Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation
Introduction: Conventional formalin-fixed, paraffin-embedded tissue provides superior cellular morphology and long-term storage. Problems with formalin fixation comprise delay of fixation and variations in the duration of fixation. Microwave assisted tissue fixation removes the use of noxious an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3754/31-%206233_E(C)_F(T)_PF1(VP)_PFA(H)_PF2(PP).pdf |
| _version_ | 1811267743296520192 |
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| author | Meenakshi Tripathi Rani Bansal Mamta Gupta Vinay Bharat |
| author_facet | Meenakshi Tripathi Rani Bansal Mamta Gupta Vinay Bharat |
| author_sort | Meenakshi Tripathi |
| collection | DOAJ |
| description | Introduction: Conventional formalin-fixed, paraffin-embedded
tissue provides superior cellular morphology and long-term
storage. Problems with formalin fixation comprise delay of fixation
and variations in the duration of fixation. Microwave assisted
tissue fixation removes the use of noxious and potentially toxic
formalin that decreases the turnaround time and creates a
personnel friendly workflow.
Material and Methods: The present study was conducted over a
period of two years. One hundred and forty paired tissue sections
were taken including both neoplastic and non-neoplastic tissues.
One of the paired tissues was fixed in formalin and the other
was fixed by using microwave irradiation in phosphate buffered
saline. Both were then processed by conventional method. Each
slide was examined and rated for the adequacy of fixation by
two pathologists in a blinded fashion using 7 parameters: Cellular
outline, cytoplasmic detail, nuclear detail, erythrocyte integrity,
lymphocyte appearance, overall morphology and overall
staining.
Results: Statistical analysis showed that sections obtained from
microwave fixed tissues were comparable to that of routinely fixed
tissue. The p-values of all parameters were not significant except
for the overall morphology for which p-value was significant
owing to loss of tissue in some cases.
Conclusion: Microwave irradiation substantially shortened the
time from specimen reception to diagnosis (turnaround time) and
allowed same-day tissue processing and diagnosis of specimens
without compromising the overall quality of the histologic
section. |
| first_indexed | 2024-04-12T21:09:33Z |
| format | Article |
| id | doaj.art-76b9190187754a10b17634bd47bb307d |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-12T21:09:33Z |
| publishDate | 2013-12-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-76b9190187754a10b17634bd47bb307d2022-12-22T03:16:38ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2013-12-017122768277310.7860/JCDR/2013/6233.3754Comparison of Routine Fixation of Tissues with Rapid Tissue FixationMeenakshi Tripathi0Rani Bansal1Mamta Gupta2Vinay Bharat3Post Graduate Student, Department of Pathology, Subharti University, Meerut, India.Professor & Head, Department of Pathology, Subharti University, Meerut, India.Assistant Professor, Department of Pathology, Subharti University, Meerut, India.Professor, Deptartment of Pathology, Subharti Medical College, Meerut, India.Introduction: Conventional formalin-fixed, paraffin-embedded tissue provides superior cellular morphology and long-term storage. Problems with formalin fixation comprise delay of fixation and variations in the duration of fixation. Microwave assisted tissue fixation removes the use of noxious and potentially toxic formalin that decreases the turnaround time and creates a personnel friendly workflow. Material and Methods: The present study was conducted over a period of two years. One hundred and forty paired tissue sections were taken including both neoplastic and non-neoplastic tissues. One of the paired tissues was fixed in formalin and the other was fixed by using microwave irradiation in phosphate buffered saline. Both were then processed by conventional method. Each slide was examined and rated for the adequacy of fixation by two pathologists in a blinded fashion using 7 parameters: Cellular outline, cytoplasmic detail, nuclear detail, erythrocyte integrity, lymphocyte appearance, overall morphology and overall staining. Results: Statistical analysis showed that sections obtained from microwave fixed tissues were comparable to that of routinely fixed tissue. The p-values of all parameters were not significant except for the overall morphology for which p-value was significant owing to loss of tissue in some cases. Conclusion: Microwave irradiation substantially shortened the time from specimen reception to diagnosis (turnaround time) and allowed same-day tissue processing and diagnosis of specimens without compromising the overall quality of the histologic section.https://jcdr.net/articles/PDF/3754/31-%206233_E(C)_F(T)_PF1(VP)_PFA(H)_PF2(PP).pdfmicrowavefixationturnaround time |
| spellingShingle | Meenakshi Tripathi Rani Bansal Mamta Gupta Vinay Bharat Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation Journal of Clinical and Diagnostic Research microwave fixation turnaround time |
| title | Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation |
| title_full | Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation |
| title_fullStr | Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation |
| title_full_unstemmed | Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation |
| title_short | Comparison of Routine Fixation of Tissues with Rapid Tissue Fixation |
| title_sort | comparison of routine fixation of tissues with rapid tissue fixation |
| topic | microwave fixation turnaround time |
| url | https://jcdr.net/articles/PDF/3754/31-%206233_E(C)_F(T)_PF1(VP)_PFA(H)_PF2(PP).pdf |
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