The Mycorrizal Status in Vineyards Affected by Esca
In this work we analyzed the relationship among native arbuscular mycorrhizal fungi (AMF) and vine roots affected by esca, a serious grapevine trunk disease. The AMF symbiosis was analyzed on the roots of neighboring plants (symptomatic and asymptomatic to esca) in 14 sites of three vineyards in Mar...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/10/869 |
| _version_ | 1797514177747615744 |
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| author | Lucia Landi Renzo Foglia Sergio Murolo Gianfranco Romanazzi |
| author_facet | Lucia Landi Renzo Foglia Sergio Murolo Gianfranco Romanazzi |
| author_sort | Lucia Landi |
| collection | DOAJ |
| description | In this work we analyzed the relationship among native arbuscular mycorrhizal fungi (AMF) and vine roots affected by esca, a serious grapevine trunk disease. The AMF symbiosis was analyzed on the roots of neighboring plants (symptomatic and asymptomatic to esca) in 14 sites of three vineyards in Marche region (central–eastern Italy). The AMF colonization intensity, identified by non-vital staining, showed higher value in all esca symptomatic plants (ranging from 24.6% to 61.3%) than neighboring asymptomatic plants (from 17.4% to 57.6%). The same trend of Glomeromycota phylum abundance was detected by analyzing fungal operational taxonomic units (OTUs) linked to the AMF community, obtained by amplicon high throughput analysis of ITS 1 region. Overall, the highest amount of OTUs was detected on roots from symptomatic plants (0.42%), compared to asymptomatic roots (0.29%). Specific primer pairs for native <i>Rhizophagus irregularis</i> and <i>Funneliformis mosseae</i> AMF species, were designed in 28S rRNA and large subunit (LSU) ribosomal RNA, respectively, and droplet digital PCR protocol for absolute quantification was set up. A higher number of DNA copies of both fungal species were detected more frequently in symptomatic than asymptomatic vines. Our study suggests a relationship between esca and native AMF in grapevine. These results underline the importance of native rhizosphere microbial communities for a better knowledge of grapevine esca disease. |
| first_indexed | 2024-03-10T06:27:56Z |
| format | Article |
| id | doaj.art-76ca760628564ae898371ced90023be9 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T06:27:56Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-76ca760628564ae898371ced90023be92023-11-22T18:47:49ZengMDPI AGJournal of Fungi2309-608X2021-10-0171086910.3390/jof7100869The Mycorrizal Status in Vineyards Affected by EscaLucia Landi0Renzo Foglia1Sergio Murolo2Gianfranco Romanazzi3Department of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, I-60131 Ancona, ItalyDepartment of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, I-60131 Ancona, ItalyDepartment of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, I-60131 Ancona, ItalyDepartment of Agricultural, Food and Environmental Sciences, Marche Polytechnic University, Via Brecce Bianche, I-60131 Ancona, ItalyIn this work we analyzed the relationship among native arbuscular mycorrhizal fungi (AMF) and vine roots affected by esca, a serious grapevine trunk disease. The AMF symbiosis was analyzed on the roots of neighboring plants (symptomatic and asymptomatic to esca) in 14 sites of three vineyards in Marche region (central–eastern Italy). The AMF colonization intensity, identified by non-vital staining, showed higher value in all esca symptomatic plants (ranging from 24.6% to 61.3%) than neighboring asymptomatic plants (from 17.4% to 57.6%). The same trend of Glomeromycota phylum abundance was detected by analyzing fungal operational taxonomic units (OTUs) linked to the AMF community, obtained by amplicon high throughput analysis of ITS 1 region. Overall, the highest amount of OTUs was detected on roots from symptomatic plants (0.42%), compared to asymptomatic roots (0.29%). Specific primer pairs for native <i>Rhizophagus irregularis</i> and <i>Funneliformis mosseae</i> AMF species, were designed in 28S rRNA and large subunit (LSU) ribosomal RNA, respectively, and droplet digital PCR protocol for absolute quantification was set up. A higher number of DNA copies of both fungal species were detected more frequently in symptomatic than asymptomatic vines. Our study suggests a relationship between esca and native AMF in grapevine. These results underline the importance of native rhizosphere microbial communities for a better knowledge of grapevine esca disease.https://www.mdpi.com/2309-608X/7/10/869droplet digital PCRescagrapevine rootsmycorrhizal non-vital stainingnative AMFoperational taxonomic units (OTUs) |
| spellingShingle | Lucia Landi Renzo Foglia Sergio Murolo Gianfranco Romanazzi The Mycorrizal Status in Vineyards Affected by Esca Journal of Fungi droplet digital PCR esca grapevine roots mycorrhizal non-vital staining native AMF operational taxonomic units (OTUs) |
| title | The Mycorrizal Status in Vineyards Affected by Esca |
| title_full | The Mycorrizal Status in Vineyards Affected by Esca |
| title_fullStr | The Mycorrizal Status in Vineyards Affected by Esca |
| title_full_unstemmed | The Mycorrizal Status in Vineyards Affected by Esca |
| title_short | The Mycorrizal Status in Vineyards Affected by Esca |
| title_sort | mycorrizal status in vineyards affected by esca |
| topic | droplet digital PCR esca grapevine roots mycorrhizal non-vital staining native AMF operational taxonomic units (OTUs) |
| url | https://www.mdpi.com/2309-608X/7/10/869 |
| work_keys_str_mv | AT lucialandi themycorrizalstatusinvineyardsaffectedbyesca AT renzofoglia themycorrizalstatusinvineyardsaffectedbyesca AT sergiomurolo themycorrizalstatusinvineyardsaffectedbyesca AT gianfrancoromanazzi themycorrizalstatusinvineyardsaffectedbyesca AT lucialandi mycorrizalstatusinvineyardsaffectedbyesca AT renzofoglia mycorrizalstatusinvineyardsaffectedbyesca AT sergiomurolo mycorrizalstatusinvineyardsaffectedbyesca AT gianfrancoromanazzi mycorrizalstatusinvineyardsaffectedbyesca |