AVALIAÇÃO DA INTEGRIDADE DE MEMBRANA EM ESPERMATOZÓIDE BOVINO CRIOPRESERVADO PARA PREVER O ÍNDICE DE PRENHEZ
Semen of twelve Tabapuã young bulls, which were according to patterns established by CBRA (1998) after cryopreservation, was submitted to fast and slow thermoresistence tests, hypoosmotic swelling test, induced acrosome reaction by heparin, sperm viability test by Trypanblue post-freezing and post-i...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade Federal de Goiás
2011-09-01
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| Series: | Ciência Animal Brasileira |
| Subjects: | |
| Online Access: | http://www.revistas.ufg.br/index.php/vet/article/view/9739/9712 |
| Summary: | Semen of twelve Tabapuã young bulls, which were according to patterns established by CBRA (1998) after cryopreservation, was submitted to fast and slow thermoresistence tests, hypoosmotic swelling test, induced acrosome reaction by heparin, sperm viability test by Trypanblue post-freezing and post-incubation with heparin, and pregnancy rates at first artificial insemination of Nellore heifers. Continuous variable averages of the mentioned tests were compared by Student–Newman–Keuls test and subjective variables were compared by Kruskal-Wallis nonparametric test (p<0.05). Simple correlation among allvariables, and multiple regression models to predict pregnancyrate of Nellore heifers, using semen of Tabapuã bulls, wereestimated. It was shown that none of the variables studied toevaluate membrane integrity in frozen semen was isolately effective (p>0.05) to preview pregnancy rate, even if using the multiple regression model that included all variables. One can conclude that the model proposed in this study was not effective to predict pregnancy rate. Hypoosmotic Swelling Test can be suggested to evaluate functional and structural integrities of plasma membrane and the capacity of spermatozoa going through acrosome reaction. |
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| ISSN: | 1518-2797 1089-6891 |