Optimizing the precision of laser speckle contrast imaging
Abstract Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over the recent years, significant advancements have been made in interpreting LSCI measurements and improving the technique’s accuracy. On the o...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45303-z |
| _version_ | 1797453279057149952 |
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| author | Alberto González Olmos Sharvari Zilpelwar Smrithi Sunil David A. Boas Dmitry D. Postnov |
| author_facet | Alberto González Olmos Sharvari Zilpelwar Smrithi Sunil David A. Boas Dmitry D. Postnov |
| author_sort | Alberto González Olmos |
| collection | DOAJ |
| description | Abstract Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over the recent years, significant advancements have been made in interpreting LSCI measurements and improving the technique’s accuracy. On the other hand, the method’s precision has yet to be studied in detail, despite being as important as accuracy for many biomedical applications. Here we combine simulation, theory and animal experiments to systematically evaluate and re-analyze the role of key factors defining LSCI precision—speckle-to-pixel size ratio, polarisation, exposure time and camera-related noise. We show that contrary to the established assumptions, smaller speckle size and shorter exposure time can improve the precision, while the camera choice is less critical and does not affect the signal-to-noise ratio significantly. |
| first_indexed | 2024-03-09T15:20:32Z |
| format | Article |
| id | doaj.art-76b923926e084f17b0f6e90891b276ef |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:20:32Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-76b923926e084f17b0f6e90891b276ef2023-11-26T12:51:14ZengNature PortfolioScientific Reports2045-23222023-10-0113111010.1038/s41598-023-45303-zOptimizing the precision of laser speckle contrast imagingAlberto González Olmos0Sharvari Zilpelwar1Smrithi Sunil2David A. Boas3Dmitry D. Postnov4Department of Clinical Medicine, Aarhus UniversityDepartment of Biomedical Engineering, Boston UniversityDepartment of Biomedical Engineering, Boston UniversityDepartment of Biomedical Engineering, Boston UniversityDepartment of Clinical Medicine, Aarhus UniversityAbstract Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over the recent years, significant advancements have been made in interpreting LSCI measurements and improving the technique’s accuracy. On the other hand, the method’s precision has yet to be studied in detail, despite being as important as accuracy for many biomedical applications. Here we combine simulation, theory and animal experiments to systematically evaluate and re-analyze the role of key factors defining LSCI precision—speckle-to-pixel size ratio, polarisation, exposure time and camera-related noise. We show that contrary to the established assumptions, smaller speckle size and shorter exposure time can improve the precision, while the camera choice is less critical and does not affect the signal-to-noise ratio significantly.https://doi.org/10.1038/s41598-023-45303-z |
| spellingShingle | Alberto González Olmos Sharvari Zilpelwar Smrithi Sunil David A. Boas Dmitry D. Postnov Optimizing the precision of laser speckle contrast imaging Scientific Reports |
| title | Optimizing the precision of laser speckle contrast imaging |
| title_full | Optimizing the precision of laser speckle contrast imaging |
| title_fullStr | Optimizing the precision of laser speckle contrast imaging |
| title_full_unstemmed | Optimizing the precision of laser speckle contrast imaging |
| title_short | Optimizing the precision of laser speckle contrast imaging |
| title_sort | optimizing the precision of laser speckle contrast imaging |
| url | https://doi.org/10.1038/s41598-023-45303-z |
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