An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome
The gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/12/3020 |
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| author | Carles Rosés Juan Antonio Nieto Blanca Viadel Elisa Gallego Ana Romo-Hualde Sergio Streitenberger Fermín I. Milagro Anna Barceló |
| author_facet | Carles Rosés Juan Antonio Nieto Blanca Viadel Elisa Gallego Ana Romo-Hualde Sergio Streitenberger Fermín I. Milagro Anna Barceló |
| author_sort | Carles Rosés |
| collection | DOAJ |
| description | The gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The Dynamic-Colonic Gastrointestinal Digester consists of five interconnected compartments, double jacket vessels that simulate the physiological conditions of the stomach, the small intestine and the three colonic sections, which are the ascending colon, transverse colon and descending colon. Human faeces are required to reproduce the conditions and culture medium of the human colon, allowing the growth of the intestinal microbiota. After a stabilization period of 12 days, a food/biocompound can be introduced to study its modulatory effects during the next 14 days (treatment period). At the end of the stabilization and treatment period, samples taken from the colon compartments are analysed. The 16S rRNA gene analysis reveals the microbiota composition. The untargeted metabolomics analysis gives more than 10,000 features (metabolites/compounds). The present protocol allows in vitro testing of the modulatory effects of foods or biocompounds on gut microbiota composition and metabolic activity. |
| first_indexed | 2024-03-10T04:08:18Z |
| format | Article |
| id | doaj.art-76ad3e0a04cb42eb9df60f89d3a89ef8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T04:08:18Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-76ad3e0a04cb42eb9df60f89d3a89ef82023-11-23T08:17:40ZengMDPI AGFoods2304-81582021-12-011012302010.3390/foods10123020An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and MetabolomeCarles Rosés0Juan Antonio Nieto1Blanca Viadel2Elisa Gallego3Ana Romo-Hualde4Sergio Streitenberger5Fermín I. Milagro6Anna Barceló7Servei de Genòmica, Universitat Autònoma de Barcelona, Bellaterra, 08193 Cerdanyola del Vallés, SpainAinia, In Vitro Digestion Assays, Parque Tecnológico de Valencia, 46980 Paterna, SpainAinia, In Vitro Digestion Assays, Parque Tecnológico de Valencia, 46980 Paterna, SpainAinia, In Vitro Digestion Assays, Parque Tecnológico de Valencia, 46980 Paterna, SpainCentre for Nutrition Research, Department of Nutrition, Food Sciences and Physiology, University of Navarra, 31008 Pamplona, SpainAMC Innova, 30100 Murcia, SpainCentre for Nutrition Research, Department of Nutrition, Food Sciences and Physiology, University of Navarra, 31008 Pamplona, SpainServei de Genòmica, Universitat Autònoma de Barcelona, Bellaterra, 08193 Cerdanyola del Vallés, SpainThe gut microbiota plays a key role in gastrointestinal immune and metabolic functions and is influenced by dietary composition. An in vitro protocol simulating the physiological conditions of the digestive system helps to study the effects of foods/biocompounds on gut microbiome and metabolome. The Dynamic-Colonic Gastrointestinal Digester consists of five interconnected compartments, double jacket vessels that simulate the physiological conditions of the stomach, the small intestine and the three colonic sections, which are the ascending colon, transverse colon and descending colon. Human faeces are required to reproduce the conditions and culture medium of the human colon, allowing the growth of the intestinal microbiota. After a stabilization period of 12 days, a food/biocompound can be introduced to study its modulatory effects during the next 14 days (treatment period). At the end of the stabilization and treatment period, samples taken from the colon compartments are analysed. The 16S rRNA gene analysis reveals the microbiota composition. The untargeted metabolomics analysis gives more than 10,000 features (metabolites/compounds). The present protocol allows in vitro testing of the modulatory effects of foods or biocompounds on gut microbiota composition and metabolic activity.https://www.mdpi.com/2304-8158/10/12/3020microbiotadigestorfood compoundscolonic fermentationmetabolome |
| spellingShingle | Carles Rosés Juan Antonio Nieto Blanca Viadel Elisa Gallego Ana Romo-Hualde Sergio Streitenberger Fermín I. Milagro Anna Barceló An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome Foods microbiota digestor food compounds colonic fermentation metabolome |
| title | An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome |
| title_full | An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome |
| title_fullStr | An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome |
| title_full_unstemmed | An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome |
| title_short | An In Vitro Protocol to Study the Modulatory Effects of a Food or Biocompound on Human Gut Microbiome and Metabolome |
| title_sort | in vitro protocol to study the modulatory effects of a food or biocompound on human gut microbiome and metabolome |
| topic | microbiota digestor food compounds colonic fermentation metabolome |
| url | https://www.mdpi.com/2304-8158/10/12/3020 |
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