Role of the pre-initiation complex in Mediator recruitment and dynamics
The Mediator complex stimulates the cooperative assembly of a pre-initiation complex (PIC) and recruitment of RNA Polymerase II (Pol II) for gene activation. The core Mediator complex is organized into head, middle, and tail modules, and in budding yeast (Saccharomyces cerevisiae), Mediator recruitm...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/39633 |
| _version_ | 1818028597216018432 |
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| author | Elisabeth R Knoll Z Iris Zhu Debasish Sarkar David Landsman Randall H Morse |
| author_facet | Elisabeth R Knoll Z Iris Zhu Debasish Sarkar David Landsman Randall H Morse |
| author_sort | Elisabeth R Knoll |
| collection | DOAJ |
| description | The Mediator complex stimulates the cooperative assembly of a pre-initiation complex (PIC) and recruitment of RNA Polymerase II (Pol II) for gene activation. The core Mediator complex is organized into head, middle, and tail modules, and in budding yeast (Saccharomyces cerevisiae), Mediator recruitment has generally been ascribed to sequence-specific activators engaging the tail module triad of Med2-Med3-Med15 at upstream activating sequences (UASs). We show that yeast lacking Med2-Med3-Med15 are viable and that Mediator and PolII are recruited to promoters genome-wide in these cells, albeit at reduced levels. To test whether Mediator might alternatively be recruited via interactions with the PIC, we examined Mediator association genome-wide after depleting PIC components. We found that depletion of Taf1, Rpb3, and TBP profoundly affected Mediator association at active gene promoters, with TBP being critical for transit of Mediator from UAS to promoter, while Pol II and Taf1 stabilize Mediator association at proximal promoters. |
| first_indexed | 2024-12-10T05:06:19Z |
| format | Article |
| id | doaj.art-76d42f78416a4d73a3d85cbf80c424bc |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T05:06:19Z |
| publishDate | 2018-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-76d42f78416a4d73a3d85cbf80c424bc2022-12-22T02:01:15ZengeLife Sciences Publications LtdeLife2050-084X2018-12-01710.7554/eLife.39633Role of the pre-initiation complex in Mediator recruitment and dynamicsElisabeth R Knoll0https://orcid.org/0000-0002-1083-6472Z Iris Zhu1Debasish Sarkar2David Landsman3https://orcid.org/0000-0002-9819-6675Randall H Morse4https://orcid.org/0000-0003-0000-8718Department of Biomedical Sciences, School of Public Health, University at Albany, Albany, United StatesComputational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, Bethesda, United StatesWadsworth Center, New York State Department of Health, Albany, United StatesComputational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, Bethesda, United StatesDepartment of Biomedical Sciences, School of Public Health, University at Albany, Albany, United States; Wadsworth Center, New York State Department of Health, Albany, United StatesThe Mediator complex stimulates the cooperative assembly of a pre-initiation complex (PIC) and recruitment of RNA Polymerase II (Pol II) for gene activation. The core Mediator complex is organized into head, middle, and tail modules, and in budding yeast (Saccharomyces cerevisiae), Mediator recruitment has generally been ascribed to sequence-specific activators engaging the tail module triad of Med2-Med3-Med15 at upstream activating sequences (UASs). We show that yeast lacking Med2-Med3-Med15 are viable and that Mediator and PolII are recruited to promoters genome-wide in these cells, albeit at reduced levels. To test whether Mediator might alternatively be recruited via interactions with the PIC, we examined Mediator association genome-wide after depleting PIC components. We found that depletion of Taf1, Rpb3, and TBP profoundly affected Mediator association at active gene promoters, with TBP being critical for transit of Mediator from UAS to promoter, while Pol II and Taf1 stabilize Mediator association at proximal promoters.https://elifesciences.org/articles/39633mediatortranscription factorsTATA-binding proteinpre-initiation complexyeastChIP-seq |
| spellingShingle | Elisabeth R Knoll Z Iris Zhu Debasish Sarkar David Landsman Randall H Morse Role of the pre-initiation complex in Mediator recruitment and dynamics eLife mediator transcription factors TATA-binding protein pre-initiation complex yeast ChIP-seq |
| title | Role of the pre-initiation complex in Mediator recruitment and dynamics |
| title_full | Role of the pre-initiation complex in Mediator recruitment and dynamics |
| title_fullStr | Role of the pre-initiation complex in Mediator recruitment and dynamics |
| title_full_unstemmed | Role of the pre-initiation complex in Mediator recruitment and dynamics |
| title_short | Role of the pre-initiation complex in Mediator recruitment and dynamics |
| title_sort | role of the pre initiation complex in mediator recruitment and dynamics |
| topic | mediator transcription factors TATA-binding protein pre-initiation complex yeast ChIP-seq |
| url | https://elifesciences.org/articles/39633 |
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