Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence
Abstract Background Pancreatic cancer is the foremost contributor to cancer-related deaths globally, and its prevalence continues to rise annually. Nevertheless, the underlying mechanisms behind its development remain unclear and necessitate comprehensive investigation. Methods In this study, a tota...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-04-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-024-12202-z |
| _version_ | 1797199338244407296 |
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| author | Feng Zhao Anli Chen Xiaotian Wu Xiangyu Deng Jiali Yang Jianjiang Xue |
| author_facet | Feng Zhao Anli Chen Xiaotian Wu Xiangyu Deng Jiali Yang Jianjiang Xue |
| author_sort | Feng Zhao |
| collection | DOAJ |
| description | Abstract Background Pancreatic cancer is the foremost contributor to cancer-related deaths globally, and its prevalence continues to rise annually. Nevertheless, the underlying mechanisms behind its development remain unclear and necessitate comprehensive investigation. Methods In this study, a total of 29 fresh stool samples were collected from patients diagnosed with pancreatic cancer. The gut microbial data of healthy controls were obtained from the SRA database (SRA data number: SRP150089). Additionally, 28 serum samples and diseased tissues were collected from 14 patients with confirmed pancreatic cancer and 14 patients with chronic pancreatitis. Informed consent was obtained from both groups of patients. Microbial sequencing was performed using 16s rRNA. Results The results showed that compared with healthy controls, the species abundance index of intestinal flora in patients with pancreatic cancer was increased (P < 0.05), and the number of beneficial bacteria at the genus level was reduced (P < 0.05). Compared with patients with chronic pancreatitis, the expression levels of CA242 and CA199 in the serum of patients with pancreatic cancer were increased (P < 0.05). The bacterial richness index of tumor microorganisms in patients with pancreatic cancer increased, while the diversity index decreased(P < 0.05). Furthermore, there was a change in the species composition at the genus level. Additionally, the expression level of CA242 was found to be significantly positively correlated with the relative abundance of Acinetobacter(P < 0.05). Conclusion Over all, the expression levels of serum tumor markers CA242 and CA19-9 in patients with pancreatic cancer are increased, while the beneficial bacteria in the intestine and tumor microenvironment are reduced and pathogenic bacteria are increased. Acinetobacter is a specific bacterial genus highly expressed in pancreatic cancer tissue. |
| first_indexed | 2024-04-24T07:14:10Z |
| format | Article |
| id | doaj.art-76d8d650d0f9466f902e0e87fee8fb77 |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-24T07:14:10Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-76d8d650d0f9466f902e0e87fee8fb772024-04-21T11:21:53ZengBMCBMC Cancer1471-24072024-04-0124111010.1186/s12885-024-12202-zHeterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidenceFeng Zhao0Anli Chen1Xiaotian Wu2Xiangyu Deng3Jiali Yang4Jianjiang Xue5Department of Health Laboratory Technology, School of Public Health, Chongqing Medical UniversityDepartment of Clinical Laboratory, University-Town Hospital of Chongqing Medical UniversityDepartment of Clinical Laboratory, University-Town Hospital of Chongqing Medical UniversityDepartment of Clinical Laboratory, University-Town Hospital of Chongqing Medical UniversityInstitute of Hepatopancreatobiliary Surgery, Chongqing General HospitalDepartment of Health Laboratory Technology, School of Public Health, Chongqing Medical UniversityAbstract Background Pancreatic cancer is the foremost contributor to cancer-related deaths globally, and its prevalence continues to rise annually. Nevertheless, the underlying mechanisms behind its development remain unclear and necessitate comprehensive investigation. Methods In this study, a total of 29 fresh stool samples were collected from patients diagnosed with pancreatic cancer. The gut microbial data of healthy controls were obtained from the SRA database (SRA data number: SRP150089). Additionally, 28 serum samples and diseased tissues were collected from 14 patients with confirmed pancreatic cancer and 14 patients with chronic pancreatitis. Informed consent was obtained from both groups of patients. Microbial sequencing was performed using 16s rRNA. Results The results showed that compared with healthy controls, the species abundance index of intestinal flora in patients with pancreatic cancer was increased (P < 0.05), and the number of beneficial bacteria at the genus level was reduced (P < 0.05). Compared with patients with chronic pancreatitis, the expression levels of CA242 and CA199 in the serum of patients with pancreatic cancer were increased (P < 0.05). The bacterial richness index of tumor microorganisms in patients with pancreatic cancer increased, while the diversity index decreased(P < 0.05). Furthermore, there was a change in the species composition at the genus level. Additionally, the expression level of CA242 was found to be significantly positively correlated with the relative abundance of Acinetobacter(P < 0.05). Conclusion Over all, the expression levels of serum tumor markers CA242 and CA19-9 in patients with pancreatic cancer are increased, while the beneficial bacteria in the intestine and tumor microenvironment are reduced and pathogenic bacteria are increased. Acinetobacter is a specific bacterial genus highly expressed in pancreatic cancer tissue.https://doi.org/10.1186/s12885-024-12202-zPancreatic cancerTumor microorganismsGut microbesTumor markers |
| spellingShingle | Feng Zhao Anli Chen Xiaotian Wu Xiangyu Deng Jiali Yang Jianjiang Xue Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence BMC Cancer Pancreatic cancer Tumor microorganisms Gut microbes Tumor markers |
| title | Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence |
| title_full | Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence |
| title_fullStr | Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence |
| title_full_unstemmed | Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence |
| title_short | Heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer: insights from clinical evidence |
| title_sort | heterogeneous changes in gut and tumor microbiota in patients with pancreatic cancer insights from clinical evidence |
| topic | Pancreatic cancer Tumor microorganisms Gut microbes Tumor markers |
| url | https://doi.org/10.1186/s12885-024-12202-z |
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