Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation
Introduction: Medical devices are made from a variety of materials such as polypropylene, polycarbonate, poly styrene, glass and etc. by attaching to this surfaces, Acinetobacter baumannii can form biofilms and then cause several device associated infections. Biofilms are communities of bacteria a...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2014-01-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_id=194&slc_lang=en&sid=1&ftxt=1 |
| _version_ | 1818236834239479808 |
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| author | Saghar Hendiani Ahya Abdi-Ali Parisa Mohammadi |
| author_facet | Saghar Hendiani Ahya Abdi-Ali Parisa Mohammadi |
| author_sort | Saghar Hendiani |
| collection | DOAJ |
| description | Introduction: Medical devices are made from a variety of materials such as polypropylene, polycarbonate, poly styrene, glass and etc. by attaching to this surfaces, Acinetobacter baumannii can form biofilms and then cause several device associated infections. Biofilms are communities of bacteria attached to the surfaces. In this study, biofilm formation ability in clinical isolates of Acinetobacter baumannii was assessed by two methods on different surfaces.
Materials and methods: Biofilm formation by 75 clinical isolates of A. baumannii was evaluated on polycarbonate surface (microtiter plate) and polypropylene surface (falcon) by crystal violet and 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT tetrazolium sodium salt) assay methods. Falcon or tube method was carried out under static and agitation conditions.
Results: Results showed the most isolates can form biofilm but higher numbers of isolates form biofilm on polypropylene surface under agitation. XTT method confirmed strong biofilm formation ability of 10 isolates.
Discussion and conclusion: Each of the two assays showed an excellent applicability for the quantification of biofilms. The Crystal violet assay is cheap, easy and is usually used for the quantification of biofilms formed by microorganisms but XTT is more reliable and repeatable. Most of A. baumannii isolates have potential to form biofilm on the medical devices which may result in device-associated infections. |
| first_indexed | 2024-12-12T12:16:09Z |
| format | Article |
| id | doaj.art-76d81a87da4546a5a1d209da1f231a7f |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-12T12:16:09Z |
| publishDate | 2014-01-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-76d81a87da4546a5a1d209da1f231a7f2022-12-22T00:24:45ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812014-01-01285156Comparison of two methods for quantification of Acinetobacter baumannii biofilm formationSaghar Hendiani0Ahya Abdi-Ali1Parisa Mohammadi2MSc of Microbiology, Alzahra University, Tehran, Iran, saghar.hendiani@yahoo.comAssociate professor of Microbiology, Alzahra University, Tehran, Iran, abdialya@alzahra.ac.irAssistant professor of Microbiology, Alzahra University, Tehran, Iran, mohamadi_p@yahoo.comIntroduction: Medical devices are made from a variety of materials such as polypropylene, polycarbonate, poly styrene, glass and etc. by attaching to this surfaces, Acinetobacter baumannii can form biofilms and then cause several device associated infections. Biofilms are communities of bacteria attached to the surfaces. In this study, biofilm formation ability in clinical isolates of Acinetobacter baumannii was assessed by two methods on different surfaces. Materials and methods: Biofilm formation by 75 clinical isolates of A. baumannii was evaluated on polycarbonate surface (microtiter plate) and polypropylene surface (falcon) by crystal violet and 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide salt (XTT tetrazolium sodium salt) assay methods. Falcon or tube method was carried out under static and agitation conditions. Results: Results showed the most isolates can form biofilm but higher numbers of isolates form biofilm on polypropylene surface under agitation. XTT method confirmed strong biofilm formation ability of 10 isolates. Discussion and conclusion: Each of the two assays showed an excellent applicability for the quantification of biofilms. The Crystal violet assay is cheap, easy and is usually used for the quantification of biofilms formed by microorganisms but XTT is more reliable and repeatable. Most of A. baumannii isolates have potential to form biofilm on the medical devices which may result in device-associated infections.http://uijs.ui.ac.ir/bjm/browse.php?a_id=194&slc_lang=en&sid=1&ftxt=1BiofilmCrystal violetAcinetobacter baumanniiXTT |
| spellingShingle | Saghar Hendiani Ahya Abdi-Ali Parisa Mohammadi Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation Biological Journal of Microorganism Biofilm Crystal violet Acinetobacter baumannii XTT |
| title | Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation |
| title_full | Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation |
| title_fullStr | Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation |
| title_full_unstemmed | Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation |
| title_short | Comparison of two methods for quantification of Acinetobacter baumannii biofilm formation |
| title_sort | comparison of two methods for quantification of acinetobacter baumannii biofilm formation |
| topic | Biofilm Crystal violet Acinetobacter baumannii XTT |
| url | http://uijs.ui.ac.ir/bjm/browse.php?a_id=194&slc_lang=en&sid=1&ftxt=1 |
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