Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay

Lupine is a legume and important hidden food allergen. In the present study, our aim was to investigate the presence of lupine in some foodstuffs including 90 packaged food samples (breakfast cereals, oatmeals, biscuits, and chocolate) belonging to different firms in Turkey. Extraction and determina...

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Main Authors: Nursel GÜNAY, Buket ER DEMİRHAN, Burak DEMİRHAN
Format: Article
Language:English
Published: AcademicPres 2022-05-01
Series:Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
Subjects:
Online Access:https://journals.usamvcluj.ro/index.php/fst/article/view/14409
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author Nursel GÜNAY
Buket ER DEMİRHAN
Burak DEMİRHAN
author_facet Nursel GÜNAY
Buket ER DEMİRHAN
Burak DEMİRHAN
author_sort Nursel GÜNAY
collection DOAJ
description Lupine is a legume and important hidden food allergen. In the present study, our aim was to investigate the presence of lupine in some foodstuffs including 90 packaged food samples (breakfast cereals, oatmeals, biscuits, and chocolate) belonging to different firms in Turkey. Extraction and determination of lupine in all samples were made by the enzyme-linked immunosorbent assay (ELISA) technique. The presence of lupine was detected in 11 (12.22%) of 90 food samples. Lupine was not detected in the samples of breakfast cereal and oatmeal. The mean levels (±S.E) of positive lupine of biscuits and chocolate samples were found to be 1.53±0.18 mg kg-1 and 2.04±0.16 mg kg-1, respectively. It was declared on the labels of some of the analyzed products that they may contain traces of lupine. Lupine was detected in only one of these samples. There was no information about the presence of lupine on the labels of other lupine detected samples. As a result, the importance of the detection of allergen substances in terms of food safety, food quality, and public health and the importance of food labeling are emphasized in this study. Analytical methods for monitoring allergens in risky foods are important.
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spelling doaj.art-76d2c7886d5a4bc38787a040adb3e5b92022-12-22T03:26:08ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology2344-23442344-53002022-05-01179364010.15835/buasvmcn-fst:2022.000611731Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent AssayNursel GÜNAY0Buket ER DEMİRHAN1Burak DEMİRHAN2Rebuplic of Turkey Ministry of Health, Health Services General DirectorateGazi University Faculty of Pharmacy, Department of Pharmaceutical Basic SciencesGazi University Faculty of Pharmacy, Department of Pharmaceutical Basic SciencesLupine is a legume and important hidden food allergen. In the present study, our aim was to investigate the presence of lupine in some foodstuffs including 90 packaged food samples (breakfast cereals, oatmeals, biscuits, and chocolate) belonging to different firms in Turkey. Extraction and determination of lupine in all samples were made by the enzyme-linked immunosorbent assay (ELISA) technique. The presence of lupine was detected in 11 (12.22%) of 90 food samples. Lupine was not detected in the samples of breakfast cereal and oatmeal. The mean levels (±S.E) of positive lupine of biscuits and chocolate samples were found to be 1.53±0.18 mg kg-1 and 2.04±0.16 mg kg-1, respectively. It was declared on the labels of some of the analyzed products that they may contain traces of lupine. Lupine was detected in only one of these samples. There was no information about the presence of lupine on the labels of other lupine detected samples. As a result, the importance of the detection of allergen substances in terms of food safety, food quality, and public health and the importance of food labeling are emphasized in this study. Analytical methods for monitoring allergens in risky foods are important.https://journals.usamvcluj.ro/index.php/fst/article/view/14409food allergenlupineelisa
spellingShingle Nursel GÜNAY
Buket ER DEMİRHAN
Burak DEMİRHAN
Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Food Science and Technology
food allergen
lupine
elisa
title Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
title_full Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
title_fullStr Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
title_full_unstemmed Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
title_short Investigation of Lupine Allergen Presence in Some Food Products by Enzyme-Linked Immunosorbent Assay
title_sort investigation of lupine allergen presence in some food products by enzyme linked immunosorbent assay
topic food allergen
lupine
elisa
url https://journals.usamvcluj.ro/index.php/fst/article/view/14409
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AT buketerdemirhan investigationoflupineallergenpresenceinsomefoodproductsbyenzymelinkedimmunosorbentassay
AT burakdemirhan investigationoflupineallergenpresenceinsomefoodproductsbyenzymelinkedimmunosorbentassay