Protocol for morphometric analysis of neurons derived from human pluripotent stem cells

Protocol for morphometric analysis of neurons derived from human pluripotent stem cells

Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of...

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Bibliographic Details
Main Authors: Sofía Mucci, María Soledad Rodriguez-Varela, Luciana Isaja, Sofía Luján Ferriol-Laffouillere, Gustavo Emilio Sevlever, María Elida Scassa, Leonardo Romorini.
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Microscopy
Molecular Biology
Neuroscience
Stem Cells
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003677
Description
Summary:Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
ISSN:2666-1667

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