Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid
Abstract Introduction The mechanism by which adamantinomatous craniopharyngioma (ACP) damages the hypothalamus is still unclear. Cyst fluid rich in lipids and inflammatory factors is a characteristic pathological manifestation of ACP and may play a very important role in hypothalamic injury caused b...
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| Format: | Article |
| Language: | English |
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BMC
2022-05-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12974-022-02470-6 |
| _version_ | 1818194500148789248 |
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| author | Yilamujiang Ainiwan Yiguang Chen Chaofu Mao Junxiang Peng Siyuan Chen Songtao Wei Songtao Qi Jun Pan |
| author_facet | Yilamujiang Ainiwan Yiguang Chen Chaofu Mao Junxiang Peng Siyuan Chen Songtao Wei Songtao Qi Jun Pan |
| author_sort | Yilamujiang Ainiwan |
| collection | DOAJ |
| description | Abstract Introduction The mechanism by which adamantinomatous craniopharyngioma (ACP) damages the hypothalamus is still unclear. Cyst fluid rich in lipids and inflammatory factors is a characteristic pathological manifestation of ACP and may play a very important role in hypothalamic injury caused by tumors. Objective The objective of this study was to construct a reliable animal model of ACP cyst fluid-induced hypothalamic injury and explore the specific mechanism of hypothalamic injury caused by cyst fluid. Methods An animal model was established by injecting human ACP cyst fluid into the bilateral hypothalamus of mice. ScRNA-seq was performed on the mice hypothalamus and on an ACP sample to obtain a complete gene expression profile for analysis. Data verification was performed through pathological means. Results ACP cystic fluid caused growth retardation and an increased obesity index in mice, affected the expression of the Npy, Fgfr2, Rnpc3, Sst, and Pcsk1n genes that regulate growth and energy metabolism in hypothalamic neurons, and enhanced the cellular interaction of Agrp–Mc3r. ACP cystic fluid significantly caused inflammatory activation of hypothalamic microglia. The cellular interaction of CD74–APP is significantly strengthened between inflammatory activated microglia and hypothalamic neurons. Beta-amyloid, a marker of neurodegenerative diseases, was deposited in the ACP tumor tissues and in the hypothalamus of mice injected with ACP cyst fluid. Conclusion In this study, a novel animal model of ACP cystic fluid-hypothalamic injury was established. For the first time, it was found that ACP cystic fluid can trigger inflammatory activation of microglia to damage the hypothalamus, which may be related to the upregulation of the CD74–APP interaction and deposition of β-amyloid, implying that there may be a similar mechanism between ACP cystic fluid damage to the hypothalamus and neurodegenerative diseases. |
| first_indexed | 2024-12-12T01:03:17Z |
| format | Article |
| id | doaj.art-76a8891ba47a46b5a54f593ee6a3e2e5 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-12T01:03:17Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-76a8891ba47a46b5a54f593ee6a3e2e52022-12-22T00:43:38ZengBMCJournal of Neuroinflammation1742-20942022-05-0119111810.1186/s12974-022-02470-6Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloidYilamujiang Ainiwan0Yiguang Chen1Chaofu Mao2Junxiang Peng3Siyuan Chen4Songtao Wei5Songtao Qi6Jun Pan7Department of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityDepartment of Neurosurgery, Nanfang Hospital, Southern Medical UniversityAbstract Introduction The mechanism by which adamantinomatous craniopharyngioma (ACP) damages the hypothalamus is still unclear. Cyst fluid rich in lipids and inflammatory factors is a characteristic pathological manifestation of ACP and may play a very important role in hypothalamic injury caused by tumors. Objective The objective of this study was to construct a reliable animal model of ACP cyst fluid-induced hypothalamic injury and explore the specific mechanism of hypothalamic injury caused by cyst fluid. Methods An animal model was established by injecting human ACP cyst fluid into the bilateral hypothalamus of mice. ScRNA-seq was performed on the mice hypothalamus and on an ACP sample to obtain a complete gene expression profile for analysis. Data verification was performed through pathological means. Results ACP cystic fluid caused growth retardation and an increased obesity index in mice, affected the expression of the Npy, Fgfr2, Rnpc3, Sst, and Pcsk1n genes that regulate growth and energy metabolism in hypothalamic neurons, and enhanced the cellular interaction of Agrp–Mc3r. ACP cystic fluid significantly caused inflammatory activation of hypothalamic microglia. The cellular interaction of CD74–APP is significantly strengthened between inflammatory activated microglia and hypothalamic neurons. Beta-amyloid, a marker of neurodegenerative diseases, was deposited in the ACP tumor tissues and in the hypothalamus of mice injected with ACP cyst fluid. Conclusion In this study, a novel animal model of ACP cystic fluid-hypothalamic injury was established. For the first time, it was found that ACP cystic fluid can trigger inflammatory activation of microglia to damage the hypothalamus, which may be related to the upregulation of the CD74–APP interaction and deposition of β-amyloid, implying that there may be a similar mechanism between ACP cystic fluid damage to the hypothalamus and neurodegenerative diseases.https://doi.org/10.1186/s12974-022-02470-6Adamantinomatous CraniopharyngiomaCyst FluidHypothalamusSingle-Cell RNA SequencingMicrogliaInflammation |
| spellingShingle | Yilamujiang Ainiwan Yiguang Chen Chaofu Mao Junxiang Peng Siyuan Chen Songtao Wei Songtao Qi Jun Pan Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid Journal of Neuroinflammation Adamantinomatous Craniopharyngioma Cyst Fluid Hypothalamus Single-Cell RNA Sequencing Microglia Inflammation |
| title | Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid |
| title_full | Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid |
| title_fullStr | Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid |
| title_full_unstemmed | Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid |
| title_short | Adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β-amyloid |
| title_sort | adamantinomatous craniopharyngioma cyst fluid can trigger inflammatory activation of microglia to damage the hypothalamic neurons by inducing the production of β amyloid |
| topic | Adamantinomatous Craniopharyngioma Cyst Fluid Hypothalamus Single-Cell RNA Sequencing Microglia Inflammation |
| url | https://doi.org/10.1186/s12974-022-02470-6 |
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