Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice
Abstract Background: The cardiac hypertrophy (CH) model for mice has been widely used, thereby providing an effective research foundation for CH exploration. Objective: To research the effects of CH modeling under abdominal aortic constriction (AAC) using different needles and weights in mice. Me...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Cardiologia (SBC)
|
| Series: | Arquivos Brasileiros de Cardiologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018000600568&lng=en&tlng=en |
| _version_ | 1811345312830193664 |
|---|---|
| author | Zhen Jia Zhijun Chen Hongfei Xu Malik Akuffu Armah Peng Teng Weidong Li Dongdong Jian Liang Ma Yiming Ni |
| author_facet | Zhen Jia Zhijun Chen Hongfei Xu Malik Akuffu Armah Peng Teng Weidong Li Dongdong Jian Liang Ma Yiming Ni |
| author_sort | Zhen Jia |
| collection | DOAJ |
| description | Abstract Background: The cardiac hypertrophy (CH) model for mice has been widely used, thereby providing an effective research foundation for CH exploration. Objective: To research the effects of CH modeling under abdominal aortic constriction (AAC) using different needles and weights in mice. Methods: Four needles with different external diameters (0.35, 0.40, 0.45, and 0.50 mm) were used for AAC. 150 male C57BL/6 mice were selected according to body weight (BW) and divided into 3 weight levels: 18 g, 22 g, and 26 g (n = 50 in each group). All weight levels were divided into 5 groups: a sham group (n = 10) and 4 AAC groups using 4 ligation intensities (n = 10 per group). After surgery, survival rates were recorded, echocardiography was performed, hearts were dissected and used for histological detection, and data were statistically analyzed, P < 0.05 was considered statistically significant. Results: All mice died in the following AAC groups: 18g/0.35 mm, 22 g/0.35 mm, 26 g/0.35 mm, 22 g/0.40 mm, and 26 g/0.40 mm. All mice with AAC, those ligated with a 0.50-mm needle, and those that underwent sham operation survived. Different death rates occurred in the following AAC groups: 18 g/0.40 mm, 18 g/0.45 mm, 18 g/0.50 mm, 22 g/45 mm, 22 g/0.50 mm, 26 g/0.45 mm, and 26 g/0.50 mm. The heart weight/body weight ratios (5.39 ± 0.85, 6.41 ± 0.68, 4.67 ± 0.37, 5.22 ± 0.42, 4.23 ± 0.28, 5.41 ± 0.14, and 4.02 ± 0.13) were significantly increased compared with those of the sham groups for mice with the same weight levels. Conclusion: A 0.45-mm needle led to more obvious CH than did 0.40-mm and 0.50-mm needles and caused extraordinary CH in 18-g mice. |
| first_indexed | 2024-04-13T20:01:16Z |
| format | Article |
| id | doaj.art-76c828e9dd1345c1af649505368440fb |
| institution | Directory Open Access Journal |
| issn | 1678-4170 |
| language | English |
| last_indexed | 2024-04-13T20:01:16Z |
| publisher | Sociedade Brasileira de Cardiologia (SBC) |
| record_format | Article |
| series | Arquivos Brasileiros de Cardiologia |
| spelling | doaj.art-76c828e9dd1345c1af649505368440fb2022-12-22T02:32:12ZengSociedade Brasileira de Cardiologia (SBC)Arquivos Brasileiros de Cardiologia1678-4170110656857610.5935/abc.20180088S0066-782X2018000600568Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in MiceZhen JiaZhijun ChenHongfei XuMalik Akuffu ArmahPeng TengWeidong LiDongdong JianLiang MaYiming NiAbstract Background: The cardiac hypertrophy (CH) model for mice has been widely used, thereby providing an effective research foundation for CH exploration. Objective: To research the effects of CH modeling under abdominal aortic constriction (AAC) using different needles and weights in mice. Methods: Four needles with different external diameters (0.35, 0.40, 0.45, and 0.50 mm) were used for AAC. 150 male C57BL/6 mice were selected according to body weight (BW) and divided into 3 weight levels: 18 g, 22 g, and 26 g (n = 50 in each group). All weight levels were divided into 5 groups: a sham group (n = 10) and 4 AAC groups using 4 ligation intensities (n = 10 per group). After surgery, survival rates were recorded, echocardiography was performed, hearts were dissected and used for histological detection, and data were statistically analyzed, P < 0.05 was considered statistically significant. Results: All mice died in the following AAC groups: 18g/0.35 mm, 22 g/0.35 mm, 26 g/0.35 mm, 22 g/0.40 mm, and 26 g/0.40 mm. All mice with AAC, those ligated with a 0.50-mm needle, and those that underwent sham operation survived. Different death rates occurred in the following AAC groups: 18 g/0.40 mm, 18 g/0.45 mm, 18 g/0.50 mm, 22 g/45 mm, 22 g/0.50 mm, 26 g/0.45 mm, and 26 g/0.50 mm. The heart weight/body weight ratios (5.39 ± 0.85, 6.41 ± 0.68, 4.67 ± 0.37, 5.22 ± 0.42, 4.23 ± 0.28, 5.41 ± 0.14, and 4.02 ± 0.13) were significantly increased compared with those of the sham groups for mice with the same weight levels. Conclusion: A 0.45-mm needle led to more obvious CH than did 0.40-mm and 0.50-mm needles and caused extraordinary CH in 18-g mice.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018000600568&lng=en&tlng=enCardiomegalyBody WeightHeart FailureNeedles/utilizationRats |
| spellingShingle | Zhen Jia Zhijun Chen Hongfei Xu Malik Akuffu Armah Peng Teng Weidong Li Dongdong Jian Liang Ma Yiming Ni Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice Arquivos Brasileiros de Cardiologia Cardiomegaly Body Weight Heart Failure Needles/utilization Rats |
| title | Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice |
| title_full | Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice |
| title_fullStr | Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice |
| title_full_unstemmed | Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice |
| title_short | Pressure Overload-induced Cardiac Hypertrophy Varies According to Different Ligation Needle Sizes and Body Weights in Mice |
| title_sort | pressure overload induced cardiac hypertrophy varies according to different ligation needle sizes and body weights in mice |
| topic | Cardiomegaly Body Weight Heart Failure Needles/utilization Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0066-782X2018000600568&lng=en&tlng=en |
| work_keys_str_mv | AT zhenjia pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT zhijunchen pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT hongfeixu pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT malikakuffuarmah pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT pengteng pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT weidongli pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT dongdongjian pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT liangma pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice AT yimingni pressureoverloadinducedcardiachypertrophyvariesaccordingtodifferentligationneedlesizesandbodyweightsinmice |