Protocol for morphometric analysis of neurons derived from human pluripotent stem cells

Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of...

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Main Authors: Sofía Mucci, María Soledad Rodriguez-Varela, Luciana Isaja, Sofía Luján Ferriol-Laffouillere, Gustavo Emilio Sevlever, María Elida Scassa, Leonardo Romorini.
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003677
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author Sofía Mucci
María Soledad Rodriguez-Varela
Luciana Isaja
Sofía Luján Ferriol-Laffouillere
Gustavo Emilio Sevlever
María Elida Scassa
Leonardo Romorini.
author_facet Sofía Mucci
María Soledad Rodriguez-Varela
Luciana Isaja
Sofía Luján Ferriol-Laffouillere
Gustavo Emilio Sevlever
María Elida Scassa
Leonardo Romorini.
author_sort Sofía Mucci
collection DOAJ
description Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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spelling doaj.art-76dc47c5474942cc8684d07ed8f3e3b02022-12-22T03:30:24ZengElsevierSTAR Protocols2666-16672022-09-0133101487Protocol for morphometric analysis of neurons derived from human pluripotent stem cellsSofía Mucci0María Soledad Rodriguez-Varela1Luciana Isaja2Sofía Luján Ferriol-Laffouillere3Gustavo Emilio Sevlever4María Elida Scassa5Leonardo Romorini.6Laboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, Argentina; Corresponding authorLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, Argentina; Corresponding authorSummary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003677Cell BiologyMicroscopyMolecular BiologyNeuroscienceStem CellsCell Differentiation
spellingShingle Sofía Mucci
María Soledad Rodriguez-Varela
Luciana Isaja
Sofía Luján Ferriol-Laffouillere
Gustavo Emilio Sevlever
María Elida Scassa
Leonardo Romorini.
Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
STAR Protocols
Cell Biology
Microscopy
Molecular Biology
Neuroscience
Stem Cells
Cell Differentiation
title Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_full Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_fullStr Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_full_unstemmed Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_short Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
title_sort protocol for morphometric analysis of neurons derived from human pluripotent stem cells
topic Cell Biology
Microscopy
Molecular Biology
Neuroscience
Stem Cells
Cell Differentiation
url http://www.sciencedirect.com/science/article/pii/S2666166722003677
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