Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments
Glioblastoma is the most common and lethal primary malignant brain tumor in adults. Glioblastoma stem cells (GSCs) promote and are responsible for glioblastoma intratumoral heterogeneity and therapy resistance, due to their two main features: self-renewal and differentiation. Lipids have important b...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/8/1051 |
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| author | Sveva Grande Alessandra Palma Anna Maria Luciani Pasqualino Anello Lucia Ricci-Vitiani Mariachiara Buccarelli Quintino Giorgio D’Alessandris Roberto Pallini Laura Guidoni Vincenza Viti Antonella Rosi |
| author_facet | Sveva Grande Alessandra Palma Anna Maria Luciani Pasqualino Anello Lucia Ricci-Vitiani Mariachiara Buccarelli Quintino Giorgio D’Alessandris Roberto Pallini Laura Guidoni Vincenza Viti Antonella Rosi |
| author_sort | Sveva Grande |
| collection | DOAJ |
| description | Glioblastoma is the most common and lethal primary malignant brain tumor in adults. Glioblastoma stem cells (GSCs) promote and are responsible for glioblastoma intratumoral heterogeneity and therapy resistance, due to their two main features: self-renewal and differentiation. Lipids have important biological and physiological functions that are critical for understanding the regulation and control of stem cell fate; lipid metabolism and related unsaturation levels play a possible role as the target of therapeutics to overcome glioblastoma radioresistance. This paper aimed at an in-depth analysis of 13 GSC mesenchymal (MES) lines, two subclones, and a stabilized glioblastoma line (T98G) by magnetic resonance spectroscopy (MRS). Particularly, 2D MRS was used to investigate lipid unsaturation behavior during growth in culture and after treatment with etomoxir and photon beams. MES lines, although belonging to the same genetic and metabolic cluster, showed metabolic heterogeneity when observed by MRS, focusing on lipid signals. Nonetheless, the observed unsaturation level stability for two representative lines after stressful treatments suggests unusual robustness of the unsaturation levels for each line, as a peculiar and intrinsic characteristic of GSCs. |
| first_indexed | 2024-03-09T04:40:06Z |
| format | Article |
| id | doaj.art-76b274385d72453bbdcac1cfbc8d5d3b |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T04:40:06Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-76b274385d72453bbdcac1cfbc8d5d3b2023-12-03T13:22:31ZengMDPI AGBiomolecules2218-273X2022-07-01128105110.3390/biom12081051Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical TreatmentsSveva Grande0Alessandra Palma1Anna Maria Luciani2Pasqualino Anello3Lucia Ricci-Vitiani4Mariachiara Buccarelli5Quintino Giorgio D’Alessandris6Roberto Pallini7Laura Guidoni8Vincenza Viti9Antonella Rosi10National Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 00161 Rome, ItalyInstitute of Neurosurgery, Fondazione IRCCS Policlinico Universitario A. Gemelli, 00168 Rome, ItalyInstitute of Neurosurgery, Fondazione IRCCS Policlinico Universitario A. Gemelli, 00168 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyNational Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, 00161 Rome, ItalyGlioblastoma is the most common and lethal primary malignant brain tumor in adults. Glioblastoma stem cells (GSCs) promote and are responsible for glioblastoma intratumoral heterogeneity and therapy resistance, due to their two main features: self-renewal and differentiation. Lipids have important biological and physiological functions that are critical for understanding the regulation and control of stem cell fate; lipid metabolism and related unsaturation levels play a possible role as the target of therapeutics to overcome glioblastoma radioresistance. This paper aimed at an in-depth analysis of 13 GSC mesenchymal (MES) lines, two subclones, and a stabilized glioblastoma line (T98G) by magnetic resonance spectroscopy (MRS). Particularly, 2D MRS was used to investigate lipid unsaturation behavior during growth in culture and after treatment with etomoxir and photon beams. MES lines, although belonging to the same genetic and metabolic cluster, showed metabolic heterogeneity when observed by MRS, focusing on lipid signals. Nonetheless, the observed unsaturation level stability for two representative lines after stressful treatments suggests unusual robustness of the unsaturation levels for each line, as a peculiar and intrinsic characteristic of GSCs.https://www.mdpi.com/2218-273X/12/8/1051mesenchymal stem cellsglioblastomalipidsMR spectroscopyunsaturation |
| spellingShingle | Sveva Grande Alessandra Palma Anna Maria Luciani Pasqualino Anello Lucia Ricci-Vitiani Mariachiara Buccarelli Quintino Giorgio D’Alessandris Roberto Pallini Laura Guidoni Vincenza Viti Antonella Rosi Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments Biomolecules mesenchymal stem cells glioblastoma lipids MR spectroscopy unsaturation |
| title | Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments |
| title_full | Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments |
| title_fullStr | Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments |
| title_full_unstemmed | Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments |
| title_short | Glioblastoma Stem-Like Cells (GSCs) with Mesenchymal Signature: Lipid Profiles of Mobile Lipids Obtained with MRS before and after Radio/Chemical Treatments |
| title_sort | glioblastoma stem like cells gscs with mesenchymal signature lipid profiles of mobile lipids obtained with mrs before and after radio chemical treatments |
| topic | mesenchymal stem cells glioblastoma lipids MR spectroscopy unsaturation |
| url | https://www.mdpi.com/2218-273X/12/8/1051 |
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