Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder
Objective: Previous studies have shown that Astragalus polysaccharides (APS) and Astragaloside IV (AS-IV) protect against inflammation-related cell damage and exhibit immune enhancement. Since urothelial injury may result in an overactive bladder (OAB), the aim of this study was to investigate the e...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-03-01
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| Series: | Taiwanese Journal of Obstetrics & Gynecology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1028455920300139 |
| _version_ | 1818435359107710976 |
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| author | Yung-Hsiang Chen Wen-Chi Chen Po-Len Liu Huey-Yi Chen |
| author_facet | Yung-Hsiang Chen Wen-Chi Chen Po-Len Liu Huey-Yi Chen |
| author_sort | Yung-Hsiang Chen |
| collection | DOAJ |
| description | Objective: Previous studies have shown that Astragalus polysaccharides (APS) and Astragaloside IV (AS-IV) protect against inflammation-related cell damage and exhibit immune enhancement. Since urothelial injury may result in an overactive bladder (OAB), the aim of this study was to investigate the efficacy of APS and AS-IV on urothelial injury in an experimental animal model. Materials and methods: The effects of APS and AS-IV on the proliferation and migration of primary human urothelial cells (HUCs) or primary human fibroblast cells (HFCs) were assessed using an in vitro wounding model and colorimetric thiazolyl blue assays. Sixty virgin female mice were randomized into five groups: group 1–saline-injected plus treatment with H2O, group 2–cyclophosphamide (CYP) plus treatment with H2O, group 3–CYP plus treatment with solifenacin succinate (SS; 10 mg/kg), group 4–CYP plus treatment with AS-IV (100 mg/kg), and group 5–CYP plus treatment with APS (100 mg/kg). Cystometry assessment was conducted and cell junction-associated protein zonula occludens-2 (ZO-2) expression was measured. Voiding interval values (time between voids) were assessed in mice under anesthesia. Lastly, immunohistochemistry analysis was used to confirm the location and level, respectively, of ZO-2 expression. Results: APS and AS-IV did not influence the cell viability but increased migration in HFCs compared with the controls. The OAB mice showed significantly lower voiding interval values. Voiding interval values were significantly higher in the CYP plus treatment with APS (100 mg/kg) and AS-IV (100 mg/kg) groups than in the CYP-induced OAB group. Additionally, the expression of ZO-2, a tight junction protein, was increased in the CYP plus treatment APS (100 mg/kg) and AS-IV (100 mg/kg) groups compared with the CYP-induced OAB group. Conclusion: These findings suggest that APS and AS-IV modulate urothelial wound healing, which ameliorates urinary frequency of mice treated with CYP. APS or AS-IV may have the potential benefit of acting as urothelial wound healing modulators. Keywords: Astragalus polysaccharides, Astragaloside IV, Overactive bladder, Urothelial injury, Cyclophosphamide, Cell junction-associated protein zonula occludens-2 |
| first_indexed | 2024-12-14T16:51:38Z |
| format | Article |
| id | doaj.art-76a8fbc26a134572b206c3eeab42029c |
| institution | Directory Open Access Journal |
| issn | 1028-4559 |
| language | English |
| last_indexed | 2024-12-14T16:51:38Z |
| publishDate | 2020-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Taiwanese Journal of Obstetrics & Gynecology |
| spelling | doaj.art-76a8fbc26a134572b206c3eeab42029c2022-12-21T22:54:03ZengElsevierTaiwanese Journal of Obstetrics & Gynecology1028-45592020-03-01592248255Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladderYung-Hsiang Chen0Wen-Chi Chen1Po-Len Liu2Huey-Yi Chen3Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Departments of Medical Research, Urology, and Obstetrics and Gynecology, China Medical University Hospital, Taichung, 40402, Taiwan; Department of Psychology, College of Medical and Health Science, Asia University, Taichung, 41354, TaiwanGraduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Departments of Medical Research, Urology, and Obstetrics and Gynecology, China Medical University Hospital, Taichung, 40402, TaiwanDepartment of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, Kaohsiung, 80708, TaiwanGraduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan; Departments of Medical Research, Urology, and Obstetrics and Gynecology, China Medical University Hospital, Taichung, 40402, Taiwan; Corresponding author. Graduate Institute of Integrated Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung, 40402, Taiwan. Fax: +886 4 22037690.Objective: Previous studies have shown that Astragalus polysaccharides (APS) and Astragaloside IV (AS-IV) protect against inflammation-related cell damage and exhibit immune enhancement. Since urothelial injury may result in an overactive bladder (OAB), the aim of this study was to investigate the efficacy of APS and AS-IV on urothelial injury in an experimental animal model. Materials and methods: The effects of APS and AS-IV on the proliferation and migration of primary human urothelial cells (HUCs) or primary human fibroblast cells (HFCs) were assessed using an in vitro wounding model and colorimetric thiazolyl blue assays. Sixty virgin female mice were randomized into five groups: group 1–saline-injected plus treatment with H2O, group 2–cyclophosphamide (CYP) plus treatment with H2O, group 3–CYP plus treatment with solifenacin succinate (SS; 10 mg/kg), group 4–CYP plus treatment with AS-IV (100 mg/kg), and group 5–CYP plus treatment with APS (100 mg/kg). Cystometry assessment was conducted and cell junction-associated protein zonula occludens-2 (ZO-2) expression was measured. Voiding interval values (time between voids) were assessed in mice under anesthesia. Lastly, immunohistochemistry analysis was used to confirm the location and level, respectively, of ZO-2 expression. Results: APS and AS-IV did not influence the cell viability but increased migration in HFCs compared with the controls. The OAB mice showed significantly lower voiding interval values. Voiding interval values were significantly higher in the CYP plus treatment with APS (100 mg/kg) and AS-IV (100 mg/kg) groups than in the CYP-induced OAB group. Additionally, the expression of ZO-2, a tight junction protein, was increased in the CYP plus treatment APS (100 mg/kg) and AS-IV (100 mg/kg) groups compared with the CYP-induced OAB group. Conclusion: These findings suggest that APS and AS-IV modulate urothelial wound healing, which ameliorates urinary frequency of mice treated with CYP. APS or AS-IV may have the potential benefit of acting as urothelial wound healing modulators. Keywords: Astragalus polysaccharides, Astragaloside IV, Overactive bladder, Urothelial injury, Cyclophosphamide, Cell junction-associated protein zonula occludens-2http://www.sciencedirect.com/science/article/pii/S1028455920300139 |
| spellingShingle | Yung-Hsiang Chen Wen-Chi Chen Po-Len Liu Huey-Yi Chen Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder Taiwanese Journal of Obstetrics & Gynecology |
| title | Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder |
| title_full | Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder |
| title_fullStr | Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder |
| title_full_unstemmed | Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder |
| title_short | Astragalus polysaccharides and astragaloside IV ameliorates cyclophosphamide-induced mouse model of overactive bladder |
| title_sort | astragalus polysaccharides and astragaloside iv ameliorates cyclophosphamide induced mouse model of overactive bladder |
| url | http://www.sciencedirect.com/science/article/pii/S1028455920300139 |
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