ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles
Synthesis of the MIL-100 metal-organic framework particles was carried out by hydrothermal (HT) and microwave (MW)-assisted methods. Transmission electron microscopy showed formation of microparticles in the course of hydrothermal synthesis and nanoparticles for microwave-assisted synthesis. Powder...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/24/9758 |
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| author | Ivan E. Gorban Mikhail A. Soldatov Vera V. Butova Pavel V. Medvedev Olga A. Burachevskaya Anna Belanova Peter Zolotukhin Alexander V. Soldatov |
| author_facet | Ivan E. Gorban Mikhail A. Soldatov Vera V. Butova Pavel V. Medvedev Olga A. Burachevskaya Anna Belanova Peter Zolotukhin Alexander V. Soldatov |
| author_sort | Ivan E. Gorban |
| collection | DOAJ |
| description | Synthesis of the MIL-100 metal-organic framework particles was carried out by hydrothermal (HT) and microwave (MW)-assisted methods. Transmission electron microscopy showed formation of microparticles in the course of hydrothermal synthesis and nanoparticles for microwave-assisted synthesis. Powder X-ray diffraction confirmed formation of larger crystallites for hydrothermal synthesis. Particle aggregation in aqueous solution was observed by dynamic light scattering. However, the stability of both samples could be improved in acetic acid solution. Nitrogen sorption isotherms showed high porosity of the particles. ᶫ-leucine molecule was used as a model molecule for loading in the porous micro- and nanoparticles. Loading was estimated by FTIR spectroscopy and thermogravimetric analysis. UV-VIS spectroscopy quantified ᶫ-leucine release from the particles in aqueous solution. Cytotoxicity studies using the HeLa cell model showed that the original particles were somewhat toxic, but ᶫ-leucine loading ameliorated the toxic effects, likely due to signaling properties of the amino acid. |
| first_indexed | 2024-03-10T13:53:31Z |
| format | Article |
| id | doaj.art-76c9535b9ccd4054bb35cbbe9e9e0b4e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T13:53:31Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-76c9535b9ccd4054bb35cbbe9e9e0b4e2023-11-21T01:52:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-12-012124975810.3390/ijms21249758ᶫ-Leucine Loading and Release in MIL-100 NanoparticlesIvan E. Gorban0Mikhail A. Soldatov1Vera V. Butova2Pavel V. Medvedev3Olga A. Burachevskaya4Anna Belanova5Peter Zolotukhin6Alexander V. Soldatov7The Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaResearch Laboratory “Biomedicine”, Southern Federal University, Stachki av. 194/1, 344090 Rostov-on-Don, RussiaThe Smart Materials Research Institute, Southern Federal University, Sladkova Street 178/24, 344090 Rostov-on-Don, RussiaSynthesis of the MIL-100 metal-organic framework particles was carried out by hydrothermal (HT) and microwave (MW)-assisted methods. Transmission electron microscopy showed formation of microparticles in the course of hydrothermal synthesis and nanoparticles for microwave-assisted synthesis. Powder X-ray diffraction confirmed formation of larger crystallites for hydrothermal synthesis. Particle aggregation in aqueous solution was observed by dynamic light scattering. However, the stability of both samples could be improved in acetic acid solution. Nitrogen sorption isotherms showed high porosity of the particles. ᶫ-leucine molecule was used as a model molecule for loading in the porous micro- and nanoparticles. Loading was estimated by FTIR spectroscopy and thermogravimetric analysis. UV-VIS spectroscopy quantified ᶫ-leucine release from the particles in aqueous solution. Cytotoxicity studies using the HeLa cell model showed that the original particles were somewhat toxic, but ᶫ-leucine loading ameliorated the toxic effects, likely due to signaling properties of the amino acid.https://www.mdpi.com/1422-0067/21/24/9758MOF nanoparticlesMIL-100targeted drug deliveryleucinenanomedicine |
| spellingShingle | Ivan E. Gorban Mikhail A. Soldatov Vera V. Butova Pavel V. Medvedev Olga A. Burachevskaya Anna Belanova Peter Zolotukhin Alexander V. Soldatov ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles International Journal of Molecular Sciences MOF nanoparticles MIL-100 targeted drug delivery leucine nanomedicine |
| title | ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles |
| title_full | ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles |
| title_fullStr | ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles |
| title_full_unstemmed | ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles |
| title_short | ᶫ-Leucine Loading and Release in MIL-100 Nanoparticles |
| title_sort | ᶫ leucine loading and release in mil 100 nanoparticles |
| topic | MOF nanoparticles MIL-100 targeted drug delivery leucine nanomedicine |
| url | https://www.mdpi.com/1422-0067/21/24/9758 |
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