumors in the VM mouse strain. The tumor mtDNA was compared to that of mtDNA in brain mitochondrial populations from the corresponding syngeneic mouse host strain.</p> <p>Results</p> <p>Direct sequencing revealed a few homoplasmic base pair insertions, deletions, and substitutions in the tumor cells mainly in regions of mononucleotide repeats. A heteroplasmic mutation in the 16srRNA gene was detected in a spontaneous metastatic VM brain tumor.</p> <p>Conclusion</p> <p>None of the mutations were considered pathogenic, indicating that mtDNA somatic mutations do not likely contribute to the initiation or progression of these diverse mouse brain tumors.</p> |
| first_indexed | 2024-12-18T14:36:51Z |
| format | Article |
| id | doaj.art-76a444e98f7b4a068e12e58e0bb67aae |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-18T14:36:51Z |
| publishDate | 2005-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-76a444e98f7b4a068e12e58e0bb67aae2022-12-21T21:04:29ZengBMCBMC Cancer1471-24072005-08-015110210.1186/1471-2407-5-102Absence of pathogenic mitochondrial DNA mutations in mouse brain tumorsSeyfried Thomas NKiebish Michael A<p>Abstract</p> <p>Background</p> <p>Somatic mutations in the mitochondrial genome occur in numerous tumor types including brain tumors. These mutations are generally found in the hypervariable regions I and II of the displacement loop and unlikely alter mitochondrial function. Two hypervariable regions of mononucleotide repeats occur in the mouse mitochondrial genome, i.e., the origin of replication of the light strand (O<sub>L</sub>) and the Arg tRNA.</p> <p>Methods</p> <p>In this study we examined the entire mitochondrial genome in a series of chemically induced brain tumors in the C57BL/6J strain and spontaneous brain tumors in the VM mouse strain. The tumor mtDNA was compared to that of mtDNA in brain mitochondrial populations from the corresponding syngeneic mouse host strain.</p> <p>Results</p> <p>Direct sequencing revealed a few homoplasmic base pair insertions, deletions, and substitutions in the tumor cells mainly in regions of mononucleotide repeats. A heteroplasmic mutation in the 16srRNA gene was detected in a spontaneous metastatic VM brain tumor.</p> <p>Conclusion</p> <p>None of the mutations were considered pathogenic, indicating that mtDNA somatic mutations do not likely contribute to the initiation or progression of these diverse mouse brain tumors.</p>http://www.biomedcentral.com/1471-2407/5/102 |
| spellingShingle | Seyfried Thomas N Kiebish Michael A Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors BMC Cancer |
| title | Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors |
| title_full | Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors |
| title_fullStr | Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors |
| title_full_unstemmed | Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors |
| title_short | Absence of pathogenic mitochondrial DNA mutations in mouse brain tumors |
| title_sort | absence of pathogenic mitochondrial dna mutations in mouse brain tumors |
| url | http://www.biomedcentral.com/1471-2407/5/102 |
| work_keys_str_mv | AT seyfriedthomasn absenceofpathogenicmitochondrialdnamutationsinmousebraintumors AT kiebishmichaela absenceofpathogenicmitochondrialdnamutationsinmousebraintumors |