Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs
<b> </b>For growth-rate retardation in commercial growing pigs suffering from non-infectious diseases, no biomarker is available for early detection and prevention of the condition or for the diagnosis of affected animals. The point in question is that the underlying pathological pathway...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/7/3/31 |
| _version_ | 1828393803624480768 |
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| author | Ana M. Gutiérrez Juan Sotillo Sarah Schlosser Karin Hummel Ingrid Miller |
| author_facet | Ana M. Gutiérrez Juan Sotillo Sarah Schlosser Karin Hummel Ingrid Miller |
| author_sort | Ana M. Gutiérrez |
| collection | DOAJ |
| description | <b> </b>For growth-rate retardation in commercial growing pigs suffering from non-infectious diseases, no biomarker is available for early detection and prevention of the condition or for the diagnosis of affected animals. The point in question is that the underlying pathological pathway of the condition is still unknown and multiple nutritional or management issues could be the cause of the disease. Common health status markers such as acute phase proteins, adenosine deaminase activity or total antioxidant capacity did not show any alteration in the saliva of animals with growth-rate retardation, so other pathways should be affected. The present study investigates saliva samples from animals with the same commercial crossbreed, sex and age, comparing control pigs and pigs with growth-rate retardation. A proteomics approach based on two-dimensional gel electrophoresis including mass spectrometry together with validation experiments was applied for the search of proteins that could help understand disease mechanisms and be used for early disease detection. Two proteins were detected as possible markers of growth-rate retardation, specifically S100A12 and carbonic anhydrase VI. A decrease in innate immune response was confirmed in pigs with growth-rate retardation, however further studies should be necessary to understand the role of the different CA VI proteoforms observed. |
| first_indexed | 2024-12-10T07:43:23Z |
| format | Article |
| id | doaj.art-76b299f9402343e4b5110987153ebb54 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-12-10T07:43:23Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-76b299f9402343e4b5110987153ebb542022-12-22T01:57:14ZengMDPI AGProteomes2227-73822019-09-01733110.3390/proteomes7030031proteomes7030031Towards Understanding Non-Infectious Growth-Rate Retardation in Growing PigsAna M. Gutiérrez0Juan Sotillo1Sarah Schlosser2Karin Hummel3Ingrid Miller4Department of Animal Medicine and Surgery, BioVetMed research group, University of Murcia, 30100 Murcia, SpainDepartment of Animal Medicine and Surgery, BioVetMed research group, University of Murcia, 30100 Murcia, SpainVetCore Facility for Research, University of Veterinary Medicine of Vienna, A-1210 Vienna, AustriaVetCore Facility for Research, University of Veterinary Medicine of Vienna, A-1210 Vienna, AustriaInstitute of Medical Biochemistry, University of Veterinary Medicine of Vienna, A-1210 Vienna, Austria<b> </b>For growth-rate retardation in commercial growing pigs suffering from non-infectious diseases, no biomarker is available for early detection and prevention of the condition or for the diagnosis of affected animals. The point in question is that the underlying pathological pathway of the condition is still unknown and multiple nutritional or management issues could be the cause of the disease. Common health status markers such as acute phase proteins, adenosine deaminase activity or total antioxidant capacity did not show any alteration in the saliva of animals with growth-rate retardation, so other pathways should be affected. The present study investigates saliva samples from animals with the same commercial crossbreed, sex and age, comparing control pigs and pigs with growth-rate retardation. A proteomics approach based on two-dimensional gel electrophoresis including mass spectrometry together with validation experiments was applied for the search of proteins that could help understand disease mechanisms and be used for early disease detection. Two proteins were detected as possible markers of growth-rate retardation, specifically S100A12 and carbonic anhydrase VI. A decrease in innate immune response was confirmed in pigs with growth-rate retardation, however further studies should be necessary to understand the role of the different CA VI proteoforms observed.https://www.mdpi.com/2227-7382/7/3/31growth-rate retardationgel-based proteomicspigsalivabiomarker detection |
| spellingShingle | Ana M. Gutiérrez Juan Sotillo Sarah Schlosser Karin Hummel Ingrid Miller Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs Proteomes growth-rate retardation gel-based proteomics pig saliva biomarker detection |
| title | Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs |
| title_full | Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs |
| title_fullStr | Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs |
| title_full_unstemmed | Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs |
| title_short | Towards Understanding Non-Infectious Growth-Rate Retardation in Growing Pigs |
| title_sort | towards understanding non infectious growth rate retardation in growing pigs |
| topic | growth-rate retardation gel-based proteomics pig saliva biomarker detection |
| url | https://www.mdpi.com/2227-7382/7/3/31 |
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