Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos

Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos

Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled the induction of site-specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious problem for genetically modified pigs. In the present study, we investigated suitable timing and concentration of CRISPR/C...

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Bibliographic Details
Main Authors: Fuminori TANIHARA, Maki HIRATA, Nhien Thi NGUYEN, Quynh Anh LE, Takayuki HIRANO, Takeshige OTOI
Format: Article
Language:English
Published: The Society for Reproduction and Development 2019-02-01
Series:The Journal of Reproduction and Development
Subjects:
crispr/cas9
cytoplasmic microinjection
gene editing
mosaicism
porcine zygote
Online Access:https://www.jstage.jst.go.jp/article/jrd/65/3/65_2018-116/_pdf/-char/en

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