Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study
Background. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Ministry of Healthcare of the Russian Federation. “Kuban State Medical University”
2021-08-01
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| Series: | Кубанский научный медицинский вестник |
| Subjects: | |
| Online Access: | https://ksma.elpub.ru/jour/article/view/2528 |
| _version_ | 1797295410640846848 |
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| author | I. V. Bazhutova O. А. Magsumova O. О. Frolov E. V. Timchenko P. Е. Timchenko D. А. Trunin S. S. Komlev V. A. Polkanova |
| author_facet | I. V. Bazhutova O. А. Magsumova O. О. Frolov E. V. Timchenko P. Е. Timchenko D. А. Trunin S. S. Komlev V. A. Polkanova |
| author_sort | I. V. Bazhutova |
| collection | DOAJ |
| description | Background. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualitative assessment of enamel mineral and organic composition dynamics using Raman spectroscopy.Methods. Raman spectroscopy was used as primary research method. The trial bench consisted of a Shamrocksr-303i high-resolution digital spectrometer with built-in DV420A-OE cooled optical detector (spectral range 200–1200 nm), RPB-785 optic fibre scattering probe integrated with LuxxMaster LML-785.0RB-04 laser source with wavelength 785 nm.Software spectrum processing was performed with Wolfram Mathematica 9. Spectra were denoised with a median filter (5 points), the approximating line (fifth order polynomial) of autofluorescent component was determined in 700–2200 cm-1 range using an iterative algorithm and then subtracted to receive isolated Raman spectra.Linear discriminant analysis (LDA) of data was performed with the IBM SPSS Statistics package. Results. The assay included 28 teeth, with 14 extracted for orthodontic indications and 14 — for chronic periodontitis. Spectral dental enamel dynamics has been established in periodontal disease and after the in-office bleaching procedure. The evidence obtained can be applicable in dental practice to verify patients at risk of periodontal disease by interpreting the tooth enamel spectral properties, as well as prior to in-office bleaching.Conclusion. We demonstrate high efficiency of Raman spectroscopy for qualitative assessment of the mineral and organic enamel composition dynamics in various settings. Raman spectroscopy is confirmed effective and versatile in various aetiologies. It was successfully applied to diagnose periodontitis by changes in the organic and mineral enamel composition and evaluate enamel after in-office bleaching. |
| first_indexed | 2024-03-07T21:47:26Z |
| format | Article |
| id | doaj.art-76bc88ed694941d493bcafe5dcdf3450 |
| institution | Directory Open Access Journal |
| issn | 1608-6228 2541-9544 |
| language | Russian |
| last_indexed | 2024-03-07T21:47:26Z |
| publishDate | 2021-08-01 |
| publisher | Ministry of Healthcare of the Russian Federation. “Kuban State Medical University” |
| record_format | Article |
| series | Кубанский научный медицинский вестник |
| spelling | doaj.art-76bc88ed694941d493bcafe5dcdf34502024-02-25T10:57:25ZrusMinistry of Healthcare of the Russian Federation. “Kuban State Medical University”Кубанский научный медицинский вестник1608-62282541-95442021-08-0128411813210.25207/1608-6228-2021-28-4-118-1321274Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised studyI. V. Bazhutova0O. А. Magsumova1O. О. Frolov2E. V. Timchenko3P. Е. Timchenko4D. А. Trunin5S. S. Komlev6V. A. Polkanova7Samara State Medical UniversitySamara State Medical UniversitySamara National Research University named after academician S.P. Korolev (Samara University)Samara National Research University named after academician S.P. Korolev (Samara University)Samara National Research University named after academician S.P. Korolev (Samara University)Samara State Medical UniversitySamara State Medical UniversitySamara State Medical UniversityBackground. Contemporary dental diagnosis should supplement clinical examination with instrumental diagnostic techniques. Raman spectroscopy has become widely adopted due to high spatial resolution, non-invasiveness, the lack of strict requirements and ease of sample preparation.Objectives. A qualitative assessment of enamel mineral and organic composition dynamics using Raman spectroscopy.Methods. Raman spectroscopy was used as primary research method. The trial bench consisted of a Shamrocksr-303i high-resolution digital spectrometer with built-in DV420A-OE cooled optical detector (spectral range 200–1200 nm), RPB-785 optic fibre scattering probe integrated with LuxxMaster LML-785.0RB-04 laser source with wavelength 785 nm.Software spectrum processing was performed with Wolfram Mathematica 9. Spectra were denoised with a median filter (5 points), the approximating line (fifth order polynomial) of autofluorescent component was determined in 700–2200 cm-1 range using an iterative algorithm and then subtracted to receive isolated Raman spectra.Linear discriminant analysis (LDA) of data was performed with the IBM SPSS Statistics package. Results. The assay included 28 teeth, with 14 extracted for orthodontic indications and 14 — for chronic periodontitis. Spectral dental enamel dynamics has been established in periodontal disease and after the in-office bleaching procedure. The evidence obtained can be applicable in dental practice to verify patients at risk of periodontal disease by interpreting the tooth enamel spectral properties, as well as prior to in-office bleaching.Conclusion. We demonstrate high efficiency of Raman spectroscopy for qualitative assessment of the mineral and organic enamel composition dynamics in various settings. Raman spectroscopy is confirmed effective and versatile in various aetiologies. It was successfully applied to diagnose periodontitis by changes in the organic and mineral enamel composition and evaluate enamel after in-office bleaching.https://ksma.elpub.ru/jour/article/view/2528dental enamelperiodontitisin-office bleachingorganic compositionmineral compositionraman spectroscopyoptical diagnosis |
| spellingShingle | I. V. Bazhutova O. А. Magsumova O. О. Frolov E. V. Timchenko P. Е. Timchenko D. А. Trunin S. S. Komlev V. A. Polkanova Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study Кубанский научный медицинский вестник dental enamel periodontitis in-office bleaching organic composition mineral composition raman spectroscopy optical diagnosis |
| title | Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study |
| title_full | Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study |
| title_fullStr | Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study |
| title_full_unstemmed | Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study |
| title_short | Raman spectroscopy analysis of dental enamel organic and mineral composition: an experimental non-randomised study |
| title_sort | raman spectroscopy analysis of dental enamel organic and mineral composition an experimental non randomised study |
| topic | dental enamel periodontitis in-office bleaching organic composition mineral composition raman spectroscopy optical diagnosis |
| url | https://ksma.elpub.ru/jour/article/view/2528 |
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