Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials
In the current study, we screened 46 isolates of lactic acid bacteria (LAB) derived from goat milk for bacteriocin producers that can inhibit common foodborne pathogens (<i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, and <i>Bacillus cereus</i>). The...
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MDPI AG
2023-03-01
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/849 |
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| author | Zhenzhu Yang Ling Ni Wei Tian Hai Chi |
| author_facet | Zhenzhu Yang Ling Ni Wei Tian Hai Chi |
| author_sort | Zhenzhu Yang |
| collection | DOAJ |
| description | In the current study, we screened 46 isolates of lactic acid bacteria (LAB) derived from goat milk for bacteriocin producers that can inhibit common foodborne pathogens (<i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, and <i>Bacillus cereus</i>). The following three strains that showed antimicrobial activity against all indicators were identified: <i>Enterococcus faecalis</i> DH9003 and DH9012, and <i>Lactococcus lactis</i> DH9011. Their antimicrobial products exhibited typical bacteriocin characteristics, such as heat stability and proteinase nature. The bacteriostatic activity of concentrated bacteriocins produced by these LAB was observed at low concentrations (half-minimum inhibitory concentration [MIC50] and 4MIC50), whereas complete inhibition activity against <i>Listeria monocytogenes</i> was detected at high concentrations (16MIC50) of the two <i>Enterococcus faecalis</i> strains (DH9003 and DH9012). Furthermore, the probiotic potentials of the three strains were investigated and described. The results revealed that none of the strains had hemolytic activity, whereas all: were sensitive to ampicillin (50 mg/mL) and streptomycin sulfate (100 mg/mL); were resistant to bile, artificial simulated intestinal tract, and gastric juice at different pH levels (2.5, 3.0, 3.5); and had β-galactosidase activity. Furthermore, all strains exhibited an auto-aggregating phenotype, with self-aggregation ranging from 30% to 55%. DH9003 and DH9012 co-aggregated well with <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> (52.6% and 63.2%, 68.5% and 57.6%, respectively), whereas DH9011 co-aggregated poorly with <i>Listeria monocytogenes</i> (15.6%) and did not co-aggregate with Escherichia coli. Furthermore, our results showed that all three isolates exhibited strong antibacterial activity, tolerance to bile and simulated gastrointestinal environments, adhesion capability, and safety. Finally, DH9003 was selected and used for gavage in rats. By observing the pathological characteristics of rat intestinal and liver tissue sections, DH9003 showed no harmful effects on the intestine and liver of rats, but rather resulted in a denser and longer intestinal mucosa, as well as improving the intestinal mucosa of rats. Considering their substantial prospective applications, we concluded that these three isolates are potential probiotic candidates. |
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| language | English |
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| spelling | doaj.art-76c9689bc8b74e44b9295475f561084d2023-11-17T20:31:28ZengMDPI AGMicroorganisms2076-26072023-03-0111484910.3390/microorganisms11040849Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic PotentialsZhenzhu Yang0Ling Ni1Wei Tian2Hai Chi3East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaEast China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, ChinaIn the current study, we screened 46 isolates of lactic acid bacteria (LAB) derived from goat milk for bacteriocin producers that can inhibit common foodborne pathogens (<i>Staphylococcus aureus</i>, <i>Listeria monocytogenes</i>, and <i>Bacillus cereus</i>). The following three strains that showed antimicrobial activity against all indicators were identified: <i>Enterococcus faecalis</i> DH9003 and DH9012, and <i>Lactococcus lactis</i> DH9011. Their antimicrobial products exhibited typical bacteriocin characteristics, such as heat stability and proteinase nature. The bacteriostatic activity of concentrated bacteriocins produced by these LAB was observed at low concentrations (half-minimum inhibitory concentration [MIC50] and 4MIC50), whereas complete inhibition activity against <i>Listeria monocytogenes</i> was detected at high concentrations (16MIC50) of the two <i>Enterococcus faecalis</i> strains (DH9003 and DH9012). Furthermore, the probiotic potentials of the three strains were investigated and described. The results revealed that none of the strains had hemolytic activity, whereas all: were sensitive to ampicillin (50 mg/mL) and streptomycin sulfate (100 mg/mL); were resistant to bile, artificial simulated intestinal tract, and gastric juice at different pH levels (2.5, 3.0, 3.5); and had β-galactosidase activity. Furthermore, all strains exhibited an auto-aggregating phenotype, with self-aggregation ranging from 30% to 55%. DH9003 and DH9012 co-aggregated well with <i>Listeria monocytogenes</i> and <i>Escherichia coli</i> (52.6% and 63.2%, 68.5% and 57.6%, respectively), whereas DH9011 co-aggregated poorly with <i>Listeria monocytogenes</i> (15.6%) and did not co-aggregate with Escherichia coli. Furthermore, our results showed that all three isolates exhibited strong antibacterial activity, tolerance to bile and simulated gastrointestinal environments, adhesion capability, and safety. Finally, DH9003 was selected and used for gavage in rats. By observing the pathological characteristics of rat intestinal and liver tissue sections, DH9003 showed no harmful effects on the intestine and liver of rats, but rather resulted in a denser and longer intestinal mucosa, as well as improving the intestinal mucosa of rats. Considering their substantial prospective applications, we concluded that these three isolates are potential probiotic candidates.https://www.mdpi.com/2076-2607/11/4/849lactic acid bacteriabacteriocin-like substanceprobioticsantimicrobial characteristicsprobiotic potential |
| spellingShingle | Zhenzhu Yang Ling Ni Wei Tian Hai Chi Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials Microorganisms lactic acid bacteria bacteriocin-like substance probiotics antimicrobial characteristics probiotic potential |
| title | Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials |
| title_full | Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials |
| title_fullStr | Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials |
| title_full_unstemmed | Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials |
| title_short | Screening and Identification of Goat-Milk-Derived Lactic Acid Bacteria with Bacteriocin-like Activity and Probiotic Potentials |
| title_sort | screening and identification of goat milk derived lactic acid bacteria with bacteriocin like activity and probiotic potentials |
| topic | lactic acid bacteria bacteriocin-like substance probiotics antimicrobial characteristics probiotic potential |
| url | https://www.mdpi.com/2076-2607/11/4/849 |
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