Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma
Genome-wide studies of transcripts expression help in systematic monitoring of genes and allow targeting of candidate genes for future research. In contrast to relatively stable genomic data, the expression of genes is dynamic and regulated both at time and space level at different level in. The var...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01602/full |
| _version_ | 1819100743471202304 |
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| author | Vivek Sharma Richa Salwan P. N. Sharma Arvind Gulati |
| author_facet | Vivek Sharma Richa Salwan P. N. Sharma Arvind Gulati |
| author_sort | Vivek Sharma |
| collection | DOAJ |
| description | Genome-wide studies of transcripts expression help in systematic monitoring of genes and allow targeting of candidate genes for future research. In contrast to relatively stable genomic data, the expression of genes is dynamic and regulated both at time and space level at different level in. The variation in the rate of translation is specific for each protein. Both the inherent nature of an mRNA molecule to be translated and the external environmental stimuli can affect the efficiency of the translation process. In biocontrol agents (BCAs), the molecular response at translational level may represents noise-like response of absolute transcript level and an adaptive response to physiological and pathological situations representing subset of mRNAs population actively translated in a cell. The molecular responses of biocontrol are complex and involve multistage regulation of number of genes. The use of high-throughput techniques has led to rapid increase in volume of transcriptomics data of Trichoderma. In general, almost half of the variations of transcriptome and protein level are due to translational control. Thus, studies are required to integrate raw information from different “omics” approaches for accurate depiction of translational response of BCAs in interaction with plants and plant pathogens. The studies on translational status of only active mRNAs bridging with proteome data will help in accurate characterization of only a subset of mRNAs actively engaged in translation. This review highlights the associated bottlenecks and use of state-of-the-art procedures in addressing the gap to accelerate future accomplishment of biocontrol mechanisms. |
| first_indexed | 2024-12-22T01:07:37Z |
| format | Article |
| id | doaj.art-76c2fe71411e4a3696296cf4da9e3c9a |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T01:07:37Z |
| publishDate | 2017-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-76c2fe71411e4a3696296cf4da9e3c9a2022-12-21T18:44:03ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-08-01810.3389/fmicb.2017.01602275453Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of TrichodermaVivek Sharma0Richa Salwan1P. N. Sharma2Arvind Gulati3Department of Plant Pathology, Choudhary Sarwan Kumar Himachal Pradesh Agricultural UniversityPalampur, IndiaDepartment of Veterinary Microbiology, Choudhary Sarwan Kumar Himachal Pradesh Agricultural UniversityPalampur, IndiaDepartment of Plant Pathology, Choudhary Sarwan Kumar Himachal Pradesh Agricultural UniversityPalampur, IndiaInstitute of Himalayan Bioresource TechnologyPalampur, IndiaGenome-wide studies of transcripts expression help in systematic monitoring of genes and allow targeting of candidate genes for future research. In contrast to relatively stable genomic data, the expression of genes is dynamic and regulated both at time and space level at different level in. The variation in the rate of translation is specific for each protein. Both the inherent nature of an mRNA molecule to be translated and the external environmental stimuli can affect the efficiency of the translation process. In biocontrol agents (BCAs), the molecular response at translational level may represents noise-like response of absolute transcript level and an adaptive response to physiological and pathological situations representing subset of mRNAs population actively translated in a cell. The molecular responses of biocontrol are complex and involve multistage regulation of number of genes. The use of high-throughput techniques has led to rapid increase in volume of transcriptomics data of Trichoderma. In general, almost half of the variations of transcriptome and protein level are due to translational control. Thus, studies are required to integrate raw information from different “omics” approaches for accurate depiction of translational response of BCAs in interaction with plants and plant pathogens. The studies on translational status of only active mRNAs bridging with proteome data will help in accurate characterization of only a subset of mRNAs actively engaged in translation. This review highlights the associated bottlenecks and use of state-of-the-art procedures in addressing the gap to accelerate future accomplishment of biocontrol mechanisms.http://journal.frontiersin.org/article/10.3389/fmicb.2017.01602/fulltranscriptsactive mRNAregulationintegrated omictranslatome |
| spellingShingle | Vivek Sharma Richa Salwan P. N. Sharma Arvind Gulati Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma Frontiers in Microbiology transcripts active mRNA regulation integrated omic translatome |
| title | Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma |
| title_full | Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma |
| title_fullStr | Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma |
| title_full_unstemmed | Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma |
| title_short | Integrated Translatome and Proteome: Approach for Accurate Portraying of Widespread Multifunctional Aspects of Trichoderma |
| title_sort | integrated translatome and proteome approach for accurate portraying of widespread multifunctional aspects of trichoderma |
| topic | transcripts active mRNA regulation integrated omic translatome |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.01602/full |
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