RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts
Abstract Carcinogenesis is a complex molecular process starting with genetic and epigenetic alterations, mutation stimulation, and DNA modification, which leads to proteomic adaptation ending with an uncontrolled proliferation mechanism. The current research focused on the empirical modelling of the...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/srep46603 |
| _version_ | 1827866410143973376 |
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| author | Muhammad Fakhar-e-Alam M. Waseem Akram Seemab Iqbal K. S. Alimgeer M. Atif K. Sultana M. Willander Zhiming M. Wang |
| author_facet | Muhammad Fakhar-e-Alam M. Waseem Akram Seemab Iqbal K. S. Alimgeer M. Atif K. Sultana M. Willander Zhiming M. Wang |
| author_sort | Muhammad Fakhar-e-Alam |
| collection | DOAJ |
| description | Abstract Carcinogenesis is a complex molecular process starting with genetic and epigenetic alterations, mutation stimulation, and DNA modification, which leads to proteomic adaptation ending with an uncontrolled proliferation mechanism. The current research focused on the empirical modelling of the physiological response of human melanoma cells (FM55P) and human foreskin fibroblasts cells (AG01518) to the multilayer zinc oxide (ZnO) nanomaterials under UV-A exposure. To validate this experimental scheme, multilayer ZnO nanomaterials were grown on a femtotip silver capillary and conjugated with protoporphyrin IX (PpIX). Furthermore, PpIX-conjugated ZnO nanomaterials grown on the probe were inserted into human melanoma (FM55P) and foreskin fibroblasts cells (AG01518) under UV-A light exposure. Interestingly, significant cell necrosis was observed because of a loss in mitochondrial membrane potential just after insertion of the femtotip tool. Intense reactive oxygen species (ROS) fluorescence was observed after exposure to the ZnO NWs conjugated with PpIX femtotip model under UV exposure. Results were verified by applying several experimental techniques, e.g., ROS detection, MTT assay, and fluorescence spectroscopy. The present work reports experimental modelling of cell necrosis in normal human skin as well as a cancerous tissue. These obtained results pave the way for a more rational strategy for biomedical and clinical applications. |
| first_indexed | 2024-03-12T15:03:28Z |
| format | Article |
| id | doaj.art-76cdca02176842a687af215c54d8dc79 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T15:03:28Z |
| publishDate | 2017-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-76cdca02176842a687af215c54d8dc792023-08-13T11:13:53ZengNature PortfolioScientific Reports2045-23222017-04-017111310.1038/srep46603RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin FibroblastsMuhammad Fakhar-e-Alam0M. Waseem Akram1Seemab Iqbal2K. S. Alimgeer3M. Atif4K. Sultana5M. Willander6Zhiming M. Wang7Institute of Fundamental and Frontier Science, University of Electronic Science and Technology of ChinaInstitute of Fundamental and Frontier Science, University of Electronic Science and Technology of ChinaDepartment of Physics, GC UniversityCOMSATS Institute of Information TechnologyDepartment of Physics and Astronomy, College of Science, King Saud UniversityDepartment of Science and Technology, Campus Norrköping, Linköping UniversityDepartment of Science and Technology, Campus Norrköping, Linköping UniversityInstitute of Fundamental and Frontier Science, University of Electronic Science and Technology of ChinaAbstract Carcinogenesis is a complex molecular process starting with genetic and epigenetic alterations, mutation stimulation, and DNA modification, which leads to proteomic adaptation ending with an uncontrolled proliferation mechanism. The current research focused on the empirical modelling of the physiological response of human melanoma cells (FM55P) and human foreskin fibroblasts cells (AG01518) to the multilayer zinc oxide (ZnO) nanomaterials under UV-A exposure. To validate this experimental scheme, multilayer ZnO nanomaterials were grown on a femtotip silver capillary and conjugated with protoporphyrin IX (PpIX). Furthermore, PpIX-conjugated ZnO nanomaterials grown on the probe were inserted into human melanoma (FM55P) and foreskin fibroblasts cells (AG01518) under UV-A light exposure. Interestingly, significant cell necrosis was observed because of a loss in mitochondrial membrane potential just after insertion of the femtotip tool. Intense reactive oxygen species (ROS) fluorescence was observed after exposure to the ZnO NWs conjugated with PpIX femtotip model under UV exposure. Results were verified by applying several experimental techniques, e.g., ROS detection, MTT assay, and fluorescence spectroscopy. The present work reports experimental modelling of cell necrosis in normal human skin as well as a cancerous tissue. These obtained results pave the way for a more rational strategy for biomedical and clinical applications.https://doi.org/10.1038/srep46603 |
| spellingShingle | Muhammad Fakhar-e-Alam M. Waseem Akram Seemab Iqbal K. S. Alimgeer M. Atif K. Sultana M. Willander Zhiming M. Wang RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts Scientific Reports |
| title | RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts |
| title_full | RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts |
| title_fullStr | RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts |
| title_full_unstemmed | RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts |
| title_short | RETRACTED ARTICLE: Empirical Modeling of Physiochemical Immune Response of Multilayer Zinc Oxide Nanomaterials under UV Exposure to Melanoma and Foreskin Fibroblasts |
| title_sort | retracted article empirical modeling of physiochemical immune response of multilayer zinc oxide nanomaterials under uv exposure to melanoma and foreskin fibroblasts |
| url | https://doi.org/10.1038/srep46603 |
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