RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway
Based on the positive correlation between bone mineral density and melatonin levels in blood, this study confirmed that melatonin supplementation prevents postmenopausal osteoporosis. We further confirmed that melatonin promotes an increase in intracellular calcium concentrations through the STIM1/O...
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| Format: | Article |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.851663/full |
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| author | Lili Cao Keda Yang Wei Yuan Siming Zhou Rui Zhao Shui Qiu |
| author_facet | Lili Cao Keda Yang Wei Yuan Siming Zhou Rui Zhao Shui Qiu |
| author_sort | Lili Cao |
| collection | DOAJ |
| description | Based on the positive correlation between bone mineral density and melatonin levels in blood, this study confirmed that melatonin supplementation prevents postmenopausal osteoporosis. We further confirmed that melatonin promotes an increase in intracellular calcium concentrations through the STIM1/ORAI1 pathway, thereby inducing the proliferation of osteoblasts.Introduction: Osteoporosis (OP) is a progressive, systemic bone disease that is one of the main causes of disability and death in elderly female patients. As an amine hormone produced by the human pineal gland, melatonin plays an important role in regulating bone metabolism. This study intends to investigate the relationship between melatonin levels in human blood and bone density and to suggest the efficacy of melatonin in treating osteoporosis by performing in vivo and in vitro experiments.Methods: We used liquid chromatography-tandem mass spectrometry to determine the serum melatonin levels in postmenopausal women with osteoporosis and young women with a normal bone mass. The bone density, BV/TV, Tb.Th, Tb.Sp and other indicators of postmenopausal osteoporosis and mice with a normal bone mass were detected by measuring bone density and micro-CT. The intracellular calcium ion concentration was detected using fluorescence microscopy and a full-wavelength multifunctional microplate reader, and the expression of SOCE-related genes and STIM1/ORAI1 proteins was detected using PCR and WB.Results: This study confirmed that bone density positively correlates with the melatonin level in human blood. In the animal model, melatonin supplementation reverses postmenopausal osteoporosis. We explored the internal mechanism of melatonin treatment of osteoporosis. Melatonin promotes an increase in intracellular calcium ion concentrations through the STIM1/ORAI1 pathway to induce osteoblast proliferation.Conclusions: This study provides an important theoretical basis for the clinical application of melatonin in patients with osteoporosis and helps to optimize the diagnosis and treatment of postmenopausal osteoporosis. |
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| format | Article |
| id | doaj.art-76b3cb8b660d4c3496154d3152b3ac46 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-24T09:34:25Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-76b3cb8b660d4c3496154d3152b3ac462024-04-15T12:48:18ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-03-011310.3389/fphar.2022.851663851663RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 PathwayLili Cao0Keda Yang1Wei Yuan2Siming Zhou3Rui Zhao4Shui Qiu5Department of Medical Oncology, First Hospital of China Medical University, Shenyang, ChinaDepartment of Orthopedics, First Hospital of China Medical University, Shenyang, ChinaDepartment of Orthopedics, First Hospital of China Medical University, Shenyang, ChinaDepartment of Orthopedics, First Hospital of China Medical University, Shenyang, ChinaMinimally Invasive Orthopedic Surgery Ward, Shenyang Orthopedic Hospital, Shenyang, ChinaDepartment of Orthopedics, First Hospital of China Medical University, Shenyang, ChinaBased on the positive correlation between bone mineral density and melatonin levels in blood, this study confirmed that melatonin supplementation prevents postmenopausal osteoporosis. We further confirmed that melatonin promotes an increase in intracellular calcium concentrations through the STIM1/ORAI1 pathway, thereby inducing the proliferation of osteoblasts.Introduction: Osteoporosis (OP) is a progressive, systemic bone disease that is one of the main causes of disability and death in elderly female patients. As an amine hormone produced by the human pineal gland, melatonin plays an important role in regulating bone metabolism. This study intends to investigate the relationship between melatonin levels in human blood and bone density and to suggest the efficacy of melatonin in treating osteoporosis by performing in vivo and in vitro experiments.Methods: We used liquid chromatography-tandem mass spectrometry to determine the serum melatonin levels in postmenopausal women with osteoporosis and young women with a normal bone mass. The bone density, BV/TV, Tb.Th, Tb.Sp and other indicators of postmenopausal osteoporosis and mice with a normal bone mass were detected by measuring bone density and micro-CT. The intracellular calcium ion concentration was detected using fluorescence microscopy and a full-wavelength multifunctional microplate reader, and the expression of SOCE-related genes and STIM1/ORAI1 proteins was detected using PCR and WB.Results: This study confirmed that bone density positively correlates with the melatonin level in human blood. In the animal model, melatonin supplementation reverses postmenopausal osteoporosis. We explored the internal mechanism of melatonin treatment of osteoporosis. Melatonin promotes an increase in intracellular calcium ion concentrations through the STIM1/ORAI1 pathway to induce osteoblast proliferation.Conclusions: This study provides an important theoretical basis for the clinical application of melatonin in patients with osteoporosis and helps to optimize the diagnosis and treatment of postmenopausal osteoporosis.https://www.frontiersin.org/articles/10.3389/fphar.2022.851663/fullosteoporosismelatoninpostmenopausal womenSTIM1Ca2+ |
| spellingShingle | Lili Cao Keda Yang Wei Yuan Siming Zhou Rui Zhao Shui Qiu RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway Frontiers in Pharmacology osteoporosis melatonin postmenopausal women STIM1 Ca2+ |
| title | RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway |
| title_full | RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway |
| title_fullStr | RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway |
| title_full_unstemmed | RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway |
| title_short | RETRACTED: Melatonin Mediates Osteoblast Proliferation Through the STIM1/ORAI1 Pathway |
| title_sort | retracted melatonin mediates osteoblast proliferation through the stim1 orai1 pathway |
| topic | osteoporosis melatonin postmenopausal women STIM1 Ca2+ |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.851663/full |
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