Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes
Visualizing neuronal activation on a brain-wide scale yet with cellular resolution is a fundamental technical challenge for neuroscience. This would enable analyzing how different neuronal circuits are disrupted in pathology and how they could be rescued by pharmacological treatments. Although this...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2020.569517/full |
| _version_ | 1828823692860194816 |
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| author | Alessandra Franceschini Irene Costantini Irene Costantini Francesco S. Pavone Francesco S. Pavone Francesco S. Pavone Ludovico Silvestri Ludovico Silvestri Ludovico Silvestri |
| author_facet | Alessandra Franceschini Irene Costantini Irene Costantini Francesco S. Pavone Francesco S. Pavone Francesco S. Pavone Ludovico Silvestri Ludovico Silvestri Ludovico Silvestri |
| author_sort | Alessandra Franceschini |
| collection | DOAJ |
| description | Visualizing neuronal activation on a brain-wide scale yet with cellular resolution is a fundamental technical challenge for neuroscience. This would enable analyzing how different neuronal circuits are disrupted in pathology and how they could be rescued by pharmacological treatments. Although this goal would have appeared visionary a decade ago, recent technological advances make it eventually feasible. Here, we review the latest developments in the fields of genetics, sample preparation, imaging, and image analysis that could be combined to afford whole-brain cell-resolution activation mapping. We show how the different biochemical and optical methods have been coupled to study neuronal circuits at different spatial and temporal scales, and with cell-type specificity. The inventory of techniques presented here could be useful to find the tools best suited for a specific experiment. We envision that in the next years, mapping of neuronal activation could become routine in many laboratories, allowing dissecting the neuronal counterpart of behavior. |
| first_indexed | 2024-12-12T13:41:21Z |
| format | Article |
| id | doaj.art-76b68ae0dcee4762ae4735aaa5b72505 |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-12T13:41:21Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-76b68ae0dcee4762ae4735aaa5b725052022-12-22T00:22:48ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2020-10-011410.3389/fnins.2020.569517569517Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early GenesAlessandra Franceschini0Irene Costantini1Irene Costantini2Francesco S. Pavone3Francesco S. Pavone4Francesco S. Pavone5Ludovico Silvestri6Ludovico Silvestri7Ludovico Silvestri8European Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, ItalyNational Institute of Optics, National Research Council (INO-CNR), Sesto Fiorentino, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, ItalyNational Institute of Optics, National Research Council (INO-CNR), Sesto Fiorentino, ItalyDepartment of Physics and Astronomy, University of Florence, Florence, ItalyEuropean Laboratory for Non-linear Spectroscopy (LENS), Sesto Fiorentino, ItalyNational Institute of Optics, National Research Council (INO-CNR), Sesto Fiorentino, ItalyDepartment of Physics and Astronomy, University of Florence, Florence, ItalyVisualizing neuronal activation on a brain-wide scale yet with cellular resolution is a fundamental technical challenge for neuroscience. This would enable analyzing how different neuronal circuits are disrupted in pathology and how they could be rescued by pharmacological treatments. Although this goal would have appeared visionary a decade ago, recent technological advances make it eventually feasible. Here, we review the latest developments in the fields of genetics, sample preparation, imaging, and image analysis that could be combined to afford whole-brain cell-resolution activation mapping. We show how the different biochemical and optical methods have been coupled to study neuronal circuits at different spatial and temporal scales, and with cell-type specificity. The inventory of techniques presented here could be useful to find the tools best suited for a specific experiment. We envision that in the next years, mapping of neuronal activation could become routine in many laboratories, allowing dissecting the neuronal counterpart of behavior.https://www.frontiersin.org/articles/10.3389/fnins.2020.569517/fullimmediate early genestissue clearinglight-sheet microscopyhigh-throughput microscopyimage analysiswhole-brain mapping |
| spellingShingle | Alessandra Franceschini Irene Costantini Irene Costantini Francesco S. Pavone Francesco S. Pavone Francesco S. Pavone Ludovico Silvestri Ludovico Silvestri Ludovico Silvestri Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes Frontiers in Neuroscience immediate early genes tissue clearing light-sheet microscopy high-throughput microscopy image analysis whole-brain mapping |
| title | Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes |
| title_full | Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes |
| title_fullStr | Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes |
| title_full_unstemmed | Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes |
| title_short | Dissecting Neuronal Activation on a Brain-Wide Scale With Immediate Early Genes |
| title_sort | dissecting neuronal activation on a brain wide scale with immediate early genes |
| topic | immediate early genes tissue clearing light-sheet microscopy high-throughput microscopy image analysis whole-brain mapping |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2020.569517/full |
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