Protocol for morphometric analysis of neurons derived from human pluripotent stem cells
Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of...
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Format: | Article |
Language: | English |
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Elsevier
2022-09-01
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Series: | STAR Protocols |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722003677 |
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author | Sofía Mucci María Soledad Rodriguez-Varela Luciana Isaja Sofía Luján Ferriol-Laffouillere Gustavo Emilio Sevlever María Elida Scassa Leonardo Romorini. |
author_facet | Sofía Mucci María Soledad Rodriguez-Varela Luciana Isaja Sofía Luján Ferriol-Laffouillere Gustavo Emilio Sevlever María Elida Scassa Leonardo Romorini. |
author_sort | Sofía Mucci |
collection | DOAJ |
description | Summary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
first_indexed | 2024-04-12T13:54:55Z |
format | Article |
id | doaj.art-76dc47c5474942cc8684d07ed8f3e3b0 |
institution | Directory Open Access Journal |
issn | 2666-1667 |
language | English |
last_indexed | 2024-04-12T13:54:55Z |
publishDate | 2022-09-01 |
publisher | Elsevier |
record_format | Article |
series | STAR Protocols |
spelling | doaj.art-76dc47c5474942cc8684d07ed8f3e3b02022-12-22T03:30:24ZengElsevierSTAR Protocols2666-16672022-09-0133101487Protocol for morphometric analysis of neurons derived from human pluripotent stem cellsSofía Mucci0María Soledad Rodriguez-Varela1Luciana Isaja2Sofía Luján Ferriol-Laffouillere3Gustavo Emilio Sevlever4María Elida Scassa5Leonardo Romorini.6Laboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, Argentina; Corresponding authorLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, ArgentinaLaboratorio de Investigaciones Aplicadas en Neurociencias (LIAN), Instituto de Neurociencias (INEU), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI), Escobar, Buenos Aires B1625XAF, Argentina; Corresponding authorSummary: The analysis of morphological features of neurons derived from human pluripotent stem cells (hPSCs) is important to describe neuronal phenotypes and changes observed throughout development. Using free and easily accessible tools, we describe a protocol for the morphometric quantification of hPSCs-derived neurons in two- and three-dimensions in vitro cultures. We detail the analysis of soma area and main and secondary dendrites lengths of GFP-transfected neurons and the measurement of area and perimeter of immunostained neurospheres. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722003677Cell BiologyMicroscopyMolecular BiologyNeuroscienceStem CellsCell Differentiation |
spellingShingle | Sofía Mucci María Soledad Rodriguez-Varela Luciana Isaja Sofía Luján Ferriol-Laffouillere Gustavo Emilio Sevlever María Elida Scassa Leonardo Romorini. Protocol for morphometric analysis of neurons derived from human pluripotent stem cells STAR Protocols Cell Biology Microscopy Molecular Biology Neuroscience Stem Cells Cell Differentiation |
title | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
title_full | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
title_fullStr | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
title_full_unstemmed | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
title_short | Protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
title_sort | protocol for morphometric analysis of neurons derived from human pluripotent stem cells |
topic | Cell Biology Microscopy Molecular Biology Neuroscience Stem Cells Cell Differentiation |
url | http://www.sciencedirect.com/science/article/pii/S2666166722003677 |
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