Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade
Vitex negundo has reputation in the traditional medicine in diarrhea and gut spasm. This study was carried out to provide possible pharmacological basis to its medicinal use in hyperactive gut disorders. In castor oil-induced diarrheal model, the crude extract of V. negundo, caused a dose-dependent...
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2013-07-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/15296 |
| _version_ | 1818346982548176896 |
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| author | Munasib Khan Abdul Jabbar Shah Anwar Hassan Gilani |
| author_facet | Munasib Khan Abdul Jabbar Shah Anwar Hassan Gilani |
| author_sort | Munasib Khan |
| collection | DOAJ |
| description | Vitex negundo has reputation in the traditional medicine in diarrhea and gut spasm. This study was carried out to provide possible pharmacological basis to its medicinal use in hyperactive gut disorders. In castor oil-induced diarrheal model, the crude extract of V. negundo, caused a dose-dependent protection (53-71%), similar to loperamide. In isolated rabbit jejunum preparation, V. negundo caused inhibition of spontaneous and high K+-induced contractions, with EC50 values of 0.36 (0.22-0.61) and 1.30 mg/mL (0.27-5.89; n=4-5), respectively, suggestive of spasmolytic activity, mediated possibly through calcium channel blockade (CCB). The CCB activity was further confirmed when pre-treatment of the tissue with V. negundo (0.03-0.3 mg/mL) caused a rightward shift in the Ca++ concentration-response curves (CRCs), similar to diltiazem or loperamide. These data indicate that the antidiarrheal and spasmolytic effects of the crude extract of V. negundo are mediated through the presence of CCB-like constituent(s). |
| first_indexed | 2024-12-13T17:26:55Z |
| format | Article |
| id | doaj.art-76b07a5ca2084cebbf75ac30246092e6 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-12-13T17:26:55Z |
| publishDate | 2013-07-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-76b07a5ca2084cebbf75ac30246092e62022-12-21T23:37:10ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882013-07-0183Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockadeMunasib Khan0Abdul Jabbar Shah1Anwar Hassan Gilani2Natural Product Research Unit; Department of Biological and Biomedical Sciences Aga Khan University Medical College, Karachi, and Department of Pharmacy, University of Malakand, Dir LowerNatural Product Research Unit; Department of Biological and Biomedical Sciences Aga Khan University Medical College, Karachi and Department of Pharmaceutical Sciences, COMSATS Institute of Information TechnologyNatural Product Research Unit; Department of Biological and Biomedical Sciences Aga Khan University Medical College, Karachi and Department of Pharmacy, College of Health Sciences, Mekelle University, Mekelle, EthiopiaVitex negundo has reputation in the traditional medicine in diarrhea and gut spasm. This study was carried out to provide possible pharmacological basis to its medicinal use in hyperactive gut disorders. In castor oil-induced diarrheal model, the crude extract of V. negundo, caused a dose-dependent protection (53-71%), similar to loperamide. In isolated rabbit jejunum preparation, V. negundo caused inhibition of spontaneous and high K+-induced contractions, with EC50 values of 0.36 (0.22-0.61) and 1.30 mg/mL (0.27-5.89; n=4-5), respectively, suggestive of spasmolytic activity, mediated possibly through calcium channel blockade (CCB). The CCB activity was further confirmed when pre-treatment of the tissue with V. negundo (0.03-0.3 mg/mL) caused a rightward shift in the Ca++ concentration-response curves (CRCs), similar to diltiazem or loperamide. These data indicate that the antidiarrheal and spasmolytic effects of the crude extract of V. negundo are mediated through the presence of CCB-like constituent(s).https://www.banglajol.info/index.php/BJP/article/view/15296Ca antagonistDiarrheaGut spasmLoperamideVitex negundo |
| spellingShingle | Munasib Khan Abdul Jabbar Shah Anwar Hassan Gilani Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade Bangladesh Journal of Pharmacology Ca antagonist Diarrhea Gut spasm Loperamide Vitex negundo |
| title | Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade |
| title_full | Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade |
| title_fullStr | Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade |
| title_full_unstemmed | Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade |
| title_short | Antidiarrheal and antispasmodic activities of Vitex negundo are mediated through calcium channel blockade |
| title_sort | antidiarrheal and antispasmodic activities of vitex negundo are mediated through calcium channel blockade |
| topic | Ca antagonist Diarrhea Gut spasm Loperamide Vitex negundo |
| url | https://www.banglajol.info/index.php/BJP/article/view/15296 |
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