Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel
Nematodes belonging to the genus Contracaecum (family: Anisakidae) are heteroxenous parasites with a complex life cycle. Contracaecum larvae infecting farmed fish and fishery products are economically important causing market rejection in massive infection and may have zoonotic potential. In Israel,...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Food and Waterborne Parasitology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S240567662200004X |
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| author | Nadav Davidovich Perla Tedesco Monica Caffara Daniel Yasur-Landau Andrea Gustinelli Vladimir Drabkin Ekaterina Minkova Ortal Aflalo Danny Morick Maria Letizia Fioravanti |
| author_facet | Nadav Davidovich Perla Tedesco Monica Caffara Daniel Yasur-Landau Andrea Gustinelli Vladimir Drabkin Ekaterina Minkova Ortal Aflalo Danny Morick Maria Letizia Fioravanti |
| author_sort | Nadav Davidovich |
| collection | DOAJ |
| description | Nematodes belonging to the genus Contracaecum (family: Anisakidae) are heteroxenous parasites with a complex life cycle. Contracaecum larvae infecting farmed fish and fishery products are economically important causing market rejection in massive infection and may have zoonotic potential. In Israel, Contracaecum larvae have been described morphologically in several fish species; however, none of these descriptions were supported by molecular tools. In 2019–2020, hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus), farmed in polyculture were found to be heavily infected with nematodes referable to Contracaecum larvae. Prevalence of infection in hybrid tilapia and red drum was 53.8% and 40.9%, respectively. A combined (morphological and molecular) approach revealed that both infected fish species were parasitized by the same species of Contracaecum, although larvae in hybrid tilapia were localized in the pericardial cavity whereas in red drum, they were observed in the abdominal cavity. Genetic analysis of internal transcribed spacer rDNA and cox2 mtDNA showed high similarity to unidentified Contracaecum larvae detected in several fish species in Ethiopia, Egypt and Kenya. In this study, molecular and morphological analyses place the possible new species in the C. multipapillatum complex and was provisionally named C. multipapillatum E. Further analyses combining morphological and molecular approaches are required on adult specimens collected from piscivorous birds living in the same area to support the identification of a potentially new species. |
| first_indexed | 2024-04-12T22:59:16Z |
| format | Article |
| id | doaj.art-76c6594135a24ef98c173b848bd2785b |
| institution | Directory Open Access Journal |
| issn | 2405-6766 |
| language | English |
| last_indexed | 2024-04-12T22:59:16Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food and Waterborne Parasitology |
| spelling | doaj.art-76c6594135a24ef98c173b848bd2785b2022-12-22T03:13:07ZengElsevierFood and Waterborne Parasitology2405-67662022-03-0126e00147Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in IsraelNadav Davidovich0Perla Tedesco1Monica Caffara2Daniel Yasur-Landau3Andrea Gustinelli4Vladimir Drabkin5Ekaterina Minkova6Ortal Aflalo7Danny Morick8Maria Letizia Fioravanti9Israeli Veterinary Services, Bet Dagan 5025001, Israel; Morris Kahn Marine Research Station, University of Haifa, Haifa 3498838, Israel; Corresponding author at: Israeli Veterinary Services, Bet Dagan 5025001, Israel.Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, ItalyDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, Italy; Corresponding author at: Department of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, Italy.Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 5025001, IsraelDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, ItalyFishery Product Laboratory, Kimron Veterinary Institute, Bet Dagan 5025001, IsraelThe Agency for the Veterinary Inspection of Food, Lod 7129466, IsraelThe Agency for the Veterinary Inspection of Food, Lod 7129466, IsraelMorris Kahn Marine Research Station, University of Haifa, Haifa 3498838, Israel; Hong Kong Branch of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong, ChinaDepartment of Veterinary Medical Sciences, Alma Mater Studiorum University of Bologna, Via Tolara di Sopra 50, 40064 Ozzano Emilia, BO, ItalyNematodes belonging to the genus Contracaecum (family: Anisakidae) are heteroxenous parasites with a complex life cycle. Contracaecum larvae infecting farmed fish and fishery products are economically important causing market rejection in massive infection and may have zoonotic potential. In Israel, Contracaecum larvae have been described morphologically in several fish species; however, none of these descriptions were supported by molecular tools. In 2019–2020, hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus), farmed in polyculture were found to be heavily infected with nematodes referable to Contracaecum larvae. Prevalence of infection in hybrid tilapia and red drum was 53.8% and 40.9%, respectively. A combined (morphological and molecular) approach revealed that both infected fish species were parasitized by the same species of Contracaecum, although larvae in hybrid tilapia were localized in the pericardial cavity whereas in red drum, they were observed in the abdominal cavity. Genetic analysis of internal transcribed spacer rDNA and cox2 mtDNA showed high similarity to unidentified Contracaecum larvae detected in several fish species in Ethiopia, Egypt and Kenya. In this study, molecular and morphological analyses place the possible new species in the C. multipapillatum complex and was provisionally named C. multipapillatum E. Further analyses combining morphological and molecular approaches are required on adult specimens collected from piscivorous birds living in the same area to support the identification of a potentially new species.http://www.sciencedirect.com/science/article/pii/S240567662200004XContracaecum larvaeZoonosisHybrid tilapiaRed drumIsrael |
| spellingShingle | Nadav Davidovich Perla Tedesco Monica Caffara Daniel Yasur-Landau Andrea Gustinelli Vladimir Drabkin Ekaterina Minkova Ortal Aflalo Danny Morick Maria Letizia Fioravanti Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel Food and Waterborne Parasitology Contracaecum larvae Zoonosis Hybrid tilapia Red drum Israel |
| title | Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel |
| title_full | Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel |
| title_fullStr | Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel |
| title_full_unstemmed | Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel |
| title_short | Morphological description and molecular characterization of Contracaecum larvae (Nematoda: Anisakidae) parasitizing market-size hybrid tilapia (Oreochromis aureus x Oreochromis niloticus) and red drum (Sciaenops ocellatus) farmed in Israel |
| title_sort | morphological description and molecular characterization of contracaecum larvae nematoda anisakidae parasitizing market size hybrid tilapia oreochromis aureus x oreochromis niloticus and red drum sciaenops ocellatus farmed in israel |
| topic | Contracaecum larvae Zoonosis Hybrid tilapia Red drum Israel |
| url | http://www.sciencedirect.com/science/article/pii/S240567662200004X |
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